PMID- 30407703
OWN - NLM
STAT- MEDLINE
DCOM- 20200122
LR  - 20200122
IS  - 1365-2982 (Electronic)
IS  - 1350-1925 (Linking)
VI  - 31
IP  - 3
DP  - 2019 Mar
TI  - Upregulation of intestinal mucosal mast cells expressing VPAC1 in close proximity 
      to vasoactive intestinal polypeptide in inflammatory bowel disease and murine 
      colitis.
PG  - e13503
LID - 10.1111/nmo.13503 [doi]
AB  - BACKGROUND: Mast cells (MCs) and vasoactive intestinal polypeptide (VIP) have 
      been proposed as regulators of the intestinal barrier and inflammation. Our aim 
      was to map the distribution in inflammatory bowel disease (IBD) and murine 
      colitis. METHODS: MCs, VIP, and VIP-receptors (VPACs) were quantified by 
      immunofluorescence and enzyme-immunoassay (EIA) in ileal tissues (villus 
      epithelium (VE) and adjacent VE, ie, VE next to the follicle-associated 
      epithelium, (FAE)) from Crohn's disease (CD; n = 16) and non-IBD patients, and in 
      colonic specimens of ulcerative colitis (UC; n = 12) and healthy controls (HCs). 
      In addition, VIP levels were measured in plasma from HCs, non-IBD, and IBD in 
      remission (CD n = 30; UC n = 30). Colon, ileum, and plasma from mice with dextran 
      sulfate sodium (DSS)-induced colitis and control mice were analyzed likewise. KEY 
      RESULTS: FAE-adjacent VE in ileum of CD possessed more MCs (P < 0.05) and MCs 
      expressing VPAC1 (P < 0.05), but not VPAC2, compared to controls. Both adjacent 
      and regular VE of CD had more MCs co-localizing/in close proximity to VIP 
      (P < 0.05). In UC colon, more MCs (P < 0.0005), MCs close to VIP (P < 0.0005), 
      and MCs expressing VPAC1 (P < 0.05) were found compared to controls. VIP levels 
      were elevated in plasma from CD and UC compared to controls (P < 0.0005). Colon 
      of DSS mice showed more MCs and MCs close to VIP (P < 0.05) compared to control 
      mice. In vitro experiments revealed MCs expressing VPACs and internalized VIP 
      after 120 minutes of VIP-stimulation. CONCLUSIONS AND INFERENCES: Communication 
      between MCs and VIP is upregulated during IBD and mice colitis. In CD patients, 
      the epithelium next to FAE seems to be more involved than the surrounding VE, 
      suggesting increased MC-VIP-interactions in this intestinal region.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Casado-Bedmar, Maite
AU  - Casado-Bedmar M
AD  - Department of Clinical and Experimental Medicine, Division of Surgery, 
      Orthopedics & Oncology, Linkoping University, Linkoping, Sweden.
FAU - Heil, Stephanie D S
AU  - Heil SDS
AD  - Department of Clinical and Experimental Medicine, Division of Surgery, 
      Orthopedics & Oncology, Linkoping University, Linkoping, Sweden.
FAU - Myrelid, Par
AU  - Myrelid P
AD  - Department of Clinical and Experimental Medicine, Division of Surgery, 
      Orthopedics & Oncology, Linkoping University, Linkoping, Sweden.
AD  - Department of Surgery, County Council of Ostergotland, Linkoping, Sweden.
FAU - Soderholm, Johan D
AU  - Soderholm JD
AD  - Department of Clinical and Experimental Medicine, Division of Surgery, 
      Orthopedics & Oncology, Linkoping University, Linkoping, Sweden.
AD  - Department of Surgery, County Council of Ostergotland, Linkoping, Sweden.
FAU - Keita, Asa V
AU  - Keita AV
AUID- ORCID: 0000-0002-6820-0215
AD  - Department of Clinical and Experimental Medicine, Division of Surgery, 
      Orthopedics & Oncology, Linkoping University, Linkoping, Sweden.
LA  - eng
GR  - 2014-02537/Swedish Research Council/International
GR  - 2017-02475/Swedish Research Council/International
GR  - RB13-016/Swedish Foundation for Strategic Research/International
GR  - Lions Clubs International Foundation/International
GR  - Mattssons Fastighetsutveckling i Stockholm AB/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181108
PL  - England
TA  - Neurogastroenterol Motil
JT  - Neurogastroenterology and motility
JID - 9432572
RN  - 0 (Receptors, Vasoactive Intestinal Polypeptide, Type I)
RN  - 0 (VIPR1 protein, human)
RN  - 0 (Vipr1 protein, mouse)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Cell Count
MH  - Colitis, Ulcerative/chemically induced/*metabolism
MH  - Crohn Disease/metabolism
MH  - Dextran Sulfate
MH  - Female
MH  - Humans
MH  - Ileum/metabolism
MH  - Inflammatory Bowel Diseases/*metabolism/*pathology
MH  - Intestinal Mucosa/*metabolism/pathology
MH  - Male
MH  - Mast Cells/*metabolism/*pathology
MH  - Mice, Inbred BALB C
MH  - Middle Aged
MH  - Receptors, Vasoactive Intestinal Polypeptide, Type I/*biosynthesis/genetics
MH  - Up-Regulation
MH  - Vasoactive Intestinal Peptide/*biosynthesis
MH  - Young Adult
OTO - NOTNLM
OT  - Crohn's disease
OT  - inflammation
OT  - neuro-immune interactions
OT  - ulcerative colitis
EDAT- 2018/11/09 06:00
MHDA- 2020/01/23 06:00
CRDT- 2018/11/09 06:00
PHST- 2018/05/03 00:00 [received]
PHST- 2018/09/24 00:00 [revised]
PHST- 2018/10/07 00:00 [accepted]
PHST- 2018/11/09 06:00 [pubmed]
PHST- 2020/01/23 06:00 [medline]
PHST- 2018/11/09 06:00 [entrez]
AID - 10.1111/nmo.13503 [doi]
PST - ppublish
SO  - Neurogastroenterol Motil. 2019 Mar;31(3):e13503. doi: 10.1111/nmo.13503. Epub 
      2018 Nov 8.