PMID- 30373234
OWN - NLM
STAT- MEDLINE
DCOM- 20181231
LR  - 20181231
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 23
IP  - 11
DP  - 2018 Oct 27
TI  - Shape Effects of Peptide Amphiphile Micelles for Targeting Monocytes.
LID - 10.3390/molecules23112786 [doi]
LID - 2786
AB  - Peptide amphiphile micelles (PAMs) are a nanoparticle platform that have gained 
      popularity for their targeting versatility in a wide range of disease models. An 
      important aspect of micelle design is considering the type of hydrophobic moiety 
      used to synthesize the PAM, which can act as a contributing factor regarding 
      their morphology and targeting capabilities. To delineate and compare the 
      characteristics of spherical and cylindrical micelles, we incorporated the 
      monocyte-targeting chemokine, monocyte chemoattractant protein-1 (MCP-1), into 
      our micelles (MCP-1 PAMs). We report that both shapes of nanoparticles were 
      biocompatible with monocytes and enhanced the secondary structure of the MCP-1 
      peptide, thereby improving the ability of the micelles to mimic the native MCP-1 
      protein structure. As a result, both shapes of MCP-1 PAMs effectively targeted 
      monocytes in an in vitro binding assay with murine monocytes. Interestingly, 
      cylindrical PAMs showed a greater ability to attract monocytes compared to 
      spherical PAMs in a chemotaxis assay. However, the surface area, the multivalent 
      display of peptides, and the zeta potential of PAMs may also influence their 
      biomimetic properties. Herein, we introduce variations in the methods of PAM 
      synthesis and discuss the differences in PAM characteristics that can impact the 
      recruitment of monocytes, a process associated with disease and cancer 
      progression.
FAU - Joo, Johan
AU  - Joo J
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, CA 90089, USA. johanjoo@usc.edu.
FAU - Poon, Christopher
AU  - Poon C
AUID- ORCID: 0000-0002-9797-7162
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, CA 90089, USA. poonc@usc.edu.
FAU - Yoo, Sang Pil
AU  - Yoo SP
AD  - Institute for Molecular Engineering, University of Chicago, 5747 South Ellis 
      Avenue, Chicago, IL 60637, USA. sangpilyoo@mednet.ucla.edu.
AD  - Current Affiliation: Medical Scientist Training Program, David Geffen School of 
      Medicine, University of California, Los Angeles, CA 90096, USA. 
      sangpilyoo@mednet.ucla.edu.
FAU - Chung, Eun Ji
AU  - Chung EJ
AUID- ORCID: 0000-0002-7726-5555
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, CA 90089, USA. eunchung@usc.edu.
AD  - Mork Family Department of Chemical Engineering and Materials Science, University 
      of Southern California, Los Angeles, CA 90089, USA. eunchung@usc.edu.
AD  - Division of Nephrology and Hypertension, Department of Medicine, Keck School of 
      Medicine, University of Southern California, Los Angeles, CA 90033, USA. 
      eunchung@usc.edu.
AD  - Norris Comprehensive Cancer Center, University of Southern California, Los 
      Angeles, CA 90033, USA. eunchung@usc.edu.
AD  - Department of Stem Cell Biology and Regenerative Medicine, University of Southern 
      California, Los Angeles, CA 90033, USA. eunchung@usc.edu.
LA  - eng
GR  - R00 HL124279/HL/NHLBI NIH HHS/United States
GR  - R00HL124279/National Heart, Lung, and Blood Institute/
GR  - 00347-00001/L. K. Whittier Foundation/
PT  - Journal Article
DEP - 20181027
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Chemotactic Factors)
RN  - 0 (Micelles)
RN  - 0 (Peptides)
RN  - 0 (Surface-Active Agents)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemotactic Factors/chemistry/pharmacology
MH  - Mice
MH  - *Micelles
MH  - Monocytes/*drug effects
MH  - Nanoparticles/chemistry/ultrastructure
MH  - Peptides/*chemistry/*pharmacology
MH  - Surface-Active Agents/*chemistry/*pharmacology
PMC - PMC6278295
OTO - NOTNLM
OT  - monocyte targeting
OT  - monocytes
OT  - nanoparticle shape
OT  - peptide amphiphile micelles
COIS- The authors declare no conflict of interest.
EDAT- 2018/10/31 06:00
MHDA- 2019/01/01 06:00
PMCR- 2018/10/27
CRDT- 2018/10/31 06:00
PHST- 2018/09/24 00:00 [received]
PHST- 2018/10/23 00:00 [revised]
PHST- 2018/10/25 00:00 [accepted]
PHST- 2018/10/31 06:00 [entrez]
PHST- 2018/10/31 06:00 [pubmed]
PHST- 2019/01/01 06:00 [medline]
PHST- 2018/10/27 00:00 [pmc-release]
AID - molecules23112786 [pii]
AID - molecules-23-02786 [pii]
AID - 10.3390/molecules23112786 [doi]
PST - epublish
SO  - Molecules. 2018 Oct 27;23(11):2786. doi: 10.3390/molecules23112786.