PMID- 30408337
OWN - NLM
STAT- MEDLINE
DCOM- 20190820
LR  - 20190820
IS  - 1616-5195 (Electronic)
IS  - 1616-5187 (Linking)
VI  - 18
IP  - 12
DP  - 2018 Dec
TI  - Silk-Based Antimicrobial Polymers as a New Platform to Design Drug-Free Materials 
      to Impede Microbial Infections.
PG  - e1800262
LID - 10.1002/mabi.201800262 [doi]
AB  - Surgical site infections (SSI) represent a serious health problem that occur 
      after invasive surgery, thus new antimicrobial biomaterials able to prevent SSI 
      are needed. Silks are natural biopolymers with excellent biocompatibility, low 
      immunogenicity and controllable biodegradability. Spider silk-based materials can 
      be bioengineered and functionalized with specific peptides, such as antimicrobial 
      peptides, creating innovative polymers. Herein, we explored new drug-free 
      multifunctional silk films with antimicrobial properties, specifically tailored 
      to hamper microbial infections. Different spider silk domains derived from the 
      dragline sequence of the spider Nephila clavipes (6mer and 15mer, 27 and 41 kDa 
      proteins, respectively) were fused with the two antimicrobial peptides, Hepcidin 
      (Hep) and Human Neutrophil peptide 1 (HNP1). The self-assembly features of the 
      spider silk domains (beta-sheets) were maintained after functionalization. The 
      bioengineered 6mer-HNP1 protein demonstrated inhibitory effects against microbial 
      pathogens. Silk-based films with 6mer-HNP1 and different contents of silk fibroin 
      (SF) significantly reduced bacterial adhesion and biofilm formation, whereas 
      higher bacterial counts were found on the films prepared with 6mer or SF alone. 
      The silk-based films showed no cytotoxic effects on human foreskin fibroblasts. 
      The positive cellular response, together with structural and antimicrobial 
      properties, highlight the potential of these multifunctional silk-based films as 
      new materials for preventing SSI.
CI  - (c) 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Franco, Albina R
AU  - Franco AR
AUID- ORCID: 0000-0001-8974-8354
AD  - 3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables 
      and Biomimetics, University of Minho, Headquarters of the European Institute of 
      Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de 
      Ciencia e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimaraes, 
      Portugal.
AD  - ICVS/3B's - PT Government Associate Laboratory, 4805 Braga/Guimaraes, Portugal.
FAU - Palma Kimmerling, Erica
AU  - Palma Kimmerling E
AD  - Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
FAU - Silva, Carla
AU  - Silva C
AUID- ORCID: 0000-0001-6252-8693
AD  - 3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables 
      and Biomimetics, University of Minho, Headquarters of the European Institute of 
      Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de 
      Ciencia e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimaraes, 
      Portugal.
AD  - ICVS/3B's - PT Government Associate Laboratory, 4805 Braga/Guimaraes, Portugal.
FAU - Rodrigues, Fernando J
AU  - Rodrigues FJ
AD  - ICVS/3B's - PT Government Associate Laboratory, 4805 Braga/Guimaraes, Portugal.
AD  - Life and Health Sciences Research Institute, School of Health Sciences, 
      University of Minho, 4805 Braga, Portugal.
FAU - Leonor, Isabel B
AU  - Leonor IB
AUID- ORCID: 0000-0002-4319-4883
AD  - 3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables 
      and Biomimetics, University of Minho, Headquarters of the European Institute of 
      Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de 
      Ciencia e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimaraes, 
      Portugal.
AD  - ICVS/3B's - PT Government Associate Laboratory, 4805 Braga/Guimaraes, Portugal.
FAU - Reis, Rui L
AU  - Reis RL
AD  - 3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables 
      and Biomimetics, University of Minho, Headquarters of the European Institute of 
      Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de 
      Ciencia e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimaraes, 
      Portugal.
AD  - ICVS/3B's - PT Government Associate Laboratory, 4805 Braga/Guimaraes, Portugal.
AD  - The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at 
      University of Minho, Avepark, 4805-017, Barco, Guimaraes, Portugal.
FAU - Kaplan, David L
AU  - Kaplan DL
AD  - Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181108
PL  - Germany
TA  - Macromol Biosci
JT  - Macromolecular bioscience
JID - 101135941
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Hepcidins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (alpha-Defensins)
RN  - 0 (human neutrophil peptide 1)
RN  - 9007-76-5 (Fibroins)
MH  - Animals
MH  - Anti-Infective Agents/*chemistry/metabolism/pharmacology
MH  - Bacterial Adhesion/drug effects
MH  - Biocompatible Materials/*chemistry/metabolism/pharmacology
MH  - Biofilms/drug effects/growth & development
MH  - Candida albicans/drug effects/growth & development
MH  - Cell Line
MH  - Cell Survival
MH  - Fibroblasts/cytology/drug effects
MH  - Fibroins/biosynthesis/*chemistry/genetics/pharmacology
MH  - Gene Expression
MH  - Gram-Negative Bacteria/drug effects/growth & development
MH  - Gram-Positive Bacteria/drug effects/growth & development
MH  - Hepcidins/*biosynthesis/genetics/pharmacology
MH  - Humans
MH  - Microbial Viability/drug effects
MH  - Plasmids/chemistry/metabolism
MH  - Polymerization
MH  - Protein Conformation, beta-Strand
MH  - Recombinant Fusion Proteins/biosynthesis/*chemistry/genetics/pharmacology
MH  - Spiders/physiology
MH  - Surgical Wound Infection/prevention & control
MH  - Sutures/microbiology
MH  - alpha-Defensins/*biosynthesis/genetics/pharmacology
OTO - NOTNLM
OT  - antimicrobial peptides
OT  - bacterial adhesion
OT  - biocompatibility
OT  - bioengineered silk proteins
OT  - drug-free polymers
EDAT- 2018/11/09 06:00
MHDA- 2019/08/21 06:00
CRDT- 2018/11/09 06:00
PHST- 2018/07/11 00:00 [received]
PHST- 2018/10/03 00:00 [revised]
PHST- 2018/11/09 06:00 [pubmed]
PHST- 2019/08/21 06:00 [medline]
PHST- 2018/11/09 06:00 [entrez]
AID - 10.1002/mabi.201800262 [doi]
PST - ppublish
SO  - Macromol Biosci. 2018 Dec;18(12):e1800262. doi: 10.1002/mabi.201800262. Epub 2018 
      Nov 8.