PMID- 30379219
OWN - NLM
STAT- MEDLINE
DCOM- 20190416
LR  - 20220629
IS  - 1980-5322 (Electronic)
IS  - 1807-5932 (Print)
IS  - 1807-5932 (Linking)
VI  - 73
DP  - 2018 Oct 29
TI  - alpha-Linolenic acid prevents hepatic steatosis and improves glucose tolerance in 
      mice fed a high-fat diet.
PG  - e150
LID - 10.6061/clinics/2018/e150 [doi]
LID - e150
AB  - OBJECTIVES: Dietary omega-3 fatty acids have been efficacious in decreasing serum 
      cholesterol levels and reducing the risk of cardiovascular disease. However, the 
      metabolic and molecular changes induced by the omega-3 fatty acid alpha-linolenic 
      acid (ALA), which is found in linseed oil, are not fully understood. In this 
      study, we showed a correlation between ALA and insulin resistance, inflammation 
      and endoplasmic reticulum stress (ERS). METHODS: We studied 40 male mice 
      (C57/BL6) divided into 4 groups: a control (C) group, a control + omega-3/ALA 
      (CA) group, a high-fat diet (HFD) (H) group and a high-fat diet + omega-3/ALA 
      (HA) group. For 8 weeks, the animals in the H and HA groups were fed a high-fat 
      (60%) diet, while the animals in the C and CA groups received regular chow. The 
      diets of the CA and HA groups were supplemented with 10% lyophilized ALA. 
      RESULTS: ALA supplementation improved glucose tolerance and reduced insulin 
      resistance, as measured by intraperitoneal glucose tolerance tests and the 
      homeostasis model assessment for insulin resistance, respectively. In addition, 
      ALA reduced hepatic steatosis and modified the standard fat concentration in the 
      liver of animals fed an HFD. Dietary ALA supplementation reduced the serum levels 
      of interleukin 6 (IL-6), interleukin 1 beta (IL-1beta) and monocyte chemoattractant 
      protein-1 (MCP-1), increased the expression of important chaperones such as 
      binding immunoglobulin protein (BIP) and heat shock protein 70 (HSP70) and 
      reduced the expression of C/EBP-homologous protein (CHOP) and X-box binding 
      protein 1 (XBP1) in hepatic tissues, suggesting an ERS adaptation in response to 
      ALA supplementation. CONCLUSIONS: Dietary ALA supplementation is effective in 
      preventing hepatic steatosis; is associated with a reduction in insulin 
      resistance, inflammation and ERS; and represents an alternative for improving 
      liver function and obtaining metabolic benefits.
FAU - Goncalves, Natalia Bonissi
AU  - Goncalves NB
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Bannitz, Rafael Ferraz
AU  - Bannitz RF
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Silva, Bruna Ramos
AU  - Silva BR
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Becari, Danielle Duran
AU  - Becari DD
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Poloni, Carolina
AU  - Poloni C
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Gomes, Patricia Moreira
AU  - Gomes PM
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Foss, Milton Cesar
AU  - Foss MC
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Foss-Freitas, Maria Cristina
AU  - Foss-Freitas MC
AD  - Departamento de Medicina, Divisao de Endocrinologia e Metabolismo, Faculdade de 
      Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
LA  - eng
PT  - Journal Article
DEP - 20181029
PL  - United States
TA  - Clinics (Sao Paulo)
JT  - Clinics (Sao Paulo, Brazil)
JID - 101244734
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0RBV727H71 (alpha-Linolenic Acid)
SB  - IM
MH  - Animals
MH  - *Diet, High-Fat
MH  - Dietary Supplements
MH  - Endoplasmic Reticulum Stress/drug effects
MH  - Fatty Acids, Omega-3/*administration & dosage/pharmacology
MH  - Fatty Liver/*prevention & control
MH  - Glucose Tolerance Test
MH  - Inflammation/*prevention & control
MH  - *Insulin Resistance
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - alpha-Linolenic Acid/*administration & dosage/pharmacology
PMC - PMC6201146
COIS- No potential conflict of interest was reported.
EDAT- 2018/11/01 06:00
MHDA- 2019/04/17 06:00
PMCR- 2018/01/01
CRDT- 2018/11/01 06:00
PHST- 2018/02/23 00:00 [received]
PHST- 2018/05/28 00:00 [accepted]
PHST- 2018/11/01 06:00 [entrez]
PHST- 2018/11/01 06:00 [pubmed]
PHST- 2019/04/17 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - S1807-5932(22)00520-8 [pii]
AID - cln_73p1 [pii]
AID - 10.6061/clinics/2018/e150 [doi]
PST - epublish
SO  - Clinics (Sao Paulo). 2018 Oct 29;73:e150. doi: 10.6061/clinics/2018/e150.