PMID- 30378560
OWN - NLM
STAT- MEDLINE
DCOM- 20190508
LR  - 20190508
IS  - 2310-6972 (Print)
IS  - 2310-6905 (Linking)
VI  - 64
IP  - 5
DP  - 2018 Sep
TI  - [Neutrophils as a source of factors that increase the length of the inflammatory 
      phase of wound healing in patients with type 2 diabetes mellitus].
PG  - 433-438
LID - 10.18097/PBMC20186405433 [doi]
AB  - Oxidative stress and neutrophil activation leading to an increase in 
      myeloperoxidase (MPO), elastase and neutrophil extracellular trap (NET) levels in 
      blood are considered as pathogenic mechanisms responsible for the development of 
      extremity damage in people with type 2 diabetes mellitus (T2DM). The aim of this 
      study was to analyze the relationship between factors, associated with neutrophil 
      activation, and the length of the initial phase of wound healing (the 
      inflammatory phase) in T2DM patients. Patients were divided retrospectively into 
      three groups depending on the damage extent: group 1 (wound on toe) < group 2 
      (wound on foot) < group 3 (wound on lower leg). Compared to the control group 
      (healthy volunteers), T2DM patients at admission to hospital had significantly 
      (p<0.05) increased levels of blood glucose and glycated hemoglobin (groups 1-3), 
      ESR (groups 1 and 3), blood neutrophil count (groups 2 and 3), plasma MPO 
      concentration (groups 1-3) and blood NET concentration (group 3) and decreased 
      levels of plasma thiols (groups 1-3) and erythrocyte glutathione peroxidase 
      activity (groups 2 and 3). The length of hospital stay after surgical procedures 
      corresponded to the length of the inflammatory phase of the wound healing process 
      and correlated with the number of blood neutrophils in patients before surgery 
      (r=0.72, p<0.05). Leukocytic intoxication index depended on wound area (r=0.59, 
      p<0.05), and it was significantly higher for groups 2 and 3 compared to the 
      control group and group 1. The neutrophil count before surgery in T2DM patients 
      with damage in the lower extremities correlated with the length of the 
      inflammatory phase of wound healing. The correlation found can be attributed to 
      an increase in extracellular MPO and NETs, which, in its turn, results from the 
      activation and degranulation of neutrophils and netosis. Thus, the duration of 
      the inflammatory phase of wound healing depends on specific aspects of systemic 
      inflammation increasing oxidative/halogenative stress and intoxication.
FAU - Mikhalchik, E V
AU  - Mikhalchik EV
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Maximov, D I
AU  - Maximov DI
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Ostrovsky, E M
AU  - Ostrovsky EM
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Yaskevich, A V
AU  - Yaskevich AV
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Vlasova, I I
AU  - Vlasova II
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Vakhrusheva, T V
AU  - Vakhrusheva TV
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Basyreva, L Yu
AU  - Basyreva LY
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Gusev, A A
AU  - Gusev AA
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Kostevich, V A
AU  - Kostevich VA
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia; Institute of Experimental Medicine, Saint-Petersburg, Russia.
FAU - Gorbunov, N P
AU  - Gorbunov NP
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia; State Research Institute of Highly Pure Biopreparations, 
      Saint-Petersburg, Russia.
FAU - Sokolov, A V
AU  - Sokolov AV
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia; Institute of Experimental Medicine, Saint-Petersburg, Russia.
FAU - Panasenko, O M
AU  - Panasenko OM
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
FAU - Gusev, S A
AU  - Gusev SA
AD  - Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, 
      Russia.
LA  - rus
PT  - Journal Article
TT  - Neitrofily kak istochnik faktorov, uvelichivaiushchikh prodolzhitel'nost' fazy 
      vospaleniia ranevogo protsessa u bol'nykh sakharnym diabetom 2-go tipa.
PL  - Russia (Federation)
TA  - Biomed Khim
JT  - Biomeditsinskaia khimiia
JID - 101196966
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - *Diabetes Mellitus, Type 2
MH  - Extracellular Traps
MH  - Humans
MH  - *Neutrophils
MH  - Peroxidase
MH  - Retrospective Studies
MH  - Wound Healing
OTO - NOTNLM
OT  - blood neutrophils
OT  - diabetes mellitus
OT  - diabetic foot
OT  - myeloperoxidase
OT  - neutrophil extracellular traps
OT  - oxidative/halogenative stress
EDAT- 2018/11/01 06:00
MHDA- 2019/05/09 06:00
CRDT- 2018/11/01 06:00
PHST- 2018/11/01 06:00 [entrez]
PHST- 2018/11/01 06:00 [pubmed]
PHST- 2019/05/09 06:00 [medline]
AID - 10.18097/PBMC20186405433 [doi]
PST - ppublish
SO  - Biomed Khim. 2018 Sep;64(5):433-438. doi: 10.18097/PBMC20186405433.