PMID- 30388631
OWN - NLM
STAT- MEDLINE
DCOM- 20190129
LR  - 20190129
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 526
DP  - 2019 Jan 2
TI  - Recombinant fiber-2 protein protects Muscovy ducks against duck adenovirus 3 
      (DAdV-3).
PG  - 99-104
LID - S0042-6822(18)30323-4 [pii]
LID - 10.1016/j.virol.2018.10.011 [doi]
AB  - As a novel duck adenovirus 3 (DAdV-3) infection caused significant economic 
      losses to the poultry industry in China, there is an urgent need to develop a 
      safe and effective vaccine. In the research, fiber-1 and fiber-2 proteins were 
      expressed and purified, respectively. To evaluate the immunogenicity of the two 
      recombinant proteins, we investigated the IgY antibodies and virus-neutralizing 
      antibodies in duck sera. The protective efficacy was evaluated by mortality, 
      virus shedding and histopathological examinations after challenged with the 
      DAdV-3. Results showed that the IgY antibody levels of the fiber-2 group was 
      significantly higher than that of the fiber-1 group and inactivated vaccine 
      group. Ducks vaccinated with fiber-2 group provided full protection with no 
      mortality, no virus shedding and no histological lesions, superior to other 
      groups. These results suggest that the fiber-2 protein can be an ideal candidate 
      for subunit vaccine against the disease.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Yin, Lijuan
AU  - Yin L
AD  - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., 
      Xinxing, Guangdong, PR China; State Key Laboratory of Biocontrol, School of Life 
      Sciences, Sun Yat-sen University, Guangzhou, PR China.
FAU - Chen, Li
AU  - Chen L
AD  - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., 
      Xinxing, Guangdong, PR China; State Key Laboratory of Biocontrol, School of Life 
      Sciences, Sun Yat-sen University, Guangzhou, PR China.
FAU - Luo, Yangyang
AU  - Luo Y
AD  - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., 
      Xinxing, Guangdong, PR China.
FAU - Lin, Limiao
AU  - Lin L
AD  - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., 
      Xinxing, Guangdong, PR China.
FAU - Li, Qunhui
AU  - Li Q
AD  - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., 
      Xinxing, Guangdong, PR China.
FAU - Peng, Peng
AU  - Peng P
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, PR China.
FAU - Du, Yunping
AU  - Du Y
AD  - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., 
      Xinxing, Guangdong, PR China; State Key Laboratory of Biocontrol, School of Life 
      Sciences, Sun Yat-sen University, Guangzhou, PR China.
FAU - Xu, Zhichao
AU  - Xu Z
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, PR China.
FAU - Xue, Chunyi
AU  - Xue C
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, PR China.
FAU - Cao, Yongchang
AU  - Cao Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, PR China.
FAU - Zhou, Qingfeng
AU  - Zhou Q
AD  - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd., 
      Xinxing, Guangdong, PR China. Electronic address: zhqf1012@163.com.
LA  - eng
PT  - Journal Article
DEP - 20181026
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adenoviridae Infections/pathology/prevention & control/*veterinary
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Aviadenovirus/genetics/*immunology
MH  - Ducks/*immunology
MH  - Immunoglobulins/blood
MH  - Poultry Diseases/pathology/*prevention & control
MH  - Recombinant Proteins/genetics/*immunology
MH  - Vaccines, Inactivated/administration & dosage/genetics/immunology
MH  - Viral Proteins/genetics/*immunology
MH  - Viral Vaccines/administration & dosage/genetics/*immunology
MH  - Virus Shedding
OTO - NOTNLM
OT  - DAdV-3
OT  - Fiber-1 protein
OT  - Fiber-2 protein
OT  - Immunogenicity
EDAT- 2018/11/06 06:00
MHDA- 2019/01/30 06:00
CRDT- 2018/11/03 06:00
PHST- 2018/09/01 00:00 [received]
PHST- 2018/10/11 00:00 [revised]
PHST- 2018/10/11 00:00 [accepted]
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2019/01/30 06:00 [medline]
PHST- 2018/11/03 06:00 [entrez]
AID - S0042-6822(18)30323-4 [pii]
AID - 10.1016/j.virol.2018.10.011 [doi]
PST - ppublish
SO  - Virology. 2019 Jan 2;526:99-104. doi: 10.1016/j.virol.2018.10.011. Epub 2018 Oct 
      26.