PMID- 3037324
OWN - NLM
STAT- MEDLINE
DCOM- 19870724
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 7
IP  - 4
DP  - 1987 Apr
TI  - Glucocorticoid-regulated compartmentalization of cell surface-associated 
      glycoproteins in rat hepatoma cells: evidence for an independent response that 
      requires receptor function and de novo RNA synthesis.
PG  - 1508-17
AB  - The role of glucocorticoid hormones in the compartmentalization of cell 
      surface-associated mouse mammary tumor virus (MMTV) glycoproteins was examined in 
      M1.54, a cloned line of MMTV-infected rat hepatoma tissue culture cells. The 
      expression of cellular [2-3H]mannose-labeled and cell surface 125I-labeled MMTV 
      glycoproteins was examined throughout a time course of exposure to dexamethasone, 
      a synthetic glucocorticoid. Posttranslational localization of cell surface MMTV 
      glycoproteins required 6 h of exposure to hormone and occurred approximately 4 h 
      after their initial production in an intracellular fraction. This regulated 
      localization to the cell surface correlated with glucocorticoid receptor 
      occupancy and was inhibited by exposure to RU 38486, a powerful antagonist of 
      glucocorticoid-mediated responses. Cell surface immunoprecipitation demonstrated 
      that actinomycin D, an inhibitor of de novo RNA synthesis, prevented regulated 
      expression of cell surface viral glycoproteins, suggesting that newly synthesized 
      cellular components mediate this process. The localization of cell surface MMTV 
      glycoproteins appeared normal in a transcriptional variant (CR1) that produces 
      basal levels of MMTV RNA and glycoprotein precursors in the presence of 
      dexamethasone. Thus, regulated compartmentalization of viral glycoproteins is not 
      an obligate consequence of a critical precursor concentration. Taken together, 
      our results suggest that posttranslational trafficking of cell surface-destined 
      MMTV glycoproteins resulted from an independent glucocorticoid hormone response 
      that required receptor function and de novo RNA synthesis.
FAU - Haffar, O K
AU  - Haffar OK
FAU - Vallerga, A K
AU  - Vallerga AK
FAU - Marenda, S A
AU  - Marenda SA
FAU - Witchel, H J
AU  - Witchel HJ
FAU - Firestone, G L
AU  - Firestone GL
LA  - eng
GR  - CA 09041/CA/NCI NIH HHS/United States
GR  - CA 35547/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Antigens, Viral)
RN  - 0 (Glycoproteins)
RN  - 0 (Retroviridae Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*genetics
MH  - Cell Line
MH  - Dexamethasone/*pharmacology
MH  - Genes/drug effects
MH  - Genes, Viral/drug effects
MH  - Glycoproteins/biosynthesis/*genetics
MH  - Kinetics
MH  - Liver Neoplasms, Experimental/*genetics
MH  - Mammary Tumor Virus, Mouse/drug effects/*genetics
MH  - Protein Processing, Post-Translational/drug effects
MH  - Rats
MH  - Retroviridae Proteins/biosynthesis/*genetics
MH  - *Transcription, Genetic
PMC - PMC365239
EDAT- 1987/04/01 00:00
MHDA- 1987/04/01 00:01
PMCR- 1987/04/01
CRDT- 1987/04/01 00:00
PHST- 1987/04/01 00:00 [pubmed]
PHST- 1987/04/01 00:01 [medline]
PHST- 1987/04/01 00:00 [entrez]
PHST- 1987/04/01 00:00 [pmc-release]
AID - 10.1128/mcb.7.4.1508-1517.1987 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1987 Apr;7(4):1508-17. doi: 10.1128/mcb.7.4.1508-1517.1987.