PMID- 30408082
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190610
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 11
DP  - 2018
TI  - Characterization of sensory neuronal subtypes innervating mouse tongue.
PG  - e0207069
LID - 10.1371/journal.pone.0207069 [doi]
LID - e0207069
AB  - The tongue is uniquely exposed to water-soluble environmental chemicals that may 
      lead to injury or tumorigenesis. However, comparatively little research has 
      focused on the molecular and functional organization of trigeminal ganglia (TG) 
      afferent neurons innervating the tongue. The current study identified and 
      characterized lingual sensory neurons based on a neuronal subtype classification 
      previously characterized in the dorsal root ganglion (DRG) neurons. We employed 
      immunohistochemistry on transgenic reporter mouse lines as well as single-cell 
      PCR of known markers of neuronal subtypes to characterize neuronal subtypes 
      innervating the tongue. Markers expressed in retrogradely labeled TG neurons were 
      evaluated for the proportion of neurons expressing each marker, intensity of 
      expression, and overlapping genes. We found that tongue-innervating sensory 
      neurons primarily expressed CGRP, TRPV1, TrkC, 5HT3A and Parvalbumin. These 
      markers correspond to peptidergic and a subgroup of non-peptidergic 
      C-nociceptors, peptidergic A nociceptors, proprioceptors and myelinated 
      low-threshold mechanoreceptors (LTMRs). Interestingly, as reported previously, we 
      also found several differences between TG and DRG neurons indicating the need for 
      single-cell sequencing of neuronal types based on tissue type within all TG as 
      well as DRG neurons.
FAU - Wu, Ping
AU  - Wu P
AUID- ORCID: 0000-0001-6372-9489
AD  - Department of Endodontics, University of Texas Health at San Antonio, San 
      Antonio, TX, United States of America.
FAU - Arris, Dominic
AU  - Arris D
AD  - Department of Endodontics, University of Texas Health at San Antonio, San 
      Antonio, TX, United States of America.
FAU - Grayson, Max
AU  - Grayson M
AD  - Department of Endodontics, University of Texas Health at San Antonio, San 
      Antonio, TX, United States of America.
FAU - Hung, Chia-Nung
AU  - Hung CN
AD  - Department of Molecular Medicine, University of Texas Health at San Antonio, San 
      Antonio, TX, United States of America.
AD  - Department of Life Science, Tunghai University, Taichung, Taiwan.
FAU - Ruparel, Shivani
AU  - Ruparel S
AD  - Department of Endodontics, University of Texas Health at San Antonio, San 
      Antonio, TX, United States of America.
LA  - eng
GR  - R01 DE027223/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20181108
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Male
MH  - Mice
MH  - Sensory Receptor Cells/*cytology/metabolism
MH  - Tongue/*innervation
PMC - PMC6224080
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/11/09 06:00
MHDA- 2019/04/16 06:00
PMCR- 2018/11/08
CRDT- 2018/11/09 06:00
PHST- 2018/08/06 00:00 [received]
PHST- 2018/10/24 00:00 [accepted]
PHST- 2018/11/09 06:00 [entrez]
PHST- 2018/11/09 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2018/11/08 00:00 [pmc-release]
AID - PONE-D-18-23177 [pii]
AID - 10.1371/journal.pone.0207069 [doi]
PST - epublish
SO  - PLoS One. 2018 Nov 8;13(11):e0207069. doi: 10.1371/journal.pone.0207069. 
      eCollection 2018.