PMID- 30406042
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20190909
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 8
DP  - 2018
TI  - Identification of Anti-staphylococcal and Anti-biofilm Compounds by Repurposing 
      the Medicines for Malaria Venture Pathogen Box.
PG  - 365
LID - 10.3389/fcimb.2018.00365 [doi]
LID - 365
AB  - There has been an alarming increase in infections caused by 
      antimicrobial-resistant pathogens. These infections are responsible for more than 
      half a million deaths globally each year. Staphylococcus aureus is one of the 
      deadliest bacterial pathogen responsible for nosocomial and community acquired 
      infections. The open-access Pathogen Box (PBox) provides a potential platform to 
      identify new treatment options against antibiotic-resistant bacteria by 
      repurposing it. In this study, we have screened the PBox library comprised of 
      ~400 compounds to identify novel anti-staphylococcal compounds. in vitro 
      antimicrobial screening using S. aureus isolates, ATCC 29213 
      (methicillin-sensitive) and ATCC 700699 (methicillin-resistant) revealed 13 
      compounds which showed highly potent antibacterial activity against both 
      planktonic and biofilm state. The 13 compounds were not found cytotoxic to mouse 
      macrophage cell line, RAW264.7. Out of the 13 compounds, only MMV687251 and 
      MMV676477 revealed structural similarity with vancomycin by comparing their 
      atomic pair fingerprints using Tanimoto coefficient method. The structural 
      similarities may indicate similar mode of action like vancomycin for the two 
      compounds. Our result showed that PBox compounds offer a promising lead for the 
      development of new anti-staphylococcal treatment options.
FAU - Bhandari, Vasundhra
AU  - Bhandari V
AD  - National Institute of Animal Biotechnology, Hyderabad, India.
FAU - Chakraborty, Shalini
AU  - Chakraborty S
AD  - National Institute of Animal Biotechnology, Hyderabad, India.
FAU - Brahma, Umarani
AU  - Brahma U
AD  - National Institute of Animal Biotechnology, Hyderabad, India.
FAU - Sharma, Paresh
AU  - Sharma P
AD  - National Institute of Animal Biotechnology, Hyderabad, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181023
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/*isolation & purification/*pharmacology/toxicity
MH  - Biofilms/*drug effects
MH  - Cell Survival/drug effects
MH  - Drug Evaluation, Preclinical
MH  - *Drug Repositioning
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - RAW 264.7 Cells/drug effects
MH  - Staphylococcal Infections/*microbiology
MH  - Staphylococcus aureus/*drug effects
PMC - PMC6206229
OTO - NOTNLM
OT  - anti-biofilm
OT  - anti-staphylococcal
OT  - antimicrobial resistance
OT  - open source drug discovery
OT  - repurposing
EDAT- 2018/11/09 06:00
MHDA- 2019/09/10 06:00
PMCR- 2018/01/01
CRDT- 2018/11/09 06:00
PHST- 2018/05/11 00:00 [received]
PHST- 2018/10/01 00:00 [accepted]
PHST- 2018/11/09 06:00 [entrez]
PHST- 2018/11/09 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2018.00365 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2018 Oct 23;8:365. doi: 10.3389/fcimb.2018.00365. 
      eCollection 2018.