PMID- 30374697
OWN - NLM
STAT- MEDLINE
DCOM- 20190531
LR  - 20210109
IS  - 1861-0293 (Electronic)
IS  - 1340-3443 (Linking)
VI  - 73
IP  - 1
DP  - 2019 Jan
TI  - Establishment of widely applicable DNA extraction methods to identify the origins 
      of crude drugs derived from animals using molecular techniques.
PG  - 173-178
LID - 10.1007/s11418-018-1261-3 [doi]
AB  - We established widely applicable DNA extraction methods to identify the origins 
      of crude drugs derived from animals. Twenty-one samples including 17 kinds of 
      crude drug derived from animals were examined. DNA was extracted from most of the 
      crude drugs by adjustment of the QIAamp((R)) DNA Mini Kit. DNA extraction was 
      performed successfully using phenol to remove impurities after applying a 
      proteinase treatment. DNA extraction was performed successfully by 
      decalcification treatment using ethylenediaminetetraacetic acid (EDTA), before 
      applying the proteinase treatment for crude drugs having high calcium content, 
      such as those from oyster shell and cuttlefish bone. DNA could not be extracted 
      from sea-ear shell using the EDTA decalcification treatment, but was extracted 
      successfully using a TBONE EX KIT. The mitochondrial 16S ribosomal RNA (rRNA) 
      gene region was amplified, and Basic Local Alignment Search Tool (BLAST) analysis 
      was performed after sequencing. Polymerase chain reaction (PCR) products of 
      approximately 600 bp in length were obtained from all samples except donkey glue, 
      one of the two seahorses, and longgu. Drug origins were determined in all samples 
      by sequence analysis based on the BLAST results, and match rates were >97 %. 
      Moreover, 16 samples had a match rate >99 %. Our DNA extraction methods were 
      widely applicable to evaluation of many crude drugs derived from animals, and 
      proved very useful for identifying the origins of such drugs.
FAU - Nakanishi, Hiroaki
AU  - Nakanishi H
AD  - Department of Forensic Medicine, Juntendo University School of Medicine, 2-1-1, 
      Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan. hnakani@juntendo.ac.jp.
FAU - Yoneyama, Katsumi
AU  - Yoneyama K
AD  - Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, 
      Moroyama, Saitama, 350-0495, Japan.
FAU - Hayashizaki, Yoshie
AU  - Hayashizaki Y
AD  - Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, 
      Moroyama, Saitama, 350-0495, Japan.
FAU - Hara, Masaaki
AU  - Hara M
AD  - Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, 
      Moroyama, Saitama, 350-0495, Japan.
FAU - Takada, Aya
AU  - Takada A
AD  - Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, 
      Moroyama, Saitama, 350-0495, Japan.
FAU - Saito, Kazuyuki
AU  - Saito K
AD  - Department of Forensic Medicine, Juntendo University School of Medicine, 2-1-1, 
      Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan.
LA  - eng
GR  - JP16K08304/Japan Society for the Promotion of Science (JP)/
PT  - Journal Article
DEP - 20181029
PL  - Japan
TA  - J Nat Med
JT  - Journal of natural medicines
JID - 101518405
RN  - 0 (Complex Mixtures)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Pharmaceutical Preparations)
SB  - IM
MH  - Animals
MH  - Complex Mixtures/*metabolism
MH  - DNA, Bacterial/*metabolism
MH  - Pharmaceutical Preparations/*chemistry
OTO - NOTNLM
OT  - BLAST analysis
OT  - Crude drugs
OT  - DNA extraction
OT  - Mitochondrial 16S rRNA gene
OT  - Origin identification
EDAT- 2018/10/31 06:00
MHDA- 2019/06/01 06:00
CRDT- 2018/10/31 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2018/10/17 00:00 [accepted]
PHST- 2018/10/31 06:00 [pubmed]
PHST- 2019/06/01 06:00 [medline]
PHST- 2018/10/31 06:00 [entrez]
AID - 10.1007/s11418-018-1261-3 [pii]
AID - 10.1007/s11418-018-1261-3 [doi]
PST - ppublish
SO  - J Nat Med. 2019 Jan;73(1):173-178. doi: 10.1007/s11418-018-1261-3. Epub 2018 Oct 
      29.