PMID- 30368732
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20200225
IS  - 1439-0973 (Electronic)
IS  - 0300-8126 (Linking)
VI  - 47
IP  - 2
DP  - 2019 Apr
TI  - Fluorescent in situ hybridization can be used as a complementary assay for the 
      diagnosis of Tropheryma whipplei infection.
PG  - 317-321
LID - 10.1007/s15010-018-1243-0 [doi]
AB  - BACKGROUND: Immunohistochemistry and Periodic acid-Schiff (PAS) staining have 
      been routinely used for the diagnosis of Whipple's disease (WD). However, these 
      methods present limitations. As a result, the last years, Fluorescence in situ 
      hybridization (FISH) has been increasingly used as a complementary tool for the 
      diagnosis of WD from various tissue samples. CASE REPORT: In this study, we 
      visualized, by FISH, Tropheryma whipplei within macrophages of a lymph node from 
      a patient with WD. Moreover, we report in this study a patient with a pulmonary 
      biopsy compatible with WD by PAS, immunostaining and FISH, although the specific 
      molecular assays for T. whipplei were negative. Sequencing analysis of the 16S 
      rDNA revealed a T. whipplei-related species with unknown classification. 
      CONCLUSION: FISH can be a valuable method for the detection of Tropheryma species 
      in formalin-fixed paraffin-embedded tissues. FISH cannot replace the other 
      already approved diagnostic techniques for WD, it can be used as a complementary 
      tool and can provide supplementary information in a relatively short time.
FAU - Prudent, Elsa
AU  - Prudent E
AD  - Aix Marseille Universite, IRD, APHM, MEPHI, IHU-Mediterranee Infection, 19-21 
      Boulevard Jean Moulin, 13005, Marseille, France.
FAU - Le Guenno, Guillaume
AU  - Le Guenno G
AD  - Department of Internal Medicine, Hopital Estaing, Centre, Hospitaliser 
      Universitaire, Clermont-Ferrand, France.
FAU - Jonckheere, Stijn
AU  - Jonckheere S
AD  - Laboratory of Clinical Microbiology, OLV Hospital Aalst, Aalst, Belgium.
FAU - Vankeerberghen, Anne
AU  - Vankeerberghen A
AD  - Laboratory of Clinical Microbiology, OLV Hospital Aalst, Aalst, Belgium.
FAU - Lepidi, Hubert
AU  - Lepidi H
AD  - Aix Marseille Universite, IRD, APHM, MEPHI, IHU-Mediterranee Infection, 19-21 
      Boulevard Jean Moulin, 13005, Marseille, France.
FAU - Angelakis, Emmanouil
AU  - Angelakis E
AD  - Aix Marseille Universite, IRD, APHM, VITROME, IHU-Mediterranee Infection, 19-21 
      Boulevard Jean Moulin, 13005, Marseille, France. e.angelakis@hotmail.com.
AD  - Laboratory of Medical Microbiology, Hellenic Pasteur Institute, Athens, Greece. 
      e.angelakis@hotmail.com.
FAU - Raoult, Didier
AU  - Raoult D
AD  - Aix Marseille Universite, IRD, APHM, MEPHI, IHU-Mediterranee Infection, 19-21 
      Boulevard Jean Moulin, 13005, Marseille, France.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20181027
PL  - Germany
TA  - Infection
JT  - Infection
JID - 0365307
SB  - IM
MH  - Adult
MH  - Aged
MH  - Belgium
MH  - Biopsy
MH  - Female
MH  - France
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymph Nodes/pathology
MH  - Macrophages/pathology
MH  - Tropheryma/classification/*isolation & purification
MH  - Whipple Disease/*diagnosis/microbiology
OTO - NOTNLM
OT  - Fluorescent in situ hybridization
OT  - Lymphadenopathy
OT  - Respiratory infection
OT  - Tropheryma whipplei
OT  - Whipple's disease
EDAT- 2018/10/29 06:00
MHDA- 2019/07/06 06:00
CRDT- 2018/10/29 06:00
PHST- 2018/06/21 00:00 [received]
PHST- 2018/10/22 00:00 [accepted]
PHST- 2018/10/29 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PHST- 2018/10/29 06:00 [entrez]
AID - 10.1007/s15010-018-1243-0 [pii]
AID - 10.1007/s15010-018-1243-0 [doi]
PST - ppublish
SO  - Infection. 2019 Apr;47(2):317-321. doi: 10.1007/s15010-018-1243-0. Epub 2018 Oct 
      27.