PMID- 30367153
OWN - NLM
STAT- MEDLINE
DCOM- 20191015
LR  - 20200601
IS  - 1745-7254 (Electronic)
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 40
IP  - 6
DP  - 2019 Jun
TI  - Propofol decreases the excitability of cholinergic neurons in mouse basal 
      forebrain via GABA(A) receptors.
PG  - 755-761
LID - 10.1038/s41401-018-0168-6 [doi]
AB  - Propofol is an intravenous anesthetic that can active gamma-aminobutyric acid A 
      (GABA(A)) receptors and generate sedative-hypnotic effects. Propofol has been 
      widely applied clinically to achieve sedation comparable to sleep in humans. The 
      basal forebrain (BF) is a brain region that plays an important role in sleep-wake 
      regulation. Previous studies suggest that propofol affects the sleep-wake circuit 
      via the BF; however, the mechanism remains elusive. In the current study we 
      investigated the effects of propofol on the inherent properties of cholinergic 
      neurons and their ability to convert excitatory inputs into spikes in mouse BF 
      slices using whole-cell patch clamp recordings. Bath application of propofol 
      (10 muM) significantly elevated the threshold potentials (Vts), decreased the 
      number of spikes in response to a depolarizing current injection, and augmented 
      the inter-spike intervals (ISIs), energy barrier (Vts-Vrs), and absolute 
      refractory periods (ARPs). These effects were eliminated by co-application of a 
      GABA(A) receptor antagonist picrotoxin (50 muM). Altogether, our results reveal 
      that propofol decreases the excitability of cholinergic neurons in mouse BF via 
      GABA(A) receptors.
FAU - Chen, Lei
AU  - Chen L
AD  - Department of Pharmacology and Physiology, School of Basic Medical Sciences, 
      Anhui Medical University, Hefei, 230032, China.
FAU - Yang, Zhi-Lai
AU  - Yang ZL
AD  - Department of Anesthesiology, First Affiliated Hospital of Anhui Medical 
      University, Hefei, 230022, China.
FAU - Cheng, Juan
AU  - Cheng J
AD  - Department of Pharmacology and Physiology, School of Basic Medical Sciences, 
      Anhui Medical University, Hefei, 230032, China.
FAU - Zhang, Ping-Ping
AU  - Zhang PP
AD  - Department of Pharmacology and Physiology, School of Basic Medical Sciences, 
      Anhui Medical University, Hefei, 230032, China.
FAU - Zhang, Le-Sha
AU  - Zhang LS
AD  - Department of Pharmacology and Physiology, School of Basic Medical Sciences, 
      Anhui Medical University, Hefei, 230032, China.
FAU - Liu, Xue-Sheng
AU  - Liu XS
AD  - Department of Anesthesiology, First Affiliated Hospital of Anhui Medical 
      University, Hefei, 230022, China. liu711029@hotmail.com.
FAU - Wang, Lie-Cheng
AU  - Wang LC
AD  - Department of Pharmacology and Physiology, School of Basic Medical Sciences, 
      Anhui Medical University, Hefei, 230032, China. wangliecheng@ahmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20181026
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Anesthetics, Intravenous)
RN  - 0 (Hypnotics and Sedatives)
RN  - 0 (Receptors, GABA-A)
RN  - YI7VU623SF (Propofol)
SB  - IM
MH  - Anesthetics, Intravenous/*pharmacology
MH  - Animals
MH  - Basal Forebrain/*drug effects
MH  - Cholinergic Neurons/*drug effects
MH  - Hypnotics and Sedatives/*pharmacology
MH  - Mice, Inbred C57BL
MH  - Propofol/*pharmacology
MH  - Receptors, GABA-A/*metabolism
PMC - PMC6786414
OTO - NOTNLM
OT  - GABAA receptors
OT  - anesthesia
OT  - basal forebrain
OT  - cholinergic neurons
OT  - picrotoxin
OT  - propofol
COIS- The authors declare no competing interests.
EDAT- 2018/10/28 06:00
MHDA- 2019/10/16 06:00
PMCR- 2020/06/01
CRDT- 2018/10/28 06:00
PHST- 2018/03/13 00:00 [received]
PHST- 2018/08/31 00:00 [accepted]
PHST- 2018/10/28 06:00 [pubmed]
PHST- 2019/10/16 06:00 [medline]
PHST- 2018/10/28 06:00 [entrez]
PHST- 2020/06/01 00:00 [pmc-release]
AID - 10.1038/s41401-018-0168-6 [pii]
AID - 168 [pii]
AID - 10.1038/s41401-018-0168-6 [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2019 Jun;40(6):755-761. doi: 10.1038/s41401-018-0168-6. Epub 
      2018 Oct 26.