PMID- 30392071
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20200225
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 42
IP  - 2
DP  - 2019 Apr
TI  - MiR-590-3p Attenuates Acute Kidney Injury by Inhibiting Tumor Necrosis Factor 
      Receptor-Associated Factor 6 in Septic Mice.
PG  - 637-649
LID - 10.1007/s10753-018-0921-5 [doi]
AB  - Previous studies have been indicated that tumor necrosis factor 
      receptor-associated factor 6 (TRAF6)-induced inflammation leads to acute kidney 
      injury (AKI). How microRNA (miR) contributes to this process is poorly defined. 
      The aim of this study was to investigate whether miR-590-3p regulated 
      lipopolysaccharide (LPS)-induced inflammatory response by inhibiting TRAF6. 
      LPS-induced septic mice were treated with adenovirus expressing miR-590-3p 
      (ad-miR-590-3p) via tail-vein injection. AKI was evaluated by examining serum 
      cystatin C (CysC), serum beta2-microglobulin (beta2-MG), and blood urea nitrogen (BUN). 
      The mRNA and protein levels were assayed by RT-qPCR and western blotting, 
      respectively. The proliferation of podocytes was monitored using the MTT assay. 
      Cell apoptosis was analyzed by flow cytometry. Survival outcomes in 
      ad-miR-590-3p-transfected septic mice were markedly improved compared with mice 
      with LPS-induced sepsis. Ad-miR-590-3p transfection significantly attenuated 
      LPS-induced AKI, which was reflected by an improved glomerular filtration rate 
      (GFR) as determined by measuring CysC, beta2-MG, and BUN. Moreover, we observed that 
      miR-590-3p was a novel regulator of TRAF6, binding to its 3'-untranslated regions 
      (3'-UTRs). In vitro, a miR-590-3p gain-of-function mutation blocked LPS-induced 
      podocyte growth inhibition and apoptosis, as well as overactivation of the 
      inflammatory response. miR-590-3p has the ability to suppress LPS-induced AKI and 
      podocyte apoptosis by targeting TRAF6. This might provide a novel strategy for 
      the treatment of LPS-induced renal injuries.
FAU - Ma, Jing
AU  - Ma J
AD  - Department of Emergency, Optics Valley hospital, Wuhan Third Hospital of Wuhan 
      University, No. 216 Guanshan Road, Wuhan, 430073, China.
FAU - Li, Yu-Tao
AU  - Li YT
AD  - Department of Emergency, Optics Valley hospital, Wuhan Third Hospital of Wuhan 
      University, No. 216 Guanshan Road, Wuhan, 430073, China.
FAU - Zhang, Shi-Xiong
AU  - Zhang SX
AD  - Department of Emergency, Optics Valley hospital, Wuhan Third Hospital of Wuhan 
      University, No. 216 Guanshan Road, Wuhan, 430073, China.
FAU - Fu, Shou-Zhi
AU  - Fu SZ
AD  - Department of Emergency, Optics Valley hospital, Wuhan Third Hospital of Wuhan 
      University, No. 216 Guanshan Road, Wuhan, 430073, China. sz_futh@aliyun.com.
FAU - Ye, Xian-Zhi
AU  - Ye XZ
AD  - Department of Emergency, Optics Valley hospital, Wuhan Third Hospital of Wuhan 
      University, No. 216 Guanshan Road, Wuhan, 430073, China. xianzhi_ye123@163.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MIRN590 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (TNF Receptor-Associated Factor 6)
MH  - Acute Kidney Injury/drug therapy/*prevention & control
MH  - Animals
MH  - Apoptosis
MH  - Inflammation/chemically induced
MH  - Lipopolysaccharides
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Podocytes/pathology
MH  - Sepsis/complications/*pathology
MH  - Survival Rate
MH  - TNF Receptor-Associated Factor 6/*antagonists & inhibitors
OTO - NOTNLM
OT  - AKI
OT  - TRAF6
OT  - apoptosis
OT  - inflammatory response
OT  - miR-590-3p
OT  - sepsis
EDAT- 2018/11/06 06:00
MHDA- 2019/08/03 06:00
CRDT- 2018/11/05 06:00
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
PHST- 2018/11/05 06:00 [entrez]
AID - 10.1007/s10753-018-0921-5 [pii]
AID - 10.1007/s10753-018-0921-5 [doi]
PST - ppublish
SO  - Inflammation. 2019 Apr;42(2):637-649. doi: 10.1007/s10753-018-0921-5.