PMID- 30396921
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20181217
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 38
IP  - 11
DP  - 2018 Nov
TI  - Transcriptomic Analysis of Histone Methyltransferase Setd7 Knockdown and 
      Phenethyl Isothiocyanate in Human Prostate Cancer Cells.
PG  - 6069-6083
LID - 10.21873/anticanres.12957 [doi]
AB  - BACKGROUND/AIM: Transcriptomic analysis was performed to evaluate the 
      differential gene expression profiles of Setd7 knockdown (KD) and the effects of 
      phenethyl isothiocyanate (PEITC) in human prostate cancer (PCa) LNCaP cells. 
      MATERIALS AND METHODS: RNA isolated from wild-type and Setd7-KD LNCaP cells in 
      the presence or absence of PEITC was subjected to microarray analysis followed by 
      Ingenuity(R) Pathway Analysis (IPA). RESULTS: Setd7 KD impacted a larger set of 
      genes and caused a higher fold change compared to PEITC treatment. Several 
      signaling pathways were altered particularly inflammation-related TNFR signaling 
      and PTEN/PI3K/AKT signaling by Setd7 KD and PEITC. Interestingly, PEITC and Setd7 
      KD at a small subset of genes that could be potential molecular targets. 
      CONCLUSION: This study offers new insights into the mechanisms of action of the 
      epigenetic modifier Setd7 and the effects of PEITC treatment in PCa cells and 
      enhances our understanding of the potential cancer preventive/treatment effects 
      of isothiocyanate compounds such as PEITC in PCa.
CI  - Copyright(c) 2018, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Wang, Chao
AU  - Wang C
AD  - Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, the State 
      University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Center for Phytochemical Epigenome Studies, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
FAU - Sargsyan, Davit
AU  - Sargsyan D
AD  - Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, the State 
      University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Center for Phytochemical Epigenome Studies, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Graduate Program of Pharmaceutical Sciences, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
FAU - Zhang, Chengyue
AU  - Zhang C
AD  - Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, the State 
      University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Center for Phytochemical Epigenome Studies, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Graduate Program of Pharmaceutical Sciences, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
FAU - Wu, Renyi
AU  - Wu R
AD  - Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, the State 
      University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Center for Phytochemical Epigenome Studies, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
FAU - Yang, Yuqing
AU  - Yang Y
AD  - Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, the State 
      University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Center for Phytochemical Epigenome Studies, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
AD  - Graduate Program of Pharmaceutical Sciences, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
FAU - Kong, Ah-Ng
AU  - Kong AN
AD  - Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, the State 
      University of New Jersey, Piscataway, NJ, U.S.A. KongT@pharmacy.rutgers.edu.
AD  - Center for Phytochemical Epigenome Studies, Ernest Mario School of Pharmacy, 
      Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Isothiocyanates)
RN  - 6U7TFK75KV (phenethyl isothiocyanate)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (SETD7 protein, human)
SB  - IM
MH  - Cell Line, Tumor
MH  - Epigenesis, Genetic
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Gene Knockout Techniques
MH  - Histone-Lysine N-Methyltransferase/biosynthesis/deficiency/*genetics
MH  - Humans
MH  - Isothiocyanates/*pharmacology
MH  - Male
MH  - Prostatic Neoplasms/enzymology/genetics
MH  - Transcriptome
OTO - NOTNLM
OT  - Microarray
OT  - PEITC
OT  - Setd7
OT  - histone methyltransferase
OT  - isothiocyanates
OT  - prostate cancer
EDAT- 2018/11/07 06:00
MHDA- 2018/12/18 06:00
CRDT- 2018/11/07 06:00
PHST- 2018/07/25 00:00 [received]
PHST- 2018/09/19 00:00 [revised]
PHST- 2018/09/28 00:00 [accepted]
PHST- 2018/11/07 06:00 [entrez]
PHST- 2018/11/07 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
AID - 38/11/6069 [pii]
AID - 10.21873/anticanres.12957 [doi]
PST - ppublish
SO  - Anticancer Res. 2018 Nov;38(11):6069-6083. doi: 10.21873/anticanres.12957.