PMID- 30376072 OWN - NLM STAT- MEDLINE DCOM- 20190306 LR - 20200220 IS - 1525-3171 (Electronic) IS - 0032-5791 (Linking) VI - 98 IP - 2 DP - 2019 Feb 1 TI - Molecular characterization, pathogenicity, and protection efficacy analysis of 2 wild-type lentogenic class I Newcastle disease viruses from chickens in China. PG - 602-612 LID - 10.3382/ps/pey471 [doi] AB - In this study, 2 wild-type Newcastle disease viruses (NDVs), designated as CK/GX/65/15 and CK/GX/26/15, were isolated from asymptomatic chickens in Guangxi province, China. They were identified as lentogenic NDV with mean death time (MDT) above 90 and intracerebral pathogenicity index (ICPI) below 0.7. The results of complete genome sequence analysis show that the 2 NDV strains are members of class I genotype 3 with the length 15,198 nt, which followed the "rule of six" and the order 3'-NP-P-M-F-HN-L-5'. In addition, 8 amino acid substitutions were identified in the functional domains of fusion protein (F) of CK/GX/65/15 and 9 in CK/GX/26/15, whose amino acid sequences of F protein cleavage site are 112E-R-Q-E-R-L117. The isolates were found to be apathogenic in specific pathogen free (SPF) chickens and ducks without morbidity or mortality. Furthermore, the protection study shows that isolates can provide the same effective protection against a major NDV virulent strain in China (class II genotype VII) as the commercial vaccine LaSota. Moreover, vaccination with isolates reduced number of chickens shedding virus compared to those vaccinated with LaSota. In conclusion, 2 wild-type NDV strains exhibited fine protection efficacy against genotype VII NDV in poultry and can be considered as candidate vaccines against NDV. CI - (c) 2018 Poultry Science Association Inc. FAU - Wu, Che AU - Wu C AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. FAU - Hu, Jinzhi AU - Hu J AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. FAU - Ci, Xiaotong AU - Ci X AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. FAU - Nie, Yu AU - Nie Y AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. FAU - Chen, Dekui AU - Chen D AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. FAU - Zhang, Xinheng AU - Zhang X AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. AD - Guangdong Animal Virus Vector Vaccine Engineering Research Center, Guangzhou 510640, PR China. FAU - Chen, Weiguo AU - Chen W AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, PR China. AD - Guangdong Animal Virus Vector Vaccine Engineering Research Center, Guangzhou 510640, PR China. FAU - Lin, Wencheng AU - Lin W AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. AD - South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, PR China. AD - Guangdong Animal Virus Vector Vaccine Engineering Research Center, Guangzhou 510640, PR China. FAU - Xie, Qingmei AU - Xie Q AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China. AD - South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, PR China. AD - Guangdong Animal Virus Vector Vaccine Engineering Research Center, Guangzhou 510640, PR China. LA - eng PT - Journal Article PL - England TA - Poult Sci JT - Poultry science JID - 0401150 RN - 0 (Viral Fusion Proteins) SB - IM MH - Animals MH - *Chickens MH - China MH - *Ducks MH - Newcastle Disease/immunology/*virology MH - Newcastle disease virus/classification/*pathogenicity/*physiology MH - Phylogeny MH - Poultry Diseases/immunology/*virology MH - Sequence Analysis, Protein/veterinary MH - Viral Fusion Proteins/immunology MH - Virulence OTO - NOTNLM OT - class I NDV OT - molecular characteristic OT - pathogenicity OT - protection efficacy EDAT- 2018/10/31 06:00 MHDA- 2019/03/07 06:00 CRDT- 2018/10/31 06:00 PHST- 2018/05/23 00:00 [received] PHST- 2018/10/16 00:00 [accepted] PHST- 2018/10/31 06:00 [pubmed] PHST- 2019/03/07 06:00 [medline] PHST- 2018/10/31 06:00 [entrez] AID - S0032-5791(19)30530-9 [pii] AID - 10.3382/ps/pey471 [doi] PST - ppublish SO - Poult Sci. 2019 Feb 1;98(2):602-612. doi: 10.3382/ps/pey471.