PMID- 3037152
OWN - NLM
STAT- MEDLINE
DCOM- 19870824
LR  - 20071114
IS  - 0027-8874 (Print)
IS  - 0027-8874 (Linking)
VI  - 79
IP  - 2
DP  - 1987 Aug
TI  - Mammary cancer stages in BALB/cV mice: mouse mammary tumor virus expression and 
      virus-host interactions.
PG  - 323-35
AB  - A unique subline of BALB/c mice, designated "BALB/cV," exhibits an intermediate 
      mammary tumor incidence (47%) and harbors a distinct milk-transmitted mouse 
      mammary tumor virus (MMTV). Virus expression and virus-host interactions were 
      examined during the different stages of mammary tumorigenesis (normal, 
      preneoplastic, and neoplastic) in the BALB/cV system. Protein immunoblot analyses 
      established the presence of correctly processed (BALB/cV)MMTV structural proteins 
      in all types of BALB/cV mammary tissues. Competition enzyme-linked immunosorbent 
      assays demonstrated that cells from each biologic phenotype were capable of 
      supporting high levels of (BALB/cV)MMTV protein expression. However, mammary 
      epithelial cells that spontaneously underwent the inappropriate pathway of 
      squamous metaplasia did not contain detectable levels of (BALB/cV)MMTV structural 
      proteins. Iodination experiments revealed the presence of a 68K env-related 
      protein on the surface of BALB/cV mammary cells. Nevertheless, sera from 40 mice 
      bearing BALB/cV-positive mammary tissues did not contain detectable levels of 
      anti-env antibodies. Metabolic labeling experiments showed that the half-life of 
      transformation-related, host cell protein p53 (approximately equal to 60 min) in 
      the distinct BALB/cV mammary cell populations was similar to that reported for 
      normal mouse 3T3 cells. It appears that p53 is not stabilized by protein 
      interactions involving any MMTV-encoded or MMTV-induced protein in mammary tumor 
      cells. These characteristics of the BALB/cV system are compatible with the 
      hypothesis that MMTV is only one of two or more cooperating factors required to 
      mediate complete mammary transformation.
FAU - Slagle, B L
AU  - Slagle BL
FAU - Medina, D
AU  - Medina D
FAU - Butel, J S
AU  - Butel JS
LA  - eng
GR  - CA-09197/CA/NCI NIH HHS/United States
GR  - CA-25215/CA/NCI NIH HHS/United States
GR  - CA-33369/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Natl Cancer Inst
JT  - Journal of the National Cancer Institute
JID - 7503089
RN  - 0 (Antibodies, Viral)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Cell Differentiation
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Mammary Glands, Animal/microbiology
MH  - Mammary Neoplasms, Experimental/metabolism/*microbiology
MH  - Mammary Tumor Virus, Mouse/immunology
MH  - Metaplasia/microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasm Proteins/analysis
MH  - Neoplasm Staging
MH  - Phenotype
MH  - Phosphoproteins/analysis
MH  - Tumor Suppressor Protein p53
MH  - Viral Envelope Proteins/immunology
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
PST - ppublish
SO  - J Natl Cancer Inst. 1987 Aug;79(2):323-35.