PMID- 30381591
OWN - NLM
STAT- MEDLINE
DCOM- 20190226
LR  - 20220321
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 131
IP  - 21
DP  - 2018 Nov 5
TI  - Overexpression of Dendritic Cell-Specific Intercellular Adhesion 
      Molecule-3-Grabbing Nonintegrin in Dendritic Cells Protecting against 
      Aspergillosis.
PG  - 2575-2582
LID - 10.4103/0366-6999.244103 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) play an important role in host defense against 
      pathogen infection. DC-specific intercellular adhesion molecule-3-grabbing 
      nonintegrin (SIGN) is a group II C-type lectin receptor and specifically 
      expressed on the surface of DCs. This study aimed to determine whether DC-SIGN 
      affects intracellular signaling activation, Th1/Th2 imbalance and aspergillus 
      immune evasion in aspergillus infection, and explore the application of 
      DC-SIGN-modified DCs in immunotherapy. METHODS: DCs were first obtained from the 
      mononuclear cells of peripheral blood. The interferon (IFN)-gamma and dexamethasone 
      (Dex) were used to stimulate DCs. The expression of DC-SIGN, Th1 and Th2 
      cytokines, and the capacity of DCs in stimulating T cells proliferation and 
      phagocytosis, and nuclear factor (NF)-kappaB activation were analyzed. In addition, 
      adenovirus expression vector Ad-DC-SIGN was generated to transfect DCs. Mannan 
      was used to block DC-SIGN signaling for confirming the involvement of DC-SIGN 
      function in Aspergillus fumigatus (Af)-induced DCs maturation. The unpaired, 
      two-tailed Student's t-test was used in the comparisons between two groups. 
      RESULTS: Exogenous IFN-gamma could activate Af-induced DCs and promote the Th0 cells 
      toward Th1 profile (interleukin [IL]-12 in IFN-gamma/Af group: 50.96 +/- 4.38 pg/ml; 
      control/Af group: 29.70 +/- 2.00 pg/ml, t = 10.815, P < 0.001). On the other hand, 
      Dex inhibited the secretion of Th2 cytokines (IL-10 in Dex/Af group: 5.27 +/- 0.85 
      pg/ml; control/Af group: 15.14 +/- 1.40 pg/ml, t = 14.761, P < 0.001)), and 
      successfully caused immunosuppression. After transfection with Ad-DC-SIGN, DCs 
      have improved phagocytosis (phagocytosis rates in Ad-DC-SIGN group: 74.0% +/- 3.4%; 
      control group: 64.7% +/- 6.8%, t = 3.104, P = 0.013). There was more Th1 cytokine 
      secreted in the Af-induced DC-SIGN modified DCs (IL-12 in Ad-DC-SIGN/Af group: 
      471.98 +/- 166.31 pg/ml; control/Af group: 33.35 +/- 5.98 pg/ml, t = 6.456, P = 
      0.001), correlated to the enhanced NF-kappaB activation. CONCLUSION: Overexpressing 
      DC-SIGN in DCs had a protective function on aspergillosis.
FAU - Li, Li-Yang
AU  - Li LY
AD  - Department of Pulmonary Medicine, Shanghai Institute of Respiratory Diseases, 
      Zhongshan Hospital, Fudan University, Shanghai 200032, China.
FAU - Zhang, Hao-Ru
AU  - Zhang HR
AD  - Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, 
      Biochemistry and Molecular Biology of Hebei Province, College of Life Science, 
      Hebei Normal University, Shijiazhuang, Hebei 050024, China.
FAU - Jiang, Zhi-Long
AU  - Jiang ZL
AD  - Department of Pulmonary Medicine, Shanghai Institute of Respiratory Diseases, 
      Zhongshan Hospital, Fudan University, Shanghai 200032, China.
FAU - Chang, Yan-Zhong
AU  - Chang YZ
AD  - Laboratory of Molecular Iron Metabolism, The Key Laboratory of Animal Physiology, 
      Biochemistry and Molecular Biology of Hebei Province, College of Life Science, 
      Hebei Normal University, Shijiazhuang, Hebei 050024, China.
FAU - Shao, Chang-Zhou
AU  - Shao CZ
AD  - Department of Pulmonary Medicine, Shanghai Institute of Respiratory Diseases, 
      Zhongshan Hospital, Fudan University, Shanghai 200032, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Cell Surface)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Aspergillosis/immunology/*metabolism
MH  - Aspergillus fumigatus/pathogenicity
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism
MH  - Dexamethasone/pharmacology
MH  - Humans
MH  - Immunosuppression Therapy
MH  - Immunotherapy
MH  - Interferon-gamma/pharmacology
MH  - Lectins, C-Type/*metabolism
MH  - NF-kappa B/metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Th1 Cells/immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
PMC - PMC6213851
OTO - NOTNLM
OT  - Asepergillus fumigatus
OT  - Dendritic Cell-Specific Intercellular Adhesion Molecule-3-Grabbing Nonintegrin
OT  - Dendritic Cells
OT  - Immunity
COIS- There are no conflicts of interest
EDAT- 2018/11/02 06:00
MHDA- 2019/02/27 06:00
PMCR- 2018/11/05
CRDT- 2018/11/02 06:00
PHST- 2018/11/02 06:00 [entrez]
PHST- 2018/11/02 06:00 [pubmed]
PHST- 2019/02/27 06:00 [medline]
PHST- 2018/11/05 00:00 [pmc-release]
AID - ChinMedJ_2018_131_21_2575_244103 [pii]
AID - CMJ-131-2575 [pii]
AID - 10.4103/0366-6999.244103 [doi]
PST - ppublish
SO  - Chin Med J (Engl). 2018 Nov 5;131(21):2575-2582. doi: 10.4103/0366-6999.244103.