PMID- 30405071
OWN - NLM
STAT- MEDLINE
DCOM- 20190211
LR  - 20211204
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 19
IP  - 11
DP  - 2018 Nov 7
TI  - Study on Cardiotoxicity and Mechanism of "Fuzi" Extracts Based on Metabonomics.
LID - 10.3390/ijms19113506 [doi]
LID - 3506
AB  - To investigate the toxicity of water and ethanol "Fuzi" (FZ) extracts and to 
      explore the toxicity mechanism in rats. Water and ethanol extracts were prepared. 
      Three groups of rats received the water extract, ethanol extract, or water by 
      oral gavage for seven days. Pathological section staining of heart tissue. 
      Colorimetric analysis was used to determine serum lactate dehydrogenase. The 
      metabolic expression of small molecules in rats was measured by a metabolomics 
      method. Western blotting was used to detect the expression of phosphoinositide 
      3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), 
      transforming growth factor-beta1 (TGF-beta1), and caspase-3. Immunohistochemistry was 
      used to detect the expression of CTnI, mTOR, and TGF-beta1. The water and ethanol FZ 
      extracts exert cardiotoxic effects via activating the PI3K/Akt/mTOR signaling 
      pathway to induce cardiomyocyte apoptosis.
FAU - Huang, Guangyao
AU  - Huang G
AD  - Department of Pharmacology, Basic Medical College, Anhui Medical University, 
      Hefei 230032, China. huangguangyao@stu.ahmu.edu.cn.
AD  - Department of Pharmacology and Toxicology, Beijing Institute of Radiation 
      Medicine, Beijing 100850, China. huangguangyao@stu.ahmu.edu.cn.
FAU - Yang, Liang
AU  - Yang L
AD  - Department of Pharmacology and Toxicology, Beijing Institute of Radiation 
      Medicine, Beijing 100850, China. jkliang_yang@126.com.
FAU - Zhou, Wei
AU  - Zhou W
AUID- ORCID: 0000-0003-0435-7261
AD  - Department of Pharmacology and Toxicology, Beijing Institute of Radiation 
      Medicine, Beijing 100850, China. zhouweisyl802@163.com.
FAU - Tang, Xianglin
AU  - Tang X
AD  - Department of Pharmacology and Toxicology, Beijing Institute of Radiation 
      Medicine, Beijing 100850, China. tangxianglin@139.com.
FAU - Wang, Yuguang
AU  - Wang Y
AD  - Department of Pharmacology and Toxicology, Beijing Institute of Radiation 
      Medicine, Beijing 100850, China. wyg79@163.com.
FAU - Ma, Zengchun
AU  - Ma Z
AD  - Department of Pharmacology and Toxicology, Beijing Institute of Radiation 
      Medicine, Beijing 100850, China. mazchun@139.com.
FAU - Gao, Shan
AU  - Gao S
AD  - Department of Pharmacology, Basic Medical College, Anhui Medical University, 
      Hefei 230032, China. gaoshan@ahmu.edu.cn.
FAU - Gao, Yue
AU  - Gao Y
AD  - Department of Pharmacology and Toxicology, Beijing Institute of Radiation 
      Medicine, Beijing 100850, China. gaoyue@nic.bmi.ac.cn.
LA  - eng
GR  - 81630102/National Natural Science Foundation of China/
GR  - 81673633/National Natural Science Foundation of China/
GR  - 81703669/National Natural Science Foundation of China/
GR  - 2017ZX09301012-007/National Science and Technology Major Project of China/
PT  - Journal Article
DEP - 20181107
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Diterpenes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Plant Extracts)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Troponin I)
RN  - 0 (fuzi drug herbal)
RN  - 059QF0KO0R (Water)
RN  - 3K9958V90M (Ethanol)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cardiotoxicity/blood/*metabolism/pathology
MH  - Caspase 3/metabolism
MH  - Diterpenes
MH  - Drugs, Chinese Herbal
MH  - Ethanol
MH  - Male
MH  - Metabolome
MH  - *Metabolomics
MH  - Myocardium/metabolism/pathology
MH  - Myocytes, Cardiac/metabolism/pathology
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Plant Extracts/*toxicity
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Transforming Growth Factor beta1/metabolism
MH  - Troponin I/metabolism
MH  - Water
PMC - PMC6274692
OTO - NOTNLM
OT  - Fuzi
OT  - PI3K/Akt/mTOR
OT  - cardiotoxicity
OT  - caspase-3
OT  - metabolomics
COIS- The authors declare that they have no competing interests to disclose.
EDAT- 2018/11/09 06:00
MHDA- 2019/02/12 06:00
PMCR- 2018/11/01
CRDT- 2018/11/09 06:00
PHST- 2018/09/19 00:00 [received]
PHST- 2018/10/26 00:00 [revised]
PHST- 2018/11/05 00:00 [accepted]
PHST- 2018/11/09 06:00 [entrez]
PHST- 2018/11/09 06:00 [pubmed]
PHST- 2019/02/12 06:00 [medline]
PHST- 2018/11/01 00:00 [pmc-release]
AID - ijms19113506 [pii]
AID - ijms-19-03506 [pii]
AID - 10.3390/ijms19113506 [doi]
PST - epublish
SO  - Int J Mol Sci. 2018 Nov 7;19(11):3506. doi: 10.3390/ijms19113506.