PMID- 30407411
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2072-666X (Print)
IS  - 2072-666X (Electronic)
IS  - 2072-666X (Linking)
VI  - 7
IP  - 3
DP  - 2016 Feb 27
TI  - Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc.
LID - 10.3390/mi7030038 [doi]
LID - 38
AB  - A lab-on-a-disc (LOAD) is a centrifugal microfluidic set-up based on centrifugal 
      force without using micro-pumps to drive reagents and cells to various chambers 
      through channels and valves for reactions. A LOAD coated with conductive 
      transparent indium tin oxide (ITO) for thermal control was developed to screen 
      allergy-blocking agents. When the acridine orange (AO)-loaded KU-812 human 
      basophilic cells were activated in the LOAD by stimuli, AO trapped in the 
      cytoplasmic granules was released externally as an allergic mediator mimetic to 
      report degranulation. This response was monitored by fluorescence when the 
      released AO in supernatant had been transferred, with a higher spinning speed, 
      from the reaction chamber to detection chamber in the LOAD where AO reacted with 
      exogenous DNA. We report here the principles of the system and an improved LOAD 
      set-up with the ITO-coated glass resistive microheater to run assays at 37  degrees C. By 
      using this platform, we demonstrate here for the first time that triptolide, an 
      active ingredient from the Chinese medicine herb Tripterygium wilfordii Hook f., 
      was able to suppress the fMLP-mediated degranulation in basophils. This serves as 
      an example how LOADs can be used to screen agents to alleviate symptoms of 
      allergy.
FAU - Kwok, Ho Chin
AU  - Kwok HC
AD  - Department of Electronic Engineering, Center for Advanced Research in Photonics, 
      The Chinese University of Hong Kong, Shatin, Hong Kong, China. 
      hckwok@ee.cuhk.edu.hk.
FAU - Lau, Pui Man
AU  - Lau PM
AD  - Programme of Biochemistry, School of Life Sciences, The Chinese University of 
      Hong Kong, Shatin, Hong Kong, China. irenelau@cuhk.edu.hk.
FAU - Wu, Shu Yuen
AU  - Wu SY
AD  - Department of Electronic Engineering, Center for Advanced Research in Photonics, 
      The Chinese University of Hong Kong, Shatin, Hong Kong, China. 
      sywu@ee.cuhk.edu.hk.
FAU - Ho, Ho Pui
AU  - Ho HP
AD  - Department of Electronic Engineering, Center for Advanced Research in Photonics, 
      The Chinese University of Hong Kong, Shatin, Hong Kong, China. 
      hpho@ee.cuhk.edu.hk.
FAU - Gao, Minghui
AU  - Gao M
AD  - Programme of Biochemistry, School of Life Sciences, The Chinese University of 
      Hong Kong, Shatin, Hong Kong, China. minghuigao0511@gmail.com.
FAU - Kwan, Yiu Wa
AU  - Kwan YW
AD  - School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong 
      Kong, China. yiuwakwan@cuhk.edu.hk.
FAU - Wong, Chun Kwok
AU  - Wong CK
AD  - Department of Chemical Pathology, Prince of Wales Hospital, The Chinese 
      University of Hong Kong, Shatin, Hong Kong, China. ck-wong@cuhk.edu.hk.
AD  - Institute of Chinese Medicine and State Key Laboratory of Phytochemistry and 
      Plant Resources in West China, The Chinese University of Hong Kong, Shatin, Hong 
      Kong, China. ck-wong@cuhk.edu.hk.
FAU - Kong, Siu Kai
AU  - Kong SK
AD  - Programme of Biochemistry, School of Life Sciences, The Chinese University of 
      Hong Kong, Shatin, Hong Kong, China. skkong@cuhk.edu.hk.
LA  - eng
GR  - 466012/GRF grant, Hong Kong Special Administrative Region/
GR  - 412613/GRF grant, Hong Kong Special Administrative Region/
GR  - CUHK1/CRF/12G/CRF grant, Hong Kong Special Administrative Region/
GR  - 4930722/CUHK VC Discretionary Fund/
GR  - PF09-06613/Hong Kong PhD Fellowship/
PT  - Journal Article
DEP - 20160227
PL  - Switzerland
TA  - Micromachines (Basel)
JT  - Micromachines
JID - 101640903
PMC - PMC6189934
OTO - NOTNLM
OT  - N-formyl-methionine-leucine-phenylalanine (fMLP)
OT  - Traditional Chinese Medicine (TCM)
OT  - World Allergy Organization (WAO)
OT  - acridine orange (AO)
OT  - basophil activation test (BAT)
OT  - bisphenol A (BPA)
OT  - indium tin oxide (ITO)
OT  - lab-on-a-disc (LOAD)
OT  - phorbol 12-myristate 13-acetate (PMA)
OT  - protein kinase C (PKC)
COIS- All authors declare no conflict of interest.
EDAT- 2016/02/27 00:00
MHDA- 2016/02/27 00:01
PMCR- 2016/02/27
CRDT- 2018/11/09 06:00
PHST- 2015/12/07 00:00 [received]
PHST- 2016/02/15 00:00 [revised]
PHST- 2016/02/24 00:00 [accepted]
PHST- 2018/11/09 06:00 [entrez]
PHST- 2016/02/27 00:00 [pubmed]
PHST- 2016/02/27 00:01 [medline]
PHST- 2016/02/27 00:00 [pmc-release]
AID - mi7030038 [pii]
AID - micromachines-07-00038 [pii]
AID - 10.3390/mi7030038 [doi]
PST - epublish
SO  - Micromachines (Basel). 2016 Feb 27;7(3):38. doi: 10.3390/mi7030038.