PMID- 30372852
OWN - NLM
STAT- MEDLINE
DCOM- 20190311
LR  - 20190311
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 108
DP  - 2018 Dec
TI  - Red ginseng-derived saponin fraction suppresses the obesity-induced inflammatory 
      responses via Nrf2-HO-1 pathway in adipocyte-macrophage co-culture system.
PG  - 1507-1516
LID - S0753-3322(18)35266-1 [pii]
LID - 10.1016/j.biopha.2018.09.169 [doi]
AB  - The aim of this study was to investigate the effect of saponin fraction (SF) from 
      red ginseng on obesity-induced inflammatory response in a co-culture system of 
      3T3-L1 and RAW264.7 cells. HPLC analysis showed that SF contains more than 50% 
      ginsenosides, and Rb1 was the most abundant ginsenoside [135.31 mug/mg (extract)]. 
      The production of nitric oxide and cytokines, induced by adipocyte-conditioned 
      medium (3T3-CM), was significantly decreased by SF. SF (100 mug/mL) suppressed the 
      abundance of tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant 
      protein-1 (MCP-1), and interleukin-6 (IL-6) by 78%, 40%, and 22%, respectively. 
      This SF-mediated reduction in inflammatory cytokines was due to the suppression 
      of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, 
      alpha (IkappaBalpha) phosphorylation, and translocation of nuclear factor 
      kappa-light-chain-enhancer of activated B cells (NF-kappaB) into the nucleus. SF also 
      regulated adipokine expression in adipocytes, which were stimulated by 
      macrophage-conditioned medium (RAW-CM); adiponectin expression was upregulated (> 
      2-fold), while resistin was downregulated (40%). In the contact system of 
      adipocytes and macrophages, SF significantly decreases MCP-1 (37%) and IL-6 (25%) 
      production. In the transwell system, SF (100 mug/mL) significantly increased the 
      abundance of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its 
      target protein, hemoxygenase-1 (HO-1) by 1.5 approximately 3.5-fold and 2.8 approximately 3.6-fold, 
      respectively, thus increasing Nrf2 translocation into nucleus. However, 
      SF-mediated inhibitory effect on the release of IL-6 and MCP-1 cytokines was 
      reversed in the Nrf2 or HO-1 knockdown condition. This result indicated that 
      SF-mediated inhibition of obesity-induced inflammation was dependent on Nrf2 
      activation.
CI  - Copyright (c) 2018 Elsevier Masson SAS. All rights reserved.
FAU - Kim, Chae Young
AU  - Kim CY
AD  - Department of Public Health Sciences, Korea University, Seoul 07249, Republic of 
      Korea.
FAU - Kang, Bobin
AU  - Kang B
AD  - Department of Public Health Sciences, Korea University, Seoul 07249, Republic of 
      Korea.
FAU - Suh, Hyung Joo
AU  - Suh HJ
AD  - Department of Public Health Sciences, Korea University, Seoul 07249, Republic of 
      Korea.
FAU - Choi, Hyeon-Son
AU  - Choi HS
AD  - Department of Food Science and Technology, College of Natural Science, Seoul 
      Women's University, Seoul 139-774, Republic of Korea. Electronic address: 
      hschoi@swu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20181009
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Inflammation Mediators)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Saponins)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/drug effects/*metabolism
MH  - Animals
MH  - Coculture Techniques
MH  - Dose-Response Relationship, Drug
MH  - Heme Oxygenase-1/antagonists & inhibitors/*metabolism
MH  - Inflammation Mediators/antagonists & inhibitors/metabolism
MH  - Macrophages/drug effects/*metabolism
MH  - Membrane Proteins/antagonists & inhibitors/*metabolism
MH  - Mice
MH  - NF-E2-Related Factor 2/antagonists & inhibitors/*metabolism
MH  - Obesity/drug therapy/*metabolism
MH  - Panax
MH  - RAW 264.7 Cells
MH  - Saponins/isolation & purification/pharmacology/*therapeutic use
MH  - Signal Transduction/drug effects/physiology
OTO - NOTNLM
OT  - Co-culture
OT  - Nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-hemoxygenase-1 (HO-1) 
      signaling pathway
OT  - Obesity-induced inflammation
OT  - Saponin fraction
EDAT- 2018/10/31 06:00
MHDA- 2019/03/12 06:00
CRDT- 2018/10/31 06:00
PHST- 2018/07/29 00:00 [received]
PHST- 2018/09/27 00:00 [revised]
PHST- 2018/09/28 00:00 [accepted]
PHST- 2018/10/31 06:00 [pubmed]
PHST- 2019/03/12 06:00 [medline]
PHST- 2018/10/31 06:00 [entrez]
AID - S0753-3322(18)35266-1 [pii]
AID - 10.1016/j.biopha.2018.09.169 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2018 Dec;108:1507-1516. doi: 10.1016/j.biopha.2018.09.169. 
      Epub 2018 Oct 9.