PMID- 30398698
OWN - NLM
STAT- MEDLINE
DCOM- 20191007
LR  - 20191007
IS  - 1613-6829 (Electronic)
IS  - 1613-6810 (Linking)
VI  - 14
IP  - 52
DP  - 2018 Dec
TI  - Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for 
      Targeted Protein Delivery.
PG  - e1802618
LID - 10.1002/smll.201802618 [doi]
AB  - With a growing number of intracellular drug targets and the high efficacy of 
      protein therapeutics, the targeted delivery of active proteins with negligible 
      toxicity is a challenging issue in the field of precision medicine. Herein, a 
      programed assembly of nucleoprotein nanoparticles (NNPs) using DNA and zinc 
      fingers (ZnFs) for targeted protein delivery is presented. Two types of ZnFs with 
      different sequence specificities are genetically fused to a targeting moiety and 
      a protein cargo, respectively. Double-stranded DNA with multiple ZnF-binding 
      sequences is grafted onto inorganic nanoparticles, followed by conjugation with 
      the ZnF-fused proteins, generating the assembly of NNPs with a uniform size 
      distribution and high stability. The approach enables controlled loading of a 
      protein cargo on the NNPs, offering a high cytosolic delivery efficiency and 
      target specificity. The utility and potential of the assembly as a versatile 
      protein delivery vehicle is demonstrated based on their remarkable antitumor 
      activity and target specificity with negligible toxicity in a xenograft mice 
      model.
CI  - (c) 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Ryu, Yiseul
AU  - Ryu Y
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology (KAIST), Daejeon, 34141, South Korea.
FAU - Kang, Jung Ae
AU  - Kang JA
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute 
      (KAERI), Jeongup, 56212, South Korea.
FAU - Kim, Dasom
AU  - Kim D
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology (KAIST), Daejeon, 34141, South Korea.
FAU - Kim, Song-Rae
AU  - Kim SR
AD  - Division of Bio-Imaging, Korea Basic Science Institute (KBSI), Chuncheon, 24341, 
      South Korea.
FAU - Kim, Seungmin
AU  - Kim S
AD  - Department of Biochemistry, Kangwon National University, Chuncheon, 24341, South 
      Korea.
FAU - Park, Seong Ji
AU  - Park SJ
AD  - Department of Biochemistry, Kangwon National University, Chuncheon, 24341, South 
      Korea.
FAU - Kwon, Seung-Hae
AU  - Kwon SH
AD  - Division of Bio-Imaging, Korea Basic Science Institute (KBSI), Chuncheon, 24341, 
      South Korea.
FAU - Kim, Kil-Nam
AU  - Kim KN
AD  - Division of Bio-Imaging, Korea Basic Science Institute (KBSI), Chuncheon, 24341, 
      South Korea.
FAU - Lee, Dong-Eun
AU  - Lee DE
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute 
      (KAERI), Jeongup, 56212, South Korea.
FAU - Lee, Joong-Jae
AU  - Lee JJ
AUID- ORCID: 0000-0002-3121-1420
AD  - Department of Biochemistry, Kangwon National University, Chuncheon, 24341, South 
      Korea.
AD  - Institute of Life Sciences (ILS), Kangwon National University, Chuncheon, 24341, 
      South Korea.
FAU - Kim, Hak-Sung
AU  - Kim HS
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology (KAIST), Daejeon, 34141, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181106
PL  - Germany
TA  - Small
JT  - Small (Weinheim an der Bergstrasse, Germany)
JID - 101235338
RN  - 0 (Nucleoproteins)
RN  - 0 (Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - DNA/*chemistry
MH  - Drug Delivery Systems
MH  - Humans
MH  - Mice
MH  - Nanoparticles/*chemistry
MH  - Nucleoproteins/*chemistry
MH  - Protein Binding
MH  - Proteins/*chemistry
MH  - Zinc Fingers
OTO - NOTNLM
OT  - nanoparticles
OT  - nucleoproteins
OT  - protein delivery
OT  - self-assembly
OT  - targeted therapy
EDAT- 2018/11/07 06:00
MHDA- 2019/10/08 06:00
CRDT- 2018/11/07 06:00
PHST- 2018/07/05 00:00 [received]
PHST- 2018/09/29 00:00 [revised]
PHST- 2018/11/07 06:00 [pubmed]
PHST- 2019/10/08 06:00 [medline]
PHST- 2018/11/07 06:00 [entrez]
AID - 10.1002/smll.201802618 [doi]
PST - ppublish
SO  - Small. 2018 Dec;14(52):e1802618. doi: 10.1002/smll.201802618. Epub 2018 Nov 6.