PMID- 30366204
OWN - NLM
STAT- MEDLINE
DCOM- 20190530
LR  - 20190530
IS  - 1872-6844 (Electronic)
IS  - 0920-1211 (Linking)
VI  - 148
DP  - 2018 Dec
TI  - Overexpression of inflammatory-related and nitric oxide synthase genes in 
      olfactory bulbs from frontal lobe epilepsy patients.
PG  - 37-43
LID - S0920-1211(18)30024-X [pii]
LID - 10.1016/j.eplepsyres.2018.09.012 [doi]
AB  - Neuroinflammation has been shown to constitute a crucial mechanism in the 
      pathophysiology of epileptic brain and several genes of inflammatory mediators 
      have been detected in surgically resected hippocampus tissue but not in 
      non-related seizure brain regions. Interestingly, it has been reported an 
      olfactory dysfunction in frontal lobe epilepsy (FLE). Our aim was to quantify the 
      gene expression of inflammatory-related and nitric oxide synthase genes in 
      olfactory bulbs (OB) tissue from FLE patients. RNA was isolated from OB resection 
      of FLE patients and autopsy subjects without any neurological disease (n = 7, 
      each). After cDNA synthesis, we performed qPCR for interleukin-1beta (IL-1beta), 
      interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), nuclear factor kappaB p65 
      (RELA), Toll-like receptor 4 (TLR 4), its agonist high mobility group box 1 (HMGB 
      1) as well nitric oxide synthase isozymes (NOS 1, 2 and 3). We found a 
      significant increase in gene expression of pro-inflammatory cytokines (IL-1beta, 
      IL-6 and TNFalpha), TLR4 receptor and in its agonist HMGB1 and the downstream 
      transcription factor NFkappaB p65. Moreover, we observed an increase of both NOS1 and 
      NOS3 and a slightly increase of NOS2; however, it was not significant. Our study 
      describes the overexpression of inflammatory-related genes and NOS isozymes in OB 
      from FLE patients. Even though, the number of patients was limited, our findings 
      could point out that neuroinflammation and nitrosative stress-related genes in 
      the OB could be produced in general manner in all brain regions and thus 
      contribute in part, to the olfactory dysfunction observed in FLE patients.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Mercado-Gomez, Octavio Fabian
AU  - Mercado-Gomez OF
AD  - Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma 
      de Mexico, 04510, Ciudad deMexico, Mexico.
FAU - Cordova-Davalos, Laura
AU  - Cordova-Davalos L
AD  - Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma 
      de Mexico, 04510, Ciudad deMexico, Mexico; Departmento de Farmacobiologia, Centro 
      de Investigacion y de Estudios Avanzados, 14330, Ciudad deMexico, Mexico; Seccion 
      de Neurocirugia, Instituto Nacional de Neurologia y Neurocirugia "Manuel Velasco 
      Suarez", 14269, Ciudad deMexico, Mexico.
FAU - Garcia-Betanzo, Delfina
AU  - Garcia-Betanzo D
AD  - Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma 
      de Mexico, 04510, Ciudad deMexico, Mexico.
FAU - Rocha, Luisa
AU  - Rocha L
AD  - Departmento de Farmacobiologia, Centro de Investigacion y de Estudios Avanzados, 
      14330, Ciudad deMexico, Mexico.
FAU - Alonso-Vanegas, Mario Arturo
AU  - Alonso-Vanegas MA
AD  - Seccion de Neurocirugia, Instituto Nacional de Neurologia y Neurocirugia "Manuel 
      Velasco Suarez", 14269, Ciudad deMexico, Mexico.
FAU - Cienfuegos, Jesus
AU  - Cienfuegos J
AD  - Seccion de Neurocirugia, Instituto Nacional de Neurologia y Neurocirugia "Manuel 
      Velasco Suarez", 14269, Ciudad deMexico, Mexico.
FAU - Guevara-Guzman, Rosalinda
AU  - Guevara-Guzman R
AD  - Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma 
      de Mexico, 04510, Ciudad deMexico, Mexico. Electronic address: rguevara@unam.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181009
PL  - Netherlands
TA  - Epilepsy Res
JT  - Epilepsy research
JID - 8703089
RN  - 0 (Cytokines)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Child
MH  - Cytokines/*metabolism
MH  - Drug Resistant Epilepsy/diagnostic imaging/enzymology/immunology/surgery
MH  - Epilepsy, Frontal Lobe/diagnostic imaging/*enzymology/*immunology/surgery
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nitric Oxide Synthase/*metabolism
MH  - Olfactory Bulb/*enzymology/*immunology
MH  - Young Adult
OTO - NOTNLM
OT  - Frontal epilepsy
OT  - Hipoocampus
OT  - NOS
OT  - Olfactory bulb
OT  - Olfactoy disfunction
EDAT- 2018/10/27 06:00
MHDA- 2019/05/31 06:00
CRDT- 2018/10/27 06:00
PHST- 2018/01/11 00:00 [received]
PHST- 2018/09/12 00:00 [revised]
PHST- 2018/09/22 00:00 [accepted]
PHST- 2018/10/27 06:00 [pubmed]
PHST- 2019/05/31 06:00 [medline]
PHST- 2018/10/27 06:00 [entrez]
AID - S0920-1211(18)30024-X [pii]
AID - 10.1016/j.eplepsyres.2018.09.012 [doi]
PST - ppublish
SO  - Epilepsy Res. 2018 Dec;148:37-43. doi: 10.1016/j.eplepsyres.2018.09.012. Epub 
      2018 Oct 9.