PMID- 30384816
OWN - NLM
STAT- MEDLINE
DCOM- 20190507
LR  - 20190507
IS  - 1544-2217 (Electronic)
IS  - 0300-9858 (Linking)
VI  - 56
IP  - 2
DP  - 2019 Mar
TI  - HER2 Amplification Status in Feline Mammary Carcinoma: A Tissue 
      Microarray-Fluorescence In Situ Hydridization-Based Study.
PG  - 230-238
LID - 10.1177/0300985818808531 [doi]
AB  - Human epidermal growth factor receptor 2 (HER2) is a tyrosine kinase receptor 
      overexpressed in a subset of breast cancer due to HER2 gene amplification. HER2 
      protein is expressed in feline mammary carcinomas, but little is known about its 
      cytogenetic alterations. The aim of this study was to evaluate HER2 gene 
      amplification status and its correlation with HER2 protein expression in feline 
      mammary carcinomas. Feline mammary carcinomas were retrospectively selected and 
      immunohistochemically (IHC) evaluated for HER2 protein expression. All the HER2 
      IHC-positive (3+) and equivocal (2+) cases and a subset of negative cases (0/1+) 
      were selected for fluorescence in situ hybridization (FISH). Dual-core tissue 
      microarrays were prepared for FISH. IHC and FISH were evaluated according to the 
      2013 American Society of Clinical Oncology/College of American Pathologists 
      guidelines. The study included 107 feline mammary carcinomas from 88 queens. HER2 
      protein expression was positive (3+) in 7 cases (6.5%), equivocal (2+) in 48 
      cases (45%), and negative (0/1+) in 52 cases (48.5%). HER2 status was 
      indeterminate in 8 feline mammary carcinomas (12%), amplified in 3 (4%), 
      equivocal in 4 (6%), and nonamplified in 53 (78%). HER2 gene amplification and 
      protein expression were significantly positively correlated ( R = 0.283; P < 
      .0001). HER2 gene is amplified in a subset of feline mammary carcinomas despite 
      the HER2 positive or equivocal protein expression, but it remains to be 
      determined if the HER2 amplification is a gene alteration that drives mammary 
      tumor carcinogenesis or only a bystander passenger mutation.
FAU - Muscatello, Luisa Vera
AU  - Muscatello LV
AUID- ORCID: 0000-0003-4653-0624
AD  - 1 Department of Veterinary Medical Sciences, University of Bologna, Bologna, 
      Italy.
AD  - These authors contributed equally to this work.
FAU - Di Oto, Enrico
AU  - Di Oto E
AD  - 2 Molecular Pathology-Anatomic Pathology Laboratory, Oncological Institute 
      "F.Addarii"-S. Orsola Hospital, Bologna, Italy.
AD  - These authors contributed equally to this work.
FAU - Sarli, Giuseppe
AU  - Sarli G
AD  - 1 Department of Veterinary Medical Sciences, University of Bologna, Bologna, 
      Italy.
FAU - Monti, Valentina
AU  - Monti V
AD  - 3 Istituto Nazionale Tumori, Milan, Italy.
FAU - Foschini, Maria Pia
AU  - Foschini MP
AD  - 4 Anatomic Pathology, Department of Biomedical and Neuromotor Sciences, 
      University of Bologna, Bologna, Italy.
FAU - Benazzi, Cinzia
AU  - Benazzi C
AD  - 1 Department of Veterinary Medical Sciences, University of Bologna, Bologna, 
      Italy.
FAU - Brunetti, Barbara
AU  - Brunetti B
AD  - 1 Department of Veterinary Medical Sciences, University of Bologna, Bologna, 
      Italy.
LA  - eng
PT  - Journal Article
DEP - 20181101
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Animals
MH  - Cat Diseases/*metabolism
MH  - Cats
MH  - Female
MH  - Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, erbB-2/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Mammary Glands, Animal/metabolism
MH  - Mammary Neoplasms, Animal/*metabolism
MH  - Receptor, ErbB-2/*metabolism
MH  - Retrospective Studies
MH  - Tissue Array Analysis/veterinary
OTO - NOTNLM
OT  - gene amplification
OT  - HER2 protein overexpression
OT  - cat
OT  - feline mammary carcinoma
OT  - fluorescence in situ hybridization
OT  - immunohistochemistry
OT  - mammary gland
OT  - tissue microarray
EDAT- 2018/11/06 06:00
MHDA- 2019/05/08 06:00
CRDT- 2018/11/03 06:00
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2019/05/08 06:00 [medline]
PHST- 2018/11/03 06:00 [entrez]
AID - 10.1177/0300985818808531 [doi]
PST - ppublish
SO  - Vet Pathol. 2019 Mar;56(2):230-238. doi: 10.1177/0300985818808531. Epub 2018 Nov 
      1.