PMID- 30397920
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20200930
IS  - 1365-2761 (Electronic)
IS  - 0140-7775 (Print)
IS  - 0140-7775 (Linking)
VI  - 42
IP  - 1
DP  - 2019 Jan
TI  - Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) 
      in Iceland caused by VHS virus genotype IV.
PG  - 47-62
LID - 10.1111/jfd.12910 [doi]
AB  - A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated 
      from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, 
      to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the 
      same facility, got infected. The virus isolated was identified as VHSV by ELISA 
      and real-time RT-PCR. Phylogenetic analysis, based on the glycoprotein (G) gene 
      sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled 
      laboratory exposure studies with this new isolate, there was 3% survival in the 
      I.P. injection challenged group while there was 90% survival in the immersion 
      group. VHSV was not re-isolated from fish challenged by immersion. In a 
      cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with 
      naive lumpfish as well as naive Atlantic salmon (Salmo salar L.). 10% of the 
      lumpfish shedders and 43%-50% of the cohabiting lumpfish survived after 4 weeks. 
      80%-92% of the Atlantic salmon survived, but no viral RNA was detected by 
      real-time RT-PCR nor VHSV was isolated from Atlantic salmon. This is the first 
      isolation of a notifiable virus in Iceland and the first report of VHSV of 
      genotype IV in European waters.
CI  - (c) 2018 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd.
FAU - Guethmundsdottir, Sigriethur
AU  - Guethmundsdottir S
AUID- ORCID: 0000-0003-4645-0229
AD  - Fish Disease Laboratory, Institute for Experimental Pathology, University of 
      Iceland, Reykjavik, Iceland.
FAU - Vendramin, Niccolo
AU  - Vendramin N
AUID- ORCID: 0000-0002-9217-7887
AD  - European Union Reference Laboratory for Fish Diseases, National Institute for 
      Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark.
FAU - Cuenca, Argelia
AU  - Cuenca A
AD  - European Union Reference Laboratory for Fish Diseases, National Institute for 
      Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark.
FAU - Sigurethardottir, Heietha
AU  - Sigurethardottir H
AD  - Fish Disease Laboratory, Institute for Experimental Pathology, University of 
      Iceland, Reykjavik, Iceland.
FAU - Kristmundsson, Arni
AU  - Kristmundsson A
AD  - Fish Disease Laboratory, Institute for Experimental Pathology, University of 
      Iceland, Reykjavik, Iceland.
FAU - Iburg, Tine Moesgaard
AU  - Iburg TM
AD  - European Union Reference Laboratory for Fish Diseases, National Institute for 
      Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark.
FAU - Olesen, Niels Jorgen
AU  - Olesen NJ
AD  - European Union Reference Laboratory for Fish Diseases, National Institute for 
      Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark.
LA  - eng
SI  - GENBANK/MH836523
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GR  - 634429/H2020 SFS-2014-2 ParaFishControl/
GR  - SI2.725290/European Union Reference Laboratory for Fish Diseases/
PT  - Journal Article
DEP - 20181105
PL  - England
TA  - J Fish Dis
JT  - Journal of fish diseases
JID - 9881188
RN  - 0 (Glycoproteins)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - Disease Outbreaks/veterinary
MH  - Fish Diseases/transmission/*virology
MH  - Genotype
MH  - Glycoproteins/genetics
MH  - Hemorrhagic Septicemia, Viral/genetics/*pathology/transmission
MH  - Iceland/epidemiology
MH  - Novirhabdovirus/classification/genetics/*pathogenicity
MH  - Perciformes/*virology
MH  - Phylogeny
MH  - RNA, Viral/isolation & purification
MH  - Salmo salar/virology
PMC - PMC7379627
OTO - NOTNLM
OT  - Iceland
OT  - challenge models
OT  - genotype IV
OT  - lumpfish
OT  - viral haemorrhagic septicaemia virus (VHSV)
EDAT- 2018/11/07 06:00
MHDA- 2019/01/15 06:00
PMCR- 2020/07/24
CRDT- 2018/11/07 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2018/08/31 00:00 [revised]
PHST- 2018/09/05 00:00 [accepted]
PHST- 2018/11/07 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2018/11/07 06:00 [entrez]
PHST- 2020/07/24 00:00 [pmc-release]
AID - JFD12910 [pii]
AID - 10.1111/jfd.12910 [doi]
PST - ppublish
SO  - J Fish Dis. 2019 Jan;42(1):47-62. doi: 10.1111/jfd.12910. Epub 2018 Nov 5.