PMID- 30381735
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221118
IS  - 1011-2367 (Print)
IS  - 1976-5517 (Electronic)
IS  - 1011-2367 (Linking)
VI  - 32
IP  - 12
DP  - 2019 Dec 1
TI  - Population genetic variations of the MMP3 gene revealed hypoxia adaptation in 
      domesticated yaks (Bos grunniens).
PG  - 1801-1808
LID - 10.5713/ajas.17.0706 [doi]
AB  - OBJECTIVE: As an iconic symbol of Qinghai-Tibetan Plateau and of high altitude, 
      yak is subjected to hypoxic conditions that challenge aerobic metabolism. Matrix 
      metalloproteinases-3 (MMP3) is assumed to be a key target gene of 
      hypoxia-inducible factor-1alpha (HIF-1alpha) that function as a master regulator of the 
      cellular response to hypoxia. Therefore, the aim of this investigation was to 
      identify the DNA polymorphism of MMP3 gene in domestic yak and to explore its 
      possible association with high-altitude adaptation. METHODS: The 
      single-nucleotide polymorphisms (SNPs) genotyping and mutations scanning at the 
      MMP3 locus were conducted in total of 344 individuals from four domestic Chinese 
      yak breeds resident at different altitudes on the Qinghai-Tibetan Plateau, using 
      high-resolution melting analysis（HRM）and DNA sequencing techniques. RESULTS: The 
      novel of SNPs rs2381 A-->G and rs4331 C-->G were identified in intron Ⅴ and intron Ⅶ 
      of MMP3, respectively. Frequencies of the GG genotype and the G allele of SNP 
      rs2381 A-->G observed in high-altitude Pali yak were significantly higher than that 
      of the other yak breeds resident at middle or low altitude (p < 0.01). No 
      significant difference was mapped for SNP rs4331 C-->G in the yak population (p 
      > 0.05). Haplotype GC was the dominant among the 4 yak breeds, and Pearson 
      correlation analysis showed that the frequencies of GC was significantly lower in 
      GN (Ganan), DT (Datong) and WY (Tianzhu white) yaks compared with Pali yak (PL). 
      The two SNPs were in moderate linkage disequilibrium in high-altitude yaks (PL) 
      but not in middle-altitude (GN, DT) and low-altitude (WY) yaks. CONCLUSION: These 
      results indicate that MMP3 may have been subjected to positive selection in yak, 
      especially that the SNP rs2381 A-->G mutation and GC haplotypes might contribute to 
      adaptation for yak in high-altitude environments.
FAU - Ding, Xuezhi
AU  - Ding X
AD  - Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and 
      Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 
      730050, China.
FAU - Yang, Chao
AU  - Yang C
AD  - Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and 
      Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 
      730050, China.
AD  - CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, South -Central Experimental Station of Animal Nutrition 
      and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering 
      Research Center for Healthy Livestock and Poultry Production Institute of 
      Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China.
FAU - Bao, Pengjia
AU  - Bao P
AD  - Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and 
      Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 
      730050, China.
FAU - Wu, Xiaoyun
AU  - Wu X
AD  - Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and 
      Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 
      730050, China.
FAU - Pei, Jie
AU  - Pei J
AD  - Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and 
      Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 
      730050, China.
FAU - Yan, Ping
AU  - Yan P
AD  - Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and 
      Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 
      730050, China.
FAU - Guo, Xian
AU  - Guo X
AD  - Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and 
      Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 
      730050, China.
LA  - eng
PT  - Journal Article
DEP - 20181029
PL  - Korea (South)
TA  - Asian-Australas J Anim Sci
JT  - Asian-Australasian journal of animal sciences
JID - 9884245
PMC - PMC6819682
OTO - NOTNLM
OT  - Hypoxia Adaptation
OT  - Matrix metalloproteinases-3 (MMP3)
OT  - Polymorphism
OT  - Yak
COIS- CONFLICT OF INTEREST We certify that there is no conflict of interest with any 
      financial organization regarding the material discussed in the manuscript.
EDAT- 2018/11/02 06:00
MHDA- 2018/11/02 06:01
PMCR- 2019/12/01
CRDT- 2018/11/02 06:00
PHST- 2017/09/22 00:00 [received]
PHST- 2018/10/18 00:00 [accepted]
PHST- 2018/11/02 06:00 [entrez]
PHST- 2018/11/02 06:00 [pubmed]
PHST- 2018/11/02 06:01 [medline]
PHST- 2019/12/01 00:00 [pmc-release]
AID - ajas.17.0706 [pii]
AID - ajas-17-0706 [pii]
AID - 10.5713/ajas.17.0706 [doi]
PST - ppublish
SO  - Asian-Australas J Anim Sci. 2019 Dec 1;32(12):1801-1808. doi: 
      10.5713/ajas.17.0706. Epub 2018 Oct 29.