PMID- 30391037
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20231213
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1580
DP  - 2018 Dec 14
TI  - Development and validation of a method for quantification of two tobacco-specific 
      nitrosamines in indoor air.
PG  - 90-99
LID - S0021-9673(18)31307-4 [pii]
LID - 10.1016/j.chroma.2018.10.037 [doi]
AB  - A sensitive and accurate method for the quantification of 
      1'-Demethyl-1'-nitrosonicotine (NNN) and 
      4-(methylnitrosamino)-1-(3-Pyridyl)-1-butanone (NNK) in indoor air was developed 
      and validated. To this aim, a novel approach for the collection of two 
      tobacco-specific nitrosamines, using silica sorbent cartridges followed by 
      simplified sample preparation and isotope dilution liquid 
      chromatography/electrospray ionization tandem mass spectrometry, was applied. 
      This procedure led to a substantial improvement in terms of sensitivity and 
      sample throughput as compared with methods using conventional trapping. For the 
      validation, a matrix-based approach using an accuracy profile procedure was 
      selected. The evaluated matrices were background air samples, environmental 
      aerosols of a heat-not-burn tobacco product (Tobacco Heating System [THS] 2.2, 
      commercialized under the brand IQOS((R))), a rechargeable electronic cigarette 
      (Solaris((R))), and the environmental tobacco smoke (ETS) of a conventional 
      cigarette (Marlboro Gold((R))). The method showed excellent recoveries, 
      sensitivity, and precision. The limits of detection of the method for NNN and NNK 
      were 0.0108 ng/m(3) and 0.0136 ng/m(3), respectively. The calibration range of 
      the instrument spanned 0.2-60 ng/mL. The calculated lower working range limit 
      (LWRL) of the method for NNN was 0.126 ng/m(3), and the LWRL for NNK was 
      0.195 ng/m(3). The method was applied to evaluate surrogate environmental 
      aerosols generated using smoking machines. This model is reliable but gives a 
      large overestimation of the possible impact of THS 2.2 and e-cigarettes on indoor 
      air, because the retention of NNN and NNK in the body of the consumers is not 
      taken into account. As a consequence, the values reported do not reflect a 
      real-life setting. The contents of the two target compounds in the surrogate 
      environmental aerosols were 0.0830 +/- 0.0153 ng/m(3) of NNN and 
      0.0653 +/- 0.0138 ng/m(3) of NNK for THS 2.2, 0.0561 +/- 0.0296 ng/m(3) of NNN for 
      e-cigarettes, and 0.816 +/- 0.109 ng/m(3) of NNN and 4.13 +/- 1.04 ng/m(3) NNK for 
      cigarettes. These values correspond to 10% of the measured ETS concentration for 
      NNN in environmental aerosols of THS 2.2 and 7% for those of e-cigarettes. For 
      NNK, the value for the environmental aerosol of THS 2.2 was 2% of the ETS value.
CI  - Copyright (c) 2018 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 
      Neuchatel, Switzerland. Published by Elsevier B.V. All rights reserved.
FAU - Gomez Lueso, Maria
AU  - Gomez Lueso M
AD  - PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchatel, 
      Switzerland.
FAU - Mitova, Maya I
AU  - Mitova MI
AD  - PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchatel, 
      Switzerland. Electronic address: Maya.Mitova@pmi.com.
FAU - Mottier, Nicolas
AU  - Mottier N
AD  - PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchatel, 
      Switzerland; Service de la Consommation et des Affaires Veterinaires, Chemin des 
      Boveresses 155, 1066 Epalinges, Switzerland.
FAU - Schaller, Mathieu
AU  - Schaller M
AD  - PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchatel, 
      Switzerland.
FAU - Rotach, Michel
AU  - Rotach M
AD  - PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchatel, 
      Switzerland.
FAU - Goujon-Ginglinger, Catherine G
AU  - Goujon-Ginglinger CG
AD  - PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchatel, 
      Switzerland.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20181023
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Air Pollutants)
RN  - 0 (Carcinogens)
RN  - 0 (Nitrosamines)
SB  - IM
MH  - Air Pollutants/*analysis
MH  - Air Pollution, Indoor/*analysis
MH  - Carcinogens/analysis
MH  - Chromatography, Liquid
MH  - Electronic Nicotine Delivery Systems
MH  - Environmental Monitoring/*methods
MH  - Nitrosamines/*analysis
MH  - Smoking
MH  - Nicotiana/*chemistry
MH  - Tobacco Products/analysis
OTO - NOTNLM
OT  - Accuracy profile
OT  - Environmental aerosol
OT  - Tobacco heating system
OT  - Tobacco-specific nitrosamines
OT  - Validation
OT  - e-Cigarette
EDAT- 2018/11/06 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/11/05 06:00
PHST- 2018/07/13 00:00 [received]
PHST- 2018/09/28 00:00 [revised]
PHST- 2018/10/17 00:00 [accepted]
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/11/05 06:00 [entrez]
AID - S0021-9673(18)31307-4 [pii]
AID - 10.1016/j.chroma.2018.10.037 [doi]
PST - ppublish
SO  - J Chromatogr A. 2018 Dec 14;1580:90-99. doi: 10.1016/j.chroma.2018.10.037. Epub 
      2018 Oct 23.