PMID- 30374362
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 9
DP  - 2018
TI  - Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio 
      Rootstocks in vitro Culture.
PG  - 1474
LID - 10.3389/fpls.2018.01474 [doi]
LID - 1474
AB  - The aim of this study was to determine the effects of Murashige and Skoog (MS) 
      salts on optimal growth of two pistachio rootstocks, P. vera cv. "Ghazvini" and 
      "UCB1" using design of experiments (DOE) and artificial intelligence (AI) tools. 
      MS medium with 14 macro-and micro-elements was used as base point and its 
      concentration varied from 0 to 5 x MS concentrations. Design of experiments (DOE) 
      software was used to generate a five-dimensional design space by categorizing MS 
      salts into five independent factors (NH(4)NO(3), KNO(3), mesos, micros and iron), 
      reducing the experimental design space from 3,125 to just 29 treatments. Typical 
      plant growth parameters such as shoot quality (SQ), proliferation rate (PR), 
      shoot length (SL), and some physiological disorders including shoot-tip necrosis 
      (STN) and callus formation at the base of explants (BC) were evaluated for each 
      treatment. The results were successfully modeled using neurofuzzy logic software. 
      The model delivered new insights, by different sets of "IF-THEN" rules, 
      pinpointing the key role of some ion interactions ( SO42- x Cl(-), K(+) x SO42- x 
      EDTA(-), and Fe(2+) x Cu(2+) x NO3- ) for SQ, PR, and SL, whilst physiological 
      disorders (STN and BC) were governed mainly by independent ions as Fe(2+) and 
      EDTA(-), respectively. In our opinion, the methodology and results obtained in 
      this study is extremely useful to understand the effect of mineral nutrients on 
      pistachio in vitro culture, through discovering new complex interactions among 
      macro-and micro-elements which can be implemented to design new media of plant 
      tissue culture and improve healthy plant micropropagation for any plant species.
FAU - Nezami-Alanagh, Esmaeil
AU  - Nezami-Alanagh E
AD  - Applied Plant and Soil Biology, Faculty of Biology, University of Vigo, Vigo, 
      Spain.
AD  - Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam 
      Khomeini International University, Qazvin, Iran.
FAU - Garoosi, Ghasem-Ali
AU  - Garoosi GA
AD  - Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam 
      Khomeini International University, Qazvin, Iran.
FAU - Landin, Mariana
AU  - Landin M
AD  - Department of Pharmacology, Pharmacy and Pharmaceutical Technology, Faculty of 
      Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain.
FAU - Gallego, Pedro Pablo
AU  - Gallego PP
AD  - Applied Plant and Soil Biology, Faculty of Biology, University of Vigo, Vigo, 
      Spain.
LA  - eng
PT  - Journal Article
DEP - 20181015
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC6196285
OTO - NOTNLM
OT  - DOE
OT  - health shoots
OT  - micropropagation
OT  - neurofuzzy logic
OT  - physiological disorders
OT  - pistachio rootstock
EDAT- 2018/10/31 06:00
MHDA- 2018/10/31 06:01
PMCR- 2018/01/01
CRDT- 2018/10/31 06:00
PHST- 2018/06/19 00:00 [received]
PHST- 2018/09/20 00:00 [accepted]
PHST- 2018/10/31 06:00 [entrez]
PHST- 2018/10/31 06:00 [pubmed]
PHST- 2018/10/31 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2018.01474 [doi]
PST - epublish
SO  - Front Plant Sci. 2018 Oct 15;9:1474. doi: 10.3389/fpls.2018.01474. eCollection 
      2018.