PMID- 30397194
OWN - NLM
STAT- MEDLINE
DCOM- 20190604
LR  - 20200309
IS  - 2092-6413 (Electronic)
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 50
IP  - 11
DP  - 2018 Nov 5
TI  - Adjuvant role of macrophages in stem cell-induced cardiac repair in rats.
PG  - 1-10
LID - 10.1038/s12276-018-0171-5 [doi]
LID - 143
AB  - Bone marrow-derived mesenchymal stem cells (BMMSCs) are used extensively for 
      cardiac repair and interact with immune cells in the damaged heart. Macrophages 
      are known to be modulated by stem cells, and we hypothesized that priming 
      macrophages with BMMSCs would enhance their therapeutic efficacy. Rat bone 
      marrow-derived macrophages (BMDMs) were stimulated by lipopolysaccharide (LPS) 
      with or without coculture with rat BMCs. In the LPS-stimulated BMDMs, induction 
      of the inflammatory marker iNOS was attenuated, and the anti-inflammatory marker 
      Arg1 was markedly upregulated by coculture with BMMSCs. Myocardial infarction 
      (MI) was induced in rats. One group was injected with BMMSCs, and a second group 
      was injected with MIX (a mixture of BMMSCs and BMDMs after coculture). The 
      reduction in cardiac fibrosis was greater in the MIX group than in the BMC group. 
      Cardiac function was improved in the BMMSC group and was substantially improved 
      in the MIX group. Angiogenesis was better in the MIX group, and anti-inflammatory 
      macrophages were more abundant in the MIX group than in the BMMSC group. In the 
      BMMSCs, interferon regulatory factor 5 (IRF5) was exclusively induced by 
      coculture with macrophages. IRF5 knockdown in BMMSCs failed to suppress 
      inflammatory marker induction in the macrophages. In this study, we demonstrated 
      the successful application of BMDMs primed with BMMSCs as an adjuvant to cell 
      therapy for cardiac repair.
FAU - Lim, Soo Yeon
AU  - Lim SY
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea.
FAU - Cho, Dong Im
AU  - Cho DI
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea.
FAU - Jeong, Hye-Yun
AU  - Jeong HY
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea.
FAU - Kang, Hye-Jin
AU  - Kang HJ
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea.
FAU - Kim, Mi Ra
AU  - Kim MR
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea.
FAU - Cho, Meeyoung
AU  - Cho M
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea.
FAU - Kim, Yong Sook
AU  - Kim YS
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea. retissue@jnu.ac.kr.
AD  - Biomedical Research Institute, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea. retissue@jnu.ac.kr.
FAU - Ahn, Youngkeun
AU  - Ahn Y
AD  - Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, 
      61469, Republic of Korea. cecilyk@hanmail.net.
AD  - Department of Cardiology, Chonnam National University Hospital, Gwangju, 61469, 
      Republic of Korea. cecilyk@hanmail.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181105
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Culture Media, Conditioned)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Animals
MH  - Arginase/genetics/metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/pharmacology
MH  - Humans
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/drug effects/metabolism
MH  - Mice
MH  - Myocardial Infarction/*therapy
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC6218450
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/11/07 06:00
MHDA- 2019/06/05 06:00
PMCR- 2018/11/05
CRDT- 2018/11/07 06:00
PHST- 2018/04/06 00:00 [received]
PHST- 2018/07/18 00:00 [accepted]
PHST- 2018/07/11 00:00 [revised]
PHST- 2018/11/07 06:00 [entrez]
PHST- 2018/11/07 06:00 [pubmed]
PHST- 2019/06/05 06:00 [medline]
PHST- 2018/11/05 00:00 [pmc-release]
AID - 10.1038/s12276-018-0171-5 [pii]
AID - 171 [pii]
AID - 10.1038/s12276-018-0171-5 [doi]
PST - epublish
SO  - Exp Mol Med. 2018 Nov 5;50(11):1-10. doi: 10.1038/s12276-018-0171-5.