PMID- 30369271
OWN - NLM
STAT- MEDLINE
DCOM- 20200501
LR  - 20200501
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 44
IP  - 3
DP  - 2019 Mar
TI  - Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells 
      Treated with Homocysteine.
PG  - 287-293
LID - 10.1080/02713683.2018.1542005 [doi]
AB  - PURPOSE: To characterize the global profile of circular RNAs (circRNAs) and their 
      differential expression levels in homocysteine (Hcy)-treated ARPE-19 cells, a 
      line of human retinal pigment epithelial (RPE) cells. MATERIALS AND METHODS: We 
      treated ARPE-19 cells with and without Hcy to investigate the influence of Hcy on 
      circRNA expression levels using dedicated human circRNA microarrays. RESULTS: A 
      total of 12,233 circRNAs were identified out of them 54 were differentially 
      expressed (17 were down-regulated, and 37 were up-regulated) with a fold change 
      >2.0 (p < 0.05) in Hcy-treated versus untreated cells. CONCLUSIONS: To our 
      knowledge, this is the first report profiling circRNAs in human RPE cells 
      post-Hcy treatment mimicking hyperhomocysteinemic (HHcy) conditions that 
      negatively affect retinal biology and vision. These findings are of potential 
      clinical significance as they will help understand Hcy metabolism and 
      HHcy-mediated diseases and identify potential diagnostic and therapeutic targets 
      for eye diseases that are caused by elevated Hcy concentrations.
FAU - Singh, Mahavir
AU  - Singh M
AD  - a Eye and Vision Science Laboratory, Department of Physiology , University of 
      Louisville School of Medicine , Louisville , Kentucky , USA.
AD  - b Department of Physiology , University of Louisville School of Medicine , 
      Louisville , Kentucky , USA.
FAU - George, Akash K
AU  - George AK
AD  - a Eye and Vision Science Laboratory, Department of Physiology , University of 
      Louisville School of Medicine , Louisville , Kentucky , USA.
AD  - b Department of Physiology , University of Louisville School of Medicine , 
      Louisville , Kentucky , USA.
FAU - Homme, Rubens Petit
AU  - Homme RP
AD  - a Eye and Vision Science Laboratory, Department of Physiology , University of 
      Louisville School of Medicine , Louisville , Kentucky , USA.
AD  - b Department of Physiology , University of Louisville School of Medicine , 
      Louisville , Kentucky , USA.
FAU - Majumder, Avisek
AU  - Majumder A
AD  - b Department of Physiology , University of Louisville School of Medicine , 
      Louisville , Kentucky , USA.
FAU - Laha, Anwesha
AU  - Laha A
AD  - b Department of Physiology , University of Louisville School of Medicine , 
      Louisville , Kentucky , USA.
FAU - Sandhu, Harpal S
AU  - Sandhu HS
AD  - c Department of Ophthalmology and Visual Sciences , University of Louisville 
      School of Medicine , Louisville , Kentucky , USA.
AD  - d Kentucky Lions Eye Center, University of Louisville School of Medicine , 
      Louisville , Kentucky , USA.
FAU - Tyagi, Suresh C
AU  - Tyagi SC
AD  - b Department of Physiology , University of Louisville School of Medicine , 
      Louisville , Kentucky , USA.
LA  - eng
GR  - U01 HL074815/HL/NHLBI NIH HHS/United States
GR  - R01 HL107640/HL/NHLBI NIH HHS/United States
GR  - R01 AR067667/AR/NIAMS NIH HHS/United States
GR  - R01 AR071789/AR/NIAMS NIH HHS/United States
GR  - R01 DK104653/DK/NIDDK NIH HHS/United States
GR  - R01 HL139047/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20181205
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0LVT1QZ0BA (Homocysteine)
SB  - IM
MH  - Cell Line
MH  - Epigenomics
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*physiology
MH  - Homocysteine/*pharmacology
MH  - Humans
MH  - MicroRNAs/genetics
MH  - RNA, Circular/*genetics
MH  - Retinal Pigment Epithelium/*drug effects/metabolism
MH  - Up-Regulation
PMC - PMC6709669
MID - NIHMS1027186
OTO - NOTNLM
OT  - CircRNA
OT  - epigenetics
OT  - eye diseases
OT  - inflammation
OT  - retina
COIS- Disclosure statement No potential conflict of interest was reported by the 
      authors.
EDAT- 2018/10/30 06:00
MHDA- 2020/05/02 06:00
PMCR- 2019/08/26
CRDT- 2018/10/30 06:00
PHST- 2018/10/30 06:00 [pubmed]
PHST- 2020/05/02 06:00 [medline]
PHST- 2018/10/30 06:00 [entrez]
PHST- 2019/08/26 00:00 [pmc-release]
AID - 10.1080/02713683.2018.1542005 [doi]
PST - ppublish
SO  - Curr Eye Res. 2019 Mar;44(3):287-293. doi: 10.1080/02713683.2018.1542005. Epub 
      2018 Dec 5.