PMID- 30400384 OWN - NLM STAT- MEDLINE DCOM- 20190131 LR - 20190131 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 19 IP - 11 DP - 2018 Nov 4 TI - Epidermal Fatty Acid-Binding Protein: A Novel Marker in the Diagnosis of Dry Eye Disease in Sjogren Syndrome. LID - 10.3390/ijms19113463 [doi] LID - 3463 AB - PURPOSE: Sjogren syndrome (SS) is a chronic inflammatory autoimmune disease of the lacrimal and salivary glands. This study compared the concentrations of epidermal fatty-acid binding protein (E-FABP) in the saliva, serum, and tears of SS patients with dry eye and dry mouth, with those of healthy adults to investigate the usefulness of E-FABP as a diagnostic marker for SS. DESIGN: Prospective, observational case series. PARTICIPANTS: The subjects were 11 new patients with untreated Sjogren syndrome and 12 healthy control individuals. METHODS: The diagnosis of SS was in accordance with the Ministry of Health, Labour and Welfare (Japan) Diagnostic Criteria (1999). Saliva, serum, and tear specimens were collected during internal medicine, dental, and ophthalmological examinations. The ophthalmological tests included the Dry Eye-related Quality of life Score (DEQS), tear break-up time (BUT), vital staining with fluorescein (FS) and lissamine green (LG), and the Schirmer test-1. The E-FABP concentration in the tears, saliva, and serum was measured by enzyme-linked immunosorbent assay (ELISA). MAIN OUTCOME MEASURE: The E-FABP concentrations were compared between patients and controls. RESULTS: There were significant differences between the patient and healthy control groups in all ophthalmological test results. There were no significant differences between the groups in the E-FABP concentrations in the saliva (p = 0.1513) or the serum (p = 0.4799), but the E-FABP concentration in the tears significantly differed between groups. The E-FABP concentration in tears tended to be significantly lower in patients with SS (mean, 323.5 +/- 325.6 pg/mL) than healthy control subjects (mean, 4076 pg/mL; p = 0.0136). The E-FABP concentration in tears significantly correlated with the results of dry eye parameters. CONCLUSION: The E-FABP concentration in tears appears to be related to ocular surface epithelial damage and tear stability and may be a promising novel biomarker in the diagnosis of SS. FAU - Shinzawa, Megumi AU - Shinzawa M AD - Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. mshinz2003@yahoo.co.jp. AD - Department of Ophthalmology, Keio University School of Medicine, Tokyo 1608582, Japan. mshinz2003@yahoo.co.jp. FAU - Dogru, Murat AU - Dogru M AD - Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. muratodooru2005@yahoo.co.jp. AD - Department of Ophthalmology, Keio University School of Medicine, Tokyo 1608582, Japan. muratodooru2005@yahoo.co.jp. FAU - Den, Seika AU - Den S AD - Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. denseika@gmail.com. FAU - Ichijima, Takehiro AU - Ichijima T AD - Department of Dental and Oral Surgery, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. ichijimatakehiro@gmail.com. FAU - Higa, Kazunari AU - Higa K AD - Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. higakazunari@tdc.ac.jp. FAU - Kojima, Takashi AU - Kojima T AD - Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. kojkoj@me.com. AD - Department of Ophthalmology, Keio University School of Medicine, Tokyo 1608582, Japan. kojkoj@me.com. FAU - Seta, Noriyuki AU - Seta N AD - Department of Internal Medicine, Tokyo Dental College, Ichikawa General Hospital, 2728513 Chiba, Japan. nseta@tdc.ac.jp. FAU - Nomura, Takeshi AU - Nomura T AD - Department of Dental and Oral Surgery, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. tanomura@tdc.ac.jp. FAU - Tsubota, Kazuo AU - Tsubota K AD - Department of Ophthalmology, Keio University School of Medicine, Tokyo 1608582, Japan. tsubota@eyebank.or.jp. FAU - Shimazaki, Jun AU - Shimazaki J AD - Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba 2728513, Japan. jun@eyebank.or.jp. LA - eng GR - 2016/Tokyo Dental College University President Grant/ PT - Journal Article PT - Observational Study DEP - 20181104 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers) RN - 0 (FABP5 protein, human) RN - 0 (Fatty Acid-Binding Proteins) SB - IM MH - Adult MH - Aged MH - Biomarkers/metabolism MH - Case-Control Studies MH - Fatty Acid-Binding Proteins/*genetics/metabolism MH - Female MH - Gene Expression MH - Humans MH - Male MH - Middle Aged MH - Prospective Studies MH - Quality of Life/psychology MH - Saliva/chemistry MH - Sjogren's Syndrome/*diagnosis/genetics/metabolism/psychology MH - Tears/chemistry MH - Xerophthalmia/*diagnosis/genetics/metabolism/psychology PMC - PMC6274910 OTO - NOTNLM OT - dry eye OT - epidermal fatty-acid binding protein (E-FABP), Sjogren's syndrome OT - tears COIS- The authors declare no conflicts of interest. EDAT- 2018/11/08 06:00 MHDA- 2019/02/01 06:00 PMCR- 2018/11/01 CRDT- 2018/11/08 06:00 PHST- 2018/09/22 00:00 [received] PHST- 2018/10/23 00:00 [revised] PHST- 2018/10/27 00:00 [accepted] PHST- 2018/11/08 06:00 [entrez] PHST- 2018/11/08 06:00 [pubmed] PHST- 2019/02/01 06:00 [medline] PHST- 2018/11/01 00:00 [pmc-release] AID - ijms19113463 [pii] AID - ijms-19-03463 [pii] AID - 10.3390/ijms19113463 [doi] PST - epublish SO - Int J Mol Sci. 2018 Nov 4;19(11):3463. doi: 10.3390/ijms19113463.