PMID- 30402831
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20191217
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 20
DP  - 2018 Oct
TI  - Up-regulation of miR-517-5p inhibits ERK/MMP-2 pathway: potential role in 
      preeclampsia.
PG  - 6599-6608
LID - 16134 [pii]
LID - 10.26355/eurrev_201810_16134 [doi]
AB  - OBJECTIVE: To investigate the potential role of miRNA-517-5p in preeclampsia and 
      its underlying mechanism. MATERIALS AND METHODS: Placenta samples were obtained 
      from 20 women with preeclampsia and 20 women with normal pregnancies. Expression 
      level of miR-517-5p in placenta samples and JAR cells was detected. MiRNA-517-5p 
      mimics or inhibitor was transfected in JAR cells, followed by detection of 
      proliferative and invasive abilities of JAR cells. In addition, the expressions 
      of extracellular regulated protein kinases (ERK), phospho-extracellular regulated 
      protein kinases (p-ERK) and matrix metalloproteinase-2 (MMP-2) in JAR cells were 
      evaluated by Western blot. Meanwhile, the mRNA level of MMP-2 was evaluated by 
      Real-time polymerase chain reaction (PCR). The luciferase assay was applied to 
      identify the target gene of miRNA-517-5p. RESULTS: Increased level of miR-517-5p 
      was detected in placenta samples of preeclampsia patients compared with normal 
      pregnancies. MiRNA-517-5p could regulate proliferative and invasive abilities of 
      JAR cells. Furthermore, miRNA-517-5p could regulate ERK/MMP-2 pathway in JAR 
      cells, which would contribute to the pathophysiology of preeclampsia. The 
      luciferase assay showed MMP-2 was the target gene of miR-517-5p. Further studies 
      showed that MMP-2 was dysregulated in preeclampsia. CONCLUSIONS: MiR-517-5p is 
      highly expressed in placenta samples of preeclampsia pregnancies, which could 
      promote proliferative and invasive abilities of JAR cells by inhibiting ERK/MMP-2 
      pathway.
FAU - Fu, J-Y
AU  - Fu JY
AD  - Affiliated Hospital of Chengde Medical College, Chengde, China. 
      wenbi5276@163.com.
FAU - Xiao, Y-P
AU  - Xiao YP
FAU - Ren, C-L
AU  - Ren CL
FAU - Guo, Y-W
AU  - Guo YW
FAU - Qu, D-H
AU  - Qu DH
FAU - Zhang, J-H
AU  - Zhang JH
FAU - Zhu, Y-J
AU  - Zhu YJ
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN517 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Blood Pressure
MH  - Case-Control Studies
MH  - Cell Line
MH  - Cell Proliferation
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 2/genetics/*metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Placenta/*enzymology/pathology/physiopathology
MH  - Pre-Eclampsia/*enzymology/genetics/pathology/physiopathology
MH  - Pregnancy
MH  - Signal Transduction
MH  - Up-Regulation
EDAT- 2018/11/08 06:00
MHDA- 2019/12/18 06:00
CRDT- 2018/11/08 06:00
PHST- 2018/11/08 06:00 [entrez]
PHST- 2018/11/08 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
AID - 16134 [pii]
AID - 10.26355/eurrev_201810_16134 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 Oct;22(20):6599-6608. doi: 
      10.26355/eurrev_201810_16134.