PMID- 30409859
OWN - NLM
STAT- MEDLINE
DCOM- 20190508
LR  - 20200225
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 362
IP  - 6415
DP  - 2018 Nov 9
TI  - Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast 
      cells via microvesicles.
LID - 10.1126/science.aao0666 [doi]
LID - eaao0666
AB  - Anaphylactic reactions are triggered when allergens enter the blood circulation 
      and activate immunoglobulin E (IgE)-sensitized mast cells (MCs), causing systemic 
      discharge of prestored proinflammatory mediators. As MCs are extravascular, how 
      they perceive circulating allergens remains a conundrum. Here, we describe the 
      existence of a CD301b(+) perivascular dendritic cell (DC) subset that 
      continuously samples blood and relays antigens to neighboring MCs, which 
      vigorously degranulate and trigger anaphylaxis. DC antigen transfer involves the 
      active discharge of surface-associated antigens on 0.5- to 1.0-micrometer 
      microvesicles (MVs) generated by vacuolar protein sorting 4 (VPS4). Antigen 
      sharing by DCs is not limited to MCs, as neighboring DCs also acquire 
      antigen-bearing MVs. This capacity of DCs to distribute antigen-bearing MVs to 
      various immune cells in the perivascular space potentiates inflammatory and 
      immune responses to blood-borne antigens.
CI  - Copyright (c) 2018 The Authors, some rights reserved; exclusive licensee American 
      Association for the Advancement of Science. No claim to original U.S. Government 
      Works.
FAU - Choi, Hae Woong
AU  - Choi HW
AUID- ORCID: 0000-0001-9372-1875
AD  - Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA. 
      haewoong.choi@duke.edu.
FAU - Suwanpradid, Jutamas
AU  - Suwanpradid J
AD  - Department of Dermatology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Kim, Il Hwan
AU  - Kim IH
AD  - Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, 
      Durham, NC 27710, USA.
FAU - Staats, Herman F
AU  - Staats HF
AUID- ORCID: 0000-0003-1039-1087
AD  - Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA.
AD  - Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
AD  - Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, 
      USA.
FAU - Haniffa, Muzlifah
AU  - Haniffa M
AUID- ORCID: 0000-0002-3927-2084
AD  - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 
      4HH, UK.
AD  - Department of Dermatology, Newcastle upon Tyne NHS Trust, Newcastle upon Tyne NE1 
      4LP, UK.
FAU - MacLeod, Amanda S
AU  - MacLeod AS
AD  - Department of Dermatology, Duke University Medical Center, Durham, NC 27710, USA.
AD  - Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Abraham, Soman N
AU  - Abraham SN
AUID- ORCID: 0000-0002-1662-2096
AD  - Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA.
AD  - Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
AD  - Department of Molecular Genetics and Microbiology, Duke University Medical 
      Center, Durham, NC 27710, USA.
AD  - Program in Emerging Infectious Diseases, Duke-National University of Singapore, 
      Singapore 169857, Singapore.
LA  - eng
GR  - R01 AI096305/AI/NIAID NIH HHS/United States
GR  - R21 AI128727/AI/NIAID NIH HHS/United States
GR  - R56 DK095198/DK/NIDDK NIH HHS/United States
GR  - U01 AI082107/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Allergens)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Lectins, C-Type)
RN  - 0 (MGL2 protein, mouse)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.6 (Vps4b protein, mouse)
SB  - IM
CIN - Science. 2018 Nov 9;362(6415):640-641. PMID: 30409871
CIN - Immunity. 2018 Dec 18;49(6):999-1001. PMID: 30566890
MH  - ATPases Associated with Diverse Cellular Activities/immunology
MH  - Allergens/*immunology
MH  - Anaphylaxis/*immunology
MH  - Animals
MH  - Blood Vessels/immunology
MH  - Cell-Derived Microparticles/*immunology
MH  - Dendritic Cells/*immunology
MH  - Endosomal Sorting Complexes Required for Transport/immunology
MH  - Female
MH  - Humans
MH  - Lectins, C-Type/immunology
MH  - Male
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Primary Cell Culture
MH  - Skin/*immunology
PMC - PMC6376486
MID - NIHMS1006357
EDAT- 2018/11/10 06:00
MHDA- 2019/05/09 06:00
PMCR- 2019/02/15
CRDT- 2018/11/10 06:00
PHST- 2017/06/10 00:00 [received]
PHST- 2018/04/20 00:00 [revised]
PHST- 2018/09/12 00:00 [accepted]
PHST- 2018/11/10 06:00 [entrez]
PHST- 2018/11/10 06:00 [pubmed]
PHST- 2019/05/09 06:00 [medline]
PHST- 2019/02/15 00:00 [pmc-release]
AID - 362/6415/eaao0666 [pii]
AID - 10.1126/science.aao0666 [doi]
PST - ppublish
SO  - Science. 2018 Nov 9;362(6415):eaao0666. doi: 10.1126/science.aao0666.