PMID- 30399515 OWN - NLM STAT- MEDLINE DCOM- 20190415 LR - 20191210 IS - 1873-4235 (Electronic) IS - 0956-5663 (Linking) VI - 126 DP - 2019 Feb 1 TI - Reduced graphene oxide biosensor platform for the detection of NT-proBNP biomarker in its clinical range. PG - 136-142 LID - S0956-5663(18)30798-X [pii] LID - 10.1016/j.bios.2018.09.102 [doi] AB - Reduced graphene oxide (rGO) thin films can be exploited as highly sensitive transducer layers and integrated in interdigital micro-electrode systems for biosensing processes. The distinctive bipolar characterisitics of rGO thin films can be modulated by a very low external electric field due to the electrostatic charges of biomolecules. These charges lead to a fast response in the readout signals of rGO based ion sensitive field-effect transistors (ISFETs). The characterisitc changes of rGO ISFETs enable a fast, accurate and reproducible detection of biomolecules. The biosensing mechanism offers a fast and label-free approach for analyte detection in contrast to the classical ELISA method. In this contribution, we introduce a reproducible fabrication process of rGO based field-effect transistors on wafer level. The sensors are functionalized as biosensors to measure N-terminal pro-brain natriuretic peptide (NT-proBNP) in human serum within its clinical range. Our optimized rGO sensor shows very promising electrical properties and can be considered as a proof of concept study for the detection of various analytes. The easy and cost-effective fabrication as well as the versatile usability make this new technological platform an auspicious tool for different sensing applications in future. CI - Copyright (c) 2018 Elsevier B.V. All rights reserved. FAU - Munief, Walid-Madhat AU - Munief WM AD - Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, 66482 Zweibruecken, Germany; Department of Physical Chemistry, Saarland University, 66123 Saarbruecken, Germany; RAM Group DE GmbH, Research and Development Center, 66482 Zweibruecken, Germany. FAU - Lu, Xiaoling AU - Lu X AD - Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, 66482 Zweibruecken, Germany; Department of Electrical Engineering and Information Technology, IWE 1, RWTH Aachen University, 52074 Aachen, Germany. FAU - Teucke, Tobias AU - Teucke T AD - Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, 66482 Zweibruecken, Germany. FAU - Wilhelm, Jannick AU - Wilhelm J AD - RAM Group DE GmbH, Research and Development Center, 66482 Zweibruecken, Germany. FAU - Britz, Anette AU - Britz A AD - Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, 66482 Zweibruecken, Germany. FAU - Hempel, Felix AU - Hempel F AD - RAM Group DE GmbH, Research and Development Center, 66482 Zweibruecken, Germany. FAU - Lanche, Ruben AU - Lanche R AD - RAM Group DE GmbH, Research and Development Center, 66482 Zweibruecken, Germany. FAU - Schwartz, Miriam AU - Schwartz M AD - RAM Group DE GmbH, Research and Development Center, 66482 Zweibruecken, Germany. FAU - Law, Jessica Ka Yan AU - Law JKY AD - RAM Group DE GmbH, Research and Development Center, 66482 Zweibruecken, Germany. FAU - Grandthyll, Samuel AU - Grandthyll S AD - Department of Experimental Physics, Saarland University, Campus E2.9, 66123 Saarbruecken, Germany. FAU - Muller, Frank AU - Muller F AD - Department of Experimental Physics, Saarland University, Campus E2.9, 66123 Saarbruecken, Germany. FAU - Neurohr, Jens-Uwe AU - Neurohr JU AD - Department of Experimental Physics, Saarland University, Campus E2.9, 66123 Saarbruecken, Germany. FAU - Jacobs, Karin AU - Jacobs K AD - Department of Experimental Physics, Saarland University, Campus E2.9, 66123 Saarbruecken, Germany. FAU - Schmitt, Michael AU - Schmitt M AD - Institute for Coatings and Surface Technology, University of Applied Sciences Niederrhein, 47805 Krefeld, Germany. FAU - Pachauri, Vivek AU - Pachauri V AD - Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, 66482 Zweibruecken, Germany; Department of Electrical Engineering and Information Technology, IWE 1, RWTH Aachen University, 52074 Aachen, Germany. FAU - Hempelmann, Rolf AU - Hempelmann R AD - Department of Physical Chemistry, Saarland University, 66123 Saarbruecken, Germany; Korean Institute of Science and Technology, 66123 Saarbruecken, Germany. FAU - Ingebrandt, Sven AU - Ingebrandt S AD - Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, 66482 Zweibruecken, Germany; Department of Electrical Engineering and Information Technology, IWE 1, RWTH Aachen University, 52074 Aachen, Germany. LA - eng PT - Evaluation Study PT - Journal Article DEP - 20181017 PL - England TA - Biosens Bioelectron JT - Biosensors & bioelectronics JID - 9001289 RN - 0 (Biomarkers) RN - 0 (Peptide Fragments) RN - 0 (graphene oxide) RN - 0 (pro-brain natriuretic peptide (1-76)) RN - 114471-18-0 (Natriuretic Peptide, Brain) RN - 7782-42-5 (Graphite) SB - IM MH - Biomarkers/blood MH - Biosensing Techniques/*instrumentation MH - Electrodes MH - Equipment Design MH - Graphite/*chemistry MH - Humans MH - Limit of Detection MH - Natriuretic Peptide, Brain/*blood MH - Oxidation-Reduction MH - Peptide Fragments/*blood OTO - NOTNLM OT - Detection of NT-proBNP in the clinical range OT - New reduction concept OT - Reduced graphene oxide (rGO) thin-films OT - rGO ion sensitive field-effect transistor (ISFET) biosensor EDAT- 2018/11/07 06:00 MHDA- 2019/04/16 06:00 CRDT- 2018/11/07 06:00 PHST- 2018/07/12 00:00 [received] PHST- 2018/09/22 00:00 [revised] PHST- 2018/09/29 00:00 [accepted] PHST- 2018/11/07 06:00 [pubmed] PHST- 2019/04/16 06:00 [medline] PHST- 2018/11/07 06:00 [entrez] AID - S0956-5663(18)30798-X [pii] AID - 10.1016/j.bios.2018.09.102 [doi] PST - ppublish SO - Biosens Bioelectron. 2019 Feb 1;126:136-142. doi: 10.1016/j.bios.2018.09.102. Epub 2018 Oct 17.