PMID- 30394391
OWN - NLM
STAT- MEDLINE
DCOM- 20190408
LR  - 20190408
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 140
DP  - 2018 Oct 18
TI  - Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a 
      Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates.
LID - 10.3791/58290 [doi]
LID - 58290
AB  - Spontaneously contracting syncytia of cardiomyocytes derived from human-induced 
      pluripotent stem cells (hiPSC-CM) are a useful model of human cardiac physiology 
      and pharmacology. Various methods have been proposed to record this spontaneous 
      activity and to evaluate drug effects, but many of these methods suffer from 
      limited throughput and/or physiological relevance. We developed a high-throughput 
      screening system to quantify the effects of exogenous compounds on hiPSC-CM's 
      beating frequency, using a Ca-sensitive fluorescent dye and a 
      temperature-controlled imaging multi-well plate reader. We describe how to 
      prepare the cell plates and the compound plates and how to run the automated 
      assay to achieve high sensitivity and reproducibility. We also describe how to 
      transform and analyze the fluorescence data to provide reliable measures of drug 
      effects on spontaneous rhythm. This assay can be used in drug discovery programs 
      to guide chemical optimization away from, or toward, compounds affecting human 
      cardiac function.
FAU - Forny, Camille
AU  - Forny C
AD  - Department of Pharmacology and Preclinical Development, Idorsia Pharmaceuticals 
      Ltd.
FAU - Sube, Romain
AU  - Sube R
AD  - Department of Pharmacology and Preclinical Development, Idorsia Pharmaceuticals 
      Ltd.
FAU - Ertel, Eric A
AU  - Ertel EA
AD  - Department of Pharmacology and Preclinical Development, Idorsia Pharmaceuticals 
      Ltd; eric.ertel@idorsia.com.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20181018
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Fluorescent Dyes/*pharmacology
MH  - Induced Pluripotent Stem Cells/*chemistry
MH  - Myocytes, Cardiac/*chemistry
MH  - Phenotype
MH  - Temperature
PMC - PMC6235566
EDAT- 2018/11/06 06:00
MHDA- 2019/04/09 06:00
PMCR- 2018/10/18
CRDT- 2018/11/06 06:00
PHST- 2018/11/06 06:00 [entrez]
PHST- 2018/11/06 06:00 [pubmed]
PHST- 2019/04/09 06:00 [medline]
PHST- 2018/10/18 00:00 [pmc-release]
AID - 58290 [pii]
AID - 10.3791/58290 [doi]
PST - epublish
SO  - J Vis Exp. 2018 Oct 18;(140):58290. doi: 10.3791/58290.