PMID- 30368056
OWN - NLM
STAT- MEDLINE
DCOM- 20190129
LR  - 20190129
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 526
DP  - 2019 Jan 2
TI  - Long-range PCR and high-throughput sequencing of Ostreid herpesvirus 1 indicate 
      high genetic diversity and complex evolution process.
PG  - 81-90
LID - S0042-6822(18)30305-2 [pii]
LID - 10.1016/j.virol.2018.09.026 [doi]
AB  - Ostreid herpesvirus 1 (OsHV-1) is an important pathogen associated with mass 
      mortalities of cultivated marine mollusks worldwide. Since no cell line allows 
      OsHV-1 replication in vitro, it is difficult to isolate enough high-purity viral 
      DNA for High-Throughput Sequencing (HTS). We developed an efficient approach for 
      the enrichment of OsHV-1 DNA for HTS with long-range PCR. Twenty-three primer 
      pairs were designed to cover 99.3% of the reference genome, and their 
      performances were examined on ten OsHV-1 infected samples. Amplicon mixtures from 
      six successfully amplified samples were sequenced with Illumina platform, and one 
      of them (ZK0118) was also sequenced with the PacBio platform. PacBio reads were 
      assembled into 2 scaffolds compared to 9-68 scaffolds assembled from the Illumina 
      reads. Genomic comparison confirmed high genetic diversity among OsHV-1 variants. 
      Phylogenetic analysis revealed that OsHV-1 evolution was mainly impacted by its 
      host species rather than spatial segregation.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Bai, Chang-Ming
AU  - Bai CM
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture; 
      Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology; Qingdao Key Laboratory of 
      Mariculture Epidemiology and Biosecurity; Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
FAU - Morga, Benjamin
AU  - Morga B
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer (IFREMER), 
      Laboratoire de Genetique et Pathologie (LGP), Avenue de Mus de Loup, 17390 La 
      Tremblade, France.
FAU - Rosani, Umberto
AU  - Rosani U
AD  - Department of Biology, University of Padua, Padua, Italy.
FAU - Shi, Jie
AU  - Shi J
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture; 
      Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology; Qingdao Key Laboratory of 
      Mariculture Epidemiology and Biosecurity; Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
FAU - Li, Chen
AU  - Li C
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture; 
      Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology; Qingdao Key Laboratory of 
      Mariculture Epidemiology and Biosecurity; Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
FAU - Xin, Lu-Sheng
AU  - Xin LS
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture; 
      Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology; Qingdao Key Laboratory of 
      Mariculture Epidemiology and Biosecurity; Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
FAU - Wang, Chong-Ming
AU  - Wang CM
AD  - Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture; 
      Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology; Qingdao Key Laboratory of 
      Mariculture Epidemiology and Biosecurity; Yellow Sea Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China. 
      Electronic address: wangcm@ysfri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181024
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - DNA Viruses/*classification/*genetics
MH  - DNA, Viral/genetics
MH  - *Evolution, Molecular
MH  - *Genetic Variation
MH  - Genome, Viral/genetics
MH  - *High-Throughput Nucleotide Sequencing
MH  - Host Specificity
MH  - Phylogeny
MH  - *Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Diversity
OT  - Evolution
OT  - High-throughput sequencing
OT  - Long-range PCR
OT  - OsHV-1
EDAT- 2018/10/28 06:00
MHDA- 2019/01/30 06:00
CRDT- 2018/10/28 06:00
PHST- 2018/07/19 00:00 [received]
PHST- 2018/09/29 00:00 [revised]
PHST- 2018/09/29 00:00 [accepted]
PHST- 2018/10/28 06:00 [pubmed]
PHST- 2019/01/30 06:00 [medline]
PHST- 2018/10/28 06:00 [entrez]
AID - S0042-6822(18)30305-2 [pii]
AID - 10.1016/j.virol.2018.09.026 [doi]
PST - ppublish
SO  - Virology. 2019 Jan 2;526:81-90. doi: 10.1016/j.virol.2018.09.026. Epub 2018 Oct 
      24.