PMID- 30393176
OWN - NLM
STAT- MEDLINE
DCOM- 20190322
LR  - 20190322
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 84
DP  - 2019 Jan
TI  - Molecular characterization of a new fish specific chemokine CXCL_F6 in large 
      yellow croaker (Larimichthys crocea) and its role in inflammatory response.
PG  - 787-794
LID - S1050-4648(18)30701-0 [pii]
LID - 10.1016/j.fsi.2018.10.068 [doi]
AB  - Chemokines are a superfamily of structurally related chemotactic cytokines 
      exerting significant roles in regulating cell migration and activation. 
      Currently, five subgroups of fish specific CXC chemokines, named CXCL_F1-CXCL_F5, 
      have been identified in teleost fish. However, understanding of the functions of 
      these fish specific CXC chemokines is still limited. Here, a new member of fish 
      specific CXC chemokines, LcCXCL_F6, was cloned from large yellow croaker 
      Larimichthys crocea. Its open reading frame (ORF) is 369 nucleotides long, 
      encoding a peptide of 122 amino acids (aa). The deduced LcCXCL_F6 protein 
      contains a 19-aa signal peptide and a 103-aa mature polypeptide, which has four 
      conserved cysteine residues (C(28), C(30), C(56), and C(72)), as found in other 
      known CXC chemokines. Phylogenetic analysis showed LcCXCL_F6 formed a separate 
      clade with sequences from other fish species, tentatively named CXCL_F6, distinct 
      from the clades formed by fish CXCL_F1-5 and mammalian CXC chemokines. The 
      LcCXCL_F6 transcripts were constitutively expressed in all examined tissues and 
      significantly up-regulated in the spleen and head kidney tissues by poly (I:C) 
      and Vibrio alginolyticus. Its transcripts were also detected in primary head 
      kidney leukocytes (HKLs), peripheral blood leucocytes (PBLs), and large yellow 
      croaker head kidney (LYCK) cell line, and significantly up-regulated by 
      poly(I:C), lipopolysaccharide (LPS), and peptidoglycan (PGN) in HKLs. Recombinant 
      LcCXCL_F6 protein (rLcCXCL_F6) could not only chemotactically attract 
      monocytes/macrophages and lymphocytes from PBLs, but also enhance NO release and 
      expression of proinflammatory cytokines (TNF-alpha, IL-1beta, and CXCL8) in 
      monocytes/macrophages. These results indicate that LcCXCL_F6 plays a role in 
      mediating the inflammatory response.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Mu, Yinnan
AU  - Mu Y
AD  - Institute of Oceanology, College of Animal Sciences, Fujian Agriculture and 
      Forestry University, Fuzhou, 350002, China.
FAU - Zhou, Shimin
AU  - Zhou S
AD  - Institute of Oceanology, College of Animal Sciences, Fujian Agriculture and 
      Forestry University, Fuzhou, 350002, China.
FAU - Ding, Ning
AU  - Ding N
AD  - Institute of Oceanology, College of Animal Sciences, Fujian Agriculture and 
      Forestry University, Fuzhou, 350002, China.
FAU - Ao, Jingqun
AU  - Ao J
AD  - Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, 
      State Oceanic Administration, Xiamen, 361005, China.
FAU - Chen, Xinhua
AU  - Chen X
AD  - Institute of Oceanology, College of Animal Sciences, Fujian Agriculture and 
      Forestry University, Fuzhou, 350002, China; Laboratory for Marine Biology and 
      Biotechnology, Qingdao National Laboratory for Marine Science and Technology, 
      Qingdao, China. Electronic address: chenxinhua@tio.org.cn.
LA  - eng
PT  - Journal Article
DEP - 20181026
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Chemokine CXCL6)
RN  - 0 (Fish Proteins)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chemokine CXCL6/chemistry/*genetics/*immunology
MH  - Fish Diseases/*immunology
MH  - Fish Proteins/chemistry/genetics/immunology
MH  - Gene Expression Profiling/veterinary
MH  - Gene Expression Regulation/*immunology
MH  - Immunity, Innate/*genetics
MH  - Perciformes/*genetics/*immunology
MH  - Phylogeny
MH  - Poly I-C/pharmacology
MH  - Sequence Alignment/veterinary
MH  - Vibrio Infections/immunology
MH  - Vibrio alginolyticus/physiology
OTO - NOTNLM
OT  - CXC chemokine
OT  - CXCL_F6
OT  - Chemotaxis
OT  - Inflammatory response
OT  - Large yellow croaker (Larimichthys crocea)
EDAT- 2018/11/06 06:00
MHDA- 2019/03/23 06:00
CRDT- 2018/11/06 06:00
PHST- 2018/07/24 00:00 [received]
PHST- 2018/09/13 00:00 [revised]
PHST- 2018/10/25 00:00 [accepted]
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2019/03/23 06:00 [medline]
PHST- 2018/11/06 06:00 [entrez]
AID - S1050-4648(18)30701-0 [pii]
AID - 10.1016/j.fsi.2018.10.068 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Jan;84:787-794. doi: 10.1016/j.fsi.2018.10.068. Epub 
      2018 Oct 26.