PMID- 30406874
OWN - NLM
STAT- MEDLINE
DCOM- 20190610
LR  - 20200225
IS  - 1532-2807 (Electronic)
IS  - 1219-4956 (Linking)
VI  - 25
IP  - 2
DP  - 2019 Apr
TI  - Triiodothyronine Promotes Cell Proliferation of Breast Cancer via Modulating 
      miR-204/Amphiregulin.
PG  - 653-658
LID - 10.1007/s12253-018-0525-2 [doi]
AB  - Breast cancer (BC) severely threatens women's life, and Triiodothyronine (T3) 
      shows a positive role on BC cell proliferation, while the potential mechanism 
      underlying it is still unclear. T3 was used to stimulate BC cell lines MCF-7 and 
      T47-D. Real-time PCR was performed to determine the expression of miRNAs, while 
      western blot was used to measure protein expression of Amphiregulin (AREG), AKT 
      and p-AKT. The interaction between miR-204 and AREG was determined using 
      luciferase reporter assay. MTT was performed to detect cell viability. The 
      expression of miR-204 was decreased, while AREG and p-AKT was increased in T3 
      stimulated BC cell lines. T3 stimulation promoted cell viability. miR-204 targets 
      AREG to regulate its expression. T3 promoted expression of AREG and p-AKT, while 
      miR-204 overexpression reversed the effect of T3, however, pcDNA-AREG 
      transfection abolished the effect of miR-204 mimic. T3 promoted cell viability of 
      BC cells via modulating the AKT signaling pathway. The detailed mechanism was 
      that the down-regulated miR-204 that induced by T3 stimulation promoted the 
      expression of AREG, the up-regulated AREG activated AKT signaling pathway, while 
      the activated AKT signaling promoted cell proliferation.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Breast and Thyroid Surgery, Tongliao City Hospital, No.668 Keerqin 
      Rd., Keerqin District, Tongliao, 028000, Inner Mongolia, China. wupi5885@163.com.
FAU - Zhang, Fengxiang
AU  - Zhang F
AD  - Department of Breast and Thyroid Surgery, Tongliao City Hospital, No.668 Keerqin 
      Rd., Keerqin District, Tongliao, 028000, Inner Mongolia, China.
FAU - Li, Yanxin
AU  - Li Y
AD  - Department of Breast and Thyroid Surgery, Tongliao City Hospital, No.668 Keerqin 
      Rd., Keerqin District, Tongliao, 028000, Inner Mongolia, China.
FAU - Qi, Xiangjun
AU  - Qi X
AD  - Department of Breast and Thyroid Surgery, Tongliao City Hospital, No.668 Keerqin 
      Rd., Keerqin District, Tongliao, 028000, Inner Mongolia, China.
FAU - Guo, Yaming
AU  - Guo Y
AD  - Department of Breast and Thyroid Surgery, Tongliao City Hospital, No.668 Keerqin 
      Rd., Keerqin District, Tongliao, 028000, Inner Mongolia, China.
LA  - eng
PT  - Journal Article
DEP - 20181108
PL  - Switzerland
TA  - Pathol Oncol Res
JT  - Pathology oncology research : POR
JID - 9706087
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (MIRN204 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 06LU7C9H1V (Triiodothyronine)
SB  - IM
MH  - Amphiregulin/*biosynthesis
MH  - Breast Neoplasms/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/drug effects/*physiology
MH  - Humans
MH  - MicroRNAs/*biosynthesis
MH  - Signal Transduction/drug effects/physiology
MH  - Triiodothyronine/*pharmacology
OTO - NOTNLM
OT  - AREG
OT  - Breast cancer
OT  - Cell viability
OT  - T3
OT  - miR-204
EDAT- 2018/11/09 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/11/09 06:00
PHST- 2018/09/20 00:00 [received]
PHST- 2018/10/25 00:00 [accepted]
PHST- 2018/11/09 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/11/09 06:00 [entrez]
AID - 10.1007/s12253-018-0525-2 [pii]
AID - 10.1007/s12253-018-0525-2 [doi]
PST - ppublish
SO  - Pathol Oncol Res. 2019 Apr;25(2):653-658. doi: 10.1007/s12253-018-0525-2. Epub 
      2018 Nov 8.