PMID- 30378377
OWN - NLM
STAT- MEDLINE
DCOM- 20181126
LR  - 20181126
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 36
IP  - 11
DP  - 2018 Nov 1
TI  - [Simultaneous determination of 14 nutrients in vegetable oils by ultra high 
      performance liquid chromatography-tandem mass spectrometry].
PG  - 1140-1146
LID - 10.3724/SP.J.1123.2018.07013 [doi]
AB  - A method for the simultaneous determination of four tocopherols, four 
      tocotrienols, four phytosterols, beta-carotene and squalene by ultra high 
      performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was 
      developed. After saponification, the samples were extracted with petroleum ether, 
      and the extract solution was concentrated by rotary evaporation. The residue was 
      dried under a N(2) atmosphere and dissolved with methanol. The 14 nutrients were 
      separated on a Poroshell 120 PFP column (150 mmx3.0 mm, 2.7 mum) by gradient 
      elution with 0.1% (v/v) formic acid aqueous solution and methanol containing 
      0.1%(v/v) formic acid as the mobile phases at a flow rate of 0.3 mL/min. The 
      detection was performed using an atmospheric pressure chemical ionization 
      (APCI)-mass spectrometer with selective reaction monitoring (SRM) in positive 
      mode. The results were linear in the range of 0.05-10.0 mg/L for the 14 
      nutrients, and the correlation coefficients (r) were no less than 0.9971. The 
      average recoveries of the 14 nutrients in the vegetable oil samples ranged from 
      80.7% to 100.5% at three spiked levels, and the relative standard deviations 
      (RSDs) were less than 6.0%. The limits of detection (LODs) ranged from 0.01 to 
      0.30 mug/g. The limits of quantification (LOQs) were in the range of 0.04-1.00 
      mug/g. The proposed method is sensitive, accurate, fast and stable, and is 
      suitable for the simultaneous determination of the 14 nutrients in vegetable oil 
      samples.
FAU - Peng, Zumao
AU  - Peng Z
AD  - Shenzhen Academy of Metrology and Quality Inspection, National Nutrition Food 
      Testing Center(Guangdong), Shenzhen 518109, China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Shenzhen Academy of Metrology and Quality Inspection, National Nutrition Food 
      Testing Center(Guangdong), Shenzhen 518109, China.
FAU - Deng, Mengya
AU  - Deng M
AD  - Shenzhen Academy of Metrology and Quality Inspection, National Nutrition Food 
      Testing Center(Guangdong), Shenzhen 518109, China.
FAU - Zhang, Xieguang
AU  - Zhang X
AD  - Shenzhen Academy of Metrology and Quality Inspection, National Nutrition Food 
      Testing Center(Guangdong), Shenzhen 518109, China.
FAU - Zheng, Yuhui
AU  - Zheng Y
AD  - Shenzhen Academy of Metrology and Quality Inspection, National Nutrition Food 
      Testing Center(Guangdong), Shenzhen 518109, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Plant Oils)
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - Limit of Detection
MH  - Plant Oils/*analysis
MH  - *Tandem Mass Spectrometry
MH  - Vegetables/*chemistry
OTO - NOTNLM
OT  - phytosterols
OT  - squalene
OT  - ultra high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS)
OT  - vegetable oil
OT  - vitamin E
OT  - beta-carotene
EDAT- 2018/11/01 06:00
MHDA- 2018/11/27 06:00
CRDT- 2018/11/01 06:00
PHST- 2018/11/01 06:00 [entrez]
PHST- 2018/11/01 06:00 [pubmed]
PHST- 2018/11/27 06:00 [medline]
AID - 10.3724/SP.J.1123.2018.07013 [doi]
PST - ppublish
SO  - Se Pu. 2018 Nov 1;36(11):1140-1146. doi: 10.3724/SP.J.1123.2018.07013.