PMID- 30412637
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 11
DP  - 2018
TI  - Comparison of proinsulin and C-peptide secretion in healthy versus long-standing 
      type 1 diabetes mellitus cohorts: A pilot study.
PG  - e0207065
LID - 10.1371/journal.pone.0207065 [doi]
LID - e0207065
AB  - AIMS: Increased proinsulin (PI) compared to C-peptide (CP) concentrations have 
      been reported, both prior to type 1 diabetes mellitus (T1D) onset, as well as 
      early in disease. In this pilot study, we sought to define the normal PI 
      secretion in a healthy cohort and compare this to a local T1D cohort and a 
      separate well-defined nationally representative T1D cohort with measurable CP. 
      METHODS: Thirteen healthy subjects and 12 T1D subjects with T1D >3 years from the 
      local T1D cohort completed mixed meal tolerance tests (MMTT) with PI and CP 
      measured over 90 and 240 minutes. The change in CP (maximum versus baseline, DeltaCP) 
      during MMTT in the T1D Exchange T1D cohort was stratified according to 
      non-fasting PI concentrations, based on a fasting PI threshold, as defined by the 
      healthy control group. RESULTS: The maximum fasting PI in the control group was 6 
      pmol/L. Individuals from the T1D Exchange with a non-fasting PI >/= 6 pmol/L had a 
      lower DeltaCP during a MMTT, compared to those with a PI < 6 pmol/L. While only three 
      individuals from the local T1D cohort had measurable CP and PI during the MMTT, 
      those with a greater DeltaCP had lower PI secretion. CONCLUSION: While all T1D 
      subjects from the T1D Exchange secreted measurable non-fasting PI, those with a 
      greater non-fasting PI demonstrated a decrease in DeltaCP during the MMTT. PI may be 
      preferentially secreted compared to CP in some individuals with long standing 
      T1D.
FAU - Sullivan, Catherine A
AU  - Sullivan CA
AUID- ORCID: 0000-0003-1035-5828
AD  - Section of Endocrinology, Diabetes and Nutrition, Boston University School of 
      Medicine, Boston, MA, United States of America.
FAU - Cacicedo, Jose M
AU  - Cacicedo JM
AD  - Section of Endocrinology, Diabetes and Nutrition, Boston University School of 
      Medicine, Boston, MA, United States of America.
FAU - Rajendran, Iniya
AU  - Rajendran I
AD  - School of Public Health, Boston University, Boston, MA, United States of America.
FAU - Steenkamp, Devin W
AU  - Steenkamp DW
AD  - Section of Endocrinology, Diabetes and Nutrition, Boston University School of 
      Medicine, Boston, MA, United States of America.
LA  - eng
GR  - UL1 TR001430/TR/NCATS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20181109
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Blood Glucose)
RN  - 0 (C-Peptide)
RN  - 9035-68-1 (Proinsulin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Blood Glucose/metabolism
MH  - C-Peptide/*metabolism
MH  - Case-Control Studies
MH  - Cohort Studies
MH  - Diabetes Mellitus, Type 1/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pilot Projects
MH  - Proinsulin/*metabolism
MH  - Young Adult
PMC - PMC6226191
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/11/10 06:00
MHDA- 2019/04/16 06:00
PMCR- 2018/11/09
CRDT- 2018/11/10 06:00
PHST- 2018/09/07 00:00 [received]
PHST- 2018/10/24 00:00 [accepted]
PHST- 2018/11/10 06:00 [entrez]
PHST- 2018/11/10 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2018/11/09 00:00 [pmc-release]
AID - PONE-D-18-26317 [pii]
AID - 10.1371/journal.pone.0207065 [doi]
PST - epublish
SO  - PLoS One. 2018 Nov 9;13(11):e0207065. doi: 10.1371/journal.pone.0207065. 
      eCollection 2018.