PMID- 3040702
OWN - NLM
STAT- MEDLINE
DCOM- 19870930
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 262
IP  - 24
DP  - 1987 Aug 25
TI  - Calcium-independent phosphoinositide breakdown in rat basophilic leukemia cells. 
      Evidence for an early rise in inositol 1,4,5-trisphosphate which precedes the 
      rise in other inositol phosphates and in cytoplasmic calcium.
PG  - 11449-54
AB  - Aggregation of the receptor with high affinity for immunoglobulin E (IgE) in rat 
      basophilic leukemia cells leads to a calcium-dependent and a calcium-independent 
      hydrolysis of phosphoinositides. The increase in the levels of inositol 
      phosphates induced in the absence of calcium is only 25% of that observed with 1 
      mM Ca2+. The inositol phosphates reach a new steady state level 2 min after 
      stimulation in EGTA, whereas with calcium they continue to increase up to 15 min. 
      A similar response is observed when the receptors are aggregated due to the 
      interaction of bound IgE with antigen or with anit-IgE, or by the binding of IgE 
      cross-linked chemically. The antigen-mediated response is inhibited by hapten and 
      disruption of such antigen-antibody aggregates late after stimulation leads to a 
      rapid decline in the levels of the inositol phosphates to basal values. 
      Separation of the inositol phosphates by Dowex columns shows that there is a fast 
      rise in inositol trisphosphate which peaks at 15 s and slowly declines to a lower 
      plateau within 2 min. Analysis by high pressure liquid chromatography reveals a 
      5-fold increase in the levels of inositol 1,4,5-trisphosphate in less than 10 s 
      after stimulation, which precedes any major change in the other inositol 
      phosphates. Aggregation of the receptor in the absence of external calcium 
      induces a transient increase in cytoplasmic calcium which reaches a maximum of 
      approximately 25 nM over basal levels after activation. The onset of the rise in 
      Ca2+ lags after the initial rise in the inositol 1,4,5-trisphosphate.
FAU - Pribluda, V S
AU  - Pribluda VS
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Dinitrophenols)
RN  - 0 (Inositol Phosphates)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Sugar Phosphates)
RN  - 0 (dinitrophenyl-bovine serum albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Basophils/*metabolism
MH  - Calcium/*blood
MH  - Cell Line
MH  - Chromatography, High Pressure Liquid
MH  - Dinitrophenols/metabolism
MH  - Inositol 1,4,5-Trisphosphate
MH  - Inositol Phosphates/*blood
MH  - Leukemia, Experimental/*blood
MH  - Phosphatidylinositols/*blood
MH  - Rats
MH  - Receptors, Fc/metabolism
MH  - Receptors, IgE
MH  - Receptors, Immunologic/metabolism
MH  - Serum Albumin, Bovine/metabolism
MH  - Sugar Phosphates/*blood
EDAT- 1987/08/25 00:00
MHDA- 1987/08/25 00:01
CRDT- 1987/08/25 00:00
PHST- 1987/08/25 00:00 [pubmed]
PHST- 1987/08/25 00:01 [medline]
PHST- 1987/08/25 00:00 [entrez]
AID - S0021-9258(18)60827-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1987 Aug 25;262(24):11449-54.