PMID- 3038544
OWN - NLM
STAT- MEDLINE
DCOM- 19870917
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 166
IP  - 3
DP  - 1987 Aug 3
TI  - Human protein binding to DNA sequences surrounding the human T-cell lymphotropic 
      virus type-I long terminal repeat polyadenylation site.
PG  - 519-26
AB  - The long terminal repeats (LTRs) of RNA tumor viruses, including human T-cell 
      lymphotropic virus type I (HTLV-I), contain the control elements for expression 
      of viral genes. Sequence-specific LTR-DNA-binding proteins could regulate viral 
      functions. To search for such proteins we have used an in vitro non-denaturing 
      polyacrylamide gel assay, with restriction fragments of the HTLV-I LTR and 
      nuclear protein extracts from several HTLV-I-infected cell lines and an 
      uninfected T-cell line, H9. Four DNA-binding activities were observed, including 
      non-specific DNA-binding activity and at least two activities (forms I and II) 
      which bind specifically to a HinfI restriction fragment from nucleotides +181 to 
      +334 relative to the transcription start site. DNA-binding activities I and II 
      were partially resolved by ion-exchange chromatography and mapped by protection 
      experiments to two 10-20-bp blocks surrounding the polyadenylation site at +221. 
      Of the cell lines tested, form II was abundantly found in C10/MJ, and forms I and 
      IV were also found in C91/PL, C81-66-45, MT2 and H9 cells.
FAU - Levinger, L F
AU  - Levinger LF
FAU - Lautenberger, J A
AU  - Lautenberger JA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 24937-83-5 (Poly A)
SB  - IM
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Cell Line
MH  - Cell Nucleus/analysis
MH  - Chromatography, DEAE-Cellulose
MH  - DNA, Viral/*metabolism
MH  - DNA-Binding Proteins/*analysis
MH  - Deltaretrovirus/*genetics
MH  - Humans
MH  - Poly A
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1987/08/03 00:00
MHDA- 1987/08/03 00:01
CRDT- 1987/08/03 00:00
PHST- 1987/08/03 00:00 [pubmed]
PHST- 1987/08/03 00:01 [medline]
PHST- 1987/08/03 00:00 [entrez]
AID - 10.1111/j.1432-1033.1987.tb13544.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1987 Aug 3;166(3):519-26. doi: 10.1111/j.1432-1033.1987.tb13544.x.