PMID- 30389164
OWN - NLM
STAT- MEDLINE
DCOM- 20190610
LR  - 20200225
IS  - 1879-3592 (Electronic)
IS  - 0027-5107 (Print)
IS  - 1383-5718 (Linking)
VI  - 836
IP  - Pt A
DP  - 2018 Dec
TI  - Development of an automatable micro-PCC biodosimetry assay for rapid 
      individualized risk assessment in large-scale radiological emergencies.
PG  - 65-71
LID - S1383-5718(18)30017-2 [pii]
LID - 10.1016/j.mrgentox.2018.05.013 [doi]
AB  - In radiation accidents and large-scale radiological emergencies, a fast and 
      reliable triage of individuals according to their degree of exposure is important 
      for accident management and identification of those who need medical assistance. 
      In this work, the applicability of cell-fusion-mediated premature chromosome 
      condensation (PCC) in G(0)-lymphocytes is examined for the development of a 
      rapid, minimally invasive and automatable micro-PCC assay, which requires blood 
      volumes of only 100 mul and can be performed in 96-well plates, towards risk 
      assessments and categorization of individuals based on dose estimates. 
      Chromosomal aberrations are visualized for dose-estimation analysis within two 
      hours, without the need of blood culturing for two days, as required by 
      conventional cytogenetics. The various steps of the standard-PCC procedure were 
      adapted and, for the first time, lymphocytes in blood volumes of 100 mul were 
      successfully fused with CHO-mitotics in 96-well plates of 2 ml/well. The plates 
      are advantageous for high-throughput analysis since the various steps required 
      are applied to all 96-wells simultaneously. Interestingly, the use of only 1.5 ml 
      hypotonic and Carnoy's fixative per well offers high quality PCC-images, and the 
      morphology of lymphocyte PCCs is identical to that obtained using the 
      conventional PCC-assay, which requires much larger blood volumes and 15 ml tubes. 
      For dose assessments, appropriate calibration curves were constructed and for PCC 
      analysis specialized software (MetaSystems) was used. The micro-PCC assay can be 
      combined with fluorescence in situ hybridization (FISH), using simultaneously 
      centromeric/telomeric (C/T) peptide nucleic acid (PNA) probes. This allows dose 
      assessments on the basis of accurate scoring of dicentric and centric ring 
      chromosomes in G(0)-lymphocyte PCCs, which is particularly helpful when further 
      evaluation into treatment-level categories of exposed individuals is needed. The 
      micro-PCC assay has significant advantages for early triage biodosimetry when 
      compared to other cytogenetic biodosimetry assays. It is rapid, cost-effective, 
      and could pave the way to its subsequent automation.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Pantelias, Antonio
AU  - Pantelias A
AD  - Laboratory of Health Physics, Radiobiology & Cytogenetics, Institute of Nuclear & 
      Radiological Sciences & Technology, Energy & Safety, National Centre for 
      Scientific Research "Demokritos", Athens, Greece.
FAU - Terzoudi, Georgia I
AU  - Terzoudi GI
AD  - Laboratory of Health Physics, Radiobiology & Cytogenetics, Institute of Nuclear & 
      Radiological Sciences & Technology, Energy & Safety, National Centre for 
      Scientific Research "Demokritos", Athens, Greece. Electronic address: 
      gterzoudi@rrp.demokritos.gr.
LA  - eng
GR  - U19 AI067773/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20180507
PL  - Netherlands
TA  - Mutat Res Genet Toxicol Environ Mutagen
JT  - Mutation research. Genetic toxicology and environmental mutagenesis
JID - 101632149
SB  - IM
MH  - Animals
MH  - Biological Assay/*methods
MH  - CHO Cells
MH  - Cell Fusion
MH  - Chromosome Aberrations/*radiation effects
MH  - Chromosomes, Human/*radiation effects
MH  - Cricetulus
MH  - Emergencies
MH  - Healthy Volunteers
MH  - Humans
MH  - Lymphocytes/*pathology/radiation effects
MH  - Radiation Dosage
MH  - Radioactive Hazard Release
MH  - Risk Assessment/*methods
PMC - PMC6486952
MID - NIHMS1526801
OTO - NOTNLM
OT  - Excess PCC fragments
OT  - High-throughput biodosimetry
OT  - Lymphocyte fusion
OT  - Micro-PCC assay
OT  - Premature chromosome condensation (PCC)
COIS- Conflict of Interest statement: The authors declare that there are no conflicts 
      of interest.
EDAT- 2018/11/06 06:00
MHDA- 2019/06/14 06:00
PMCR- 2019/12/01
CRDT- 2018/11/04 06:00
PHST- 2018/01/24 00:00 [received]
PHST- 2018/03/30 00:00 [revised]
PHST- 2018/05/07 00:00 [accepted]
PHST- 2018/11/04 06:00 [entrez]
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2019/12/01 00:00 [pmc-release]
AID - S1383-5718(18)30017-2 [pii]
AID - 10.1016/j.mrgentox.2018.05.013 [doi]
PST - ppublish
SO  - Mutat Res Genet Toxicol Environ Mutagen. 2018 Dec;836(Pt A):65-71. doi: 
      10.1016/j.mrgentox.2018.05.013. Epub 2018 May 7.