PMID- 30387321
OWN - NLM
STAT- MEDLINE
DCOM- 20200415
LR  - 20210109
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 23
IP  - 1
DP  - 2019 Jan
TI  - Dynein axonemal intermediate chain 2 plays a role in gametogenesis by activation 
      of Stat3.
PG  - 417-425
LID - 10.1111/jcmm.13945 [doi]
AB  - We previously identified the mouse dynein axonemal intermediate chain 2 (Dnaic2) 
      gene. This gene expresses a component of the axonemal dynein complex that 
      functions in cilia or flagella. We found that overexpression of Dnaic2 results in 
      female subfertility and male infertility. In this study, we generated Dnaic2 
      knockdown (KD) mice and identified the potential regulatory mechanisms involved 
      in Dnaic2 function. For phenotype analysis, we found that body weight was lighter 
      and size was smaller in Dnaic2 KD mice than in wild-type mice. A total of 45% of 
      these Dnaic2 KD mice were infertile due to sperm abnormalities in males, or had 
      oocyte abnormalities and pathological changes in the tunica mucosa in the oviduct 
      of females. Moreover, Dnaic2 overexpression enhanced the expression of 
      proliferating cell nuclear antigen (PCNA) in the ovaries, which suggested that 
      Dnaic2 stimulated proliferation of cells in the ovaries. However, PCNA expression 
      in the testis of Dnaic2-overexpressed mice was lower than that in controls. 
      Additionally, the ratio of Bax/B-cell lymphoma-2(Bcl-2) in the testis of 
      Dnaic2-overexpressed mice was higher than that in controls, which suggested that 
      Dnaic2 inhibited cellular proliferation in the testis. To examine the molecular 
      action of Dnaic2, immunoprecipitation analysis was used and showed that Dnaic2 
      protein interacted with signal transducer and activator of transcription 3 
      (Stat3). Molecular modelling analysis showed that Dnaic2 bound with the linker 
      and SH2 domains of Stat3. Furthermore, overexpression of Dnaic2 promoted 
      phosphorylation of Stat3. In conclusion, our study suggests that Dnaic2 plays a 
      role in oogenesis and spermatogenesis by activation of Stat3.
CI  - (c) 2018 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Yang, Zhaojuan
AU  - Yang Z
AD  - Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders 
      (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, 
      Shanghai, China.
FAU - Xu, Bo
AU  - Xu B
AD  - Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders 
      (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, 
      Shanghai, China.
FAU - Hu, Xiaopeng
AU  - Hu X
AD  - Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders 
      (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, 
      Shanghai, China.
FAU - Yao, Xiaoying
AU  - Yao X
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
FAU - Tang, Yunhui
AU  - Tang Y
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
FAU - Qian, Cuifeng
AU  - Qian C
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
FAU - Wang, Shuzeng
AU  - Wang S
AD  - Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders 
      (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, 
      Shanghai, China.
FAU - Chen, Haifeng
AU  - Chen H
AD  - School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 
      Shanghai, China.
FAU - Bai, Xiaohui
AU  - Bai X
AD  - School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 
      Shanghai, China.
FAU - Wu, Ji
AU  - Wu J
AUID- ORCID: 0000-0002-6424-9783
AD  - Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders 
      (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, 
      Shanghai, China.
AD  - Key Laboratory of Fertility Preservation and Maintenance of Ministry of 
      Education, Ningxia Medical University, Yinchuan, China.
AD  - Shanghai Key Laboratory of Reproductive Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181101
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Dnaic2 protein, mouse)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, mouse)
RN  - EC 3.6.4.2 (Axonemal Dyneins)
SB  - IM
MH  - Animals
MH  - Axonemal Dyneins/*metabolism
MH  - Cell Line
MH  - Cell Proliferation/physiology
MH  - Cilia/metabolism/physiology
MH  - Female
MH  - Gametogenesis/*physiology
MH  - HEK293 Cells
MH  - Humans
MH  - Infertility, Male/metabolism/physiopathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NIH 3T3 Cells
MH  - Ovary/metabolism/physiology
MH  - STAT3 Transcription Factor/*metabolism
MH  - Spermatogenesis/physiology
MH  - Testis/metabolism
PMC - PMC6307815
OTO - NOTNLM
OT  - Dnaic2
OT  - Stat3
OT  - oogenesis
OT  - spermatogenesis
EDAT- 2018/11/06 06:00
MHDA- 2020/04/16 06:00
PMCR- 2019/01/01
CRDT- 2018/11/03 06:00
PHST- 2018/07/09 00:00 [received]
PHST- 2018/09/10 00:00 [accepted]
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2020/04/16 06:00 [medline]
PHST- 2018/11/03 06:00 [entrez]
PHST- 2019/01/01 00:00 [pmc-release]
AID - JCMM13945 [pii]
AID - 10.1111/jcmm.13945 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2019 Jan;23(1):417-425. doi: 10.1111/jcmm.13945. Epub 2018 Nov 1.