PMID- 30417091
OWN - NLM
STAT- MEDLINE
DCOM- 20190911
LR  - 20190911
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 4
IP  - 11
DP  - 2018 Nov
TI  - A tyrosine sulfation-dependent HLA-I modification identifies memory B cells and 
      plasma cells.
PG  - eaar7653
LID - 10.1126/sciadv.aar7653 [doi]
LID - eaar7653
AB  - Memory B cells and plasma cells are antigen-experienced cells tasked with the 
      maintenance of humoral protection. Despite these prominent functions, definitive 
      cell surface markers have not been identified for these cells. We report here the 
      isolation and characterization of the monoclonal variable lymphocyte receptor B 
      (VLRB) N8 antibody from the evolutionarily distant sea lamprey that specifically 
      recognizes memory B cells and plasma cells in humans. Unexpectedly, we determined 
      that VLRB N8 recognizes the human leukocyte antigen-I (HLA-I) antigen in a 
      tyrosine sulfation-dependent manner. Furthermore, we observed increased binding 
      of VLRB N8 to memory B cells in individuals with autoimmune disorders multiple 
      sclerosis and systemic lupus erythematosus. Our study indicates that lamprey VLR 
      antibodies uniquely recognize a memory B cell- and plasma cell-specific 
      posttranslational modification of HLA-I, the expression of which is up-regulated 
      during B cell activation.
FAU - Chan, Justin T H
AU  - Chan JTH
AUID- ORCID: 0000-0003-4271-0177
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
FAU - Liu, Yanling
AU  - Liu Y
AUID- ORCID: 0000-0001-6119-6287
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
FAU - Khan, Srijit
AU  - Khan S
AUID- ORCID: 0000-0002-2934-7687
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
FAU - St-Germain, Jonathan R
AU  - St-Germain JR
AUID- ORCID: 0000-0003-1640-6964
AD  - Department of Molecular Genetics, Hospital for Sick Children, Toronto, ON, 
      Canada.
FAU - Zou, Chunxia
AU  - Zou C
AD  - Alberta Glycomics Centre and Department of Chemistry, University of Alberta, 
      Edmonton, AB, Canada.
FAU - Leung, Leslie Y T
AU  - Leung LYT
AUID- ORCID: 0000-0002-4883-582X
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
FAU - Yang, Judi
AU  - Yang J
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
FAU - Shi, Mengyao
AU  - Shi M
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
FAU - Grunebaum, Eyal
AU  - Grunebaum E
AD  - Division of Immunology and Allergy, Hospital for Sick Children and University of 
      Toronto, Toronto, ON, Canada.
FAU - Campisi, Paolo
AU  - Campisi P
AD  - Department of Otolaryngology-Head and Neck Surgery, Hospital for Sick Children 
      and University of Toronto, Toronto, ON, Canada.
FAU - Propst, Evan J
AU  - Propst EJ
AD  - Department of Otolaryngology-Head and Neck Surgery, Hospital for Sick Children 
      and University of Toronto, Toronto, ON, Canada.
FAU - Holler, Theresa
AU  - Holler T
AD  - Department of Otolaryngology-Head and Neck Surgery, Hospital for Sick Children 
      and University of Toronto, Toronto, ON, Canada.
FAU - Bar-Or, Amit
AU  - Bar-Or A
AD  - Department of Neurology, University of Pennsylvania School of Medicine, 
      Philadelphia, PA, USA.
FAU - Wither, Joan E
AU  - Wither JE
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
FAU - Cairo, Christopher W
AU  - Cairo CW
AUID- ORCID: 0000-0003-3363-8708
AD  - Alberta Glycomics Centre and Department of Chemistry, University of Alberta, 
      Edmonton, AB, Canada.
FAU - Moran, Michael F
AU  - Moran MF
AD  - Department of Molecular Genetics, Hospital for Sick Children, Toronto, ON, 
      Canada.
FAU - Palazzo, Alexander F
AU  - Palazzo AF
AUID- ORCID: 0000-0002-9700-1995
AD  - Department of Biochemistry, University of Toronto, Toronto, ON, Canada.
FAU - Cooper, Max D
AU  - Cooper MD
AD  - Department of Pathology and Laboratory Medicine and Emory Vaccine Center, Emory 
      University School of Medicine, Atlanta, GA, USA.
FAU - Ehrhardt, Gotz R A
AU  - Ehrhardt GRA
AUID- ORCID: 0000-0002-5868-5191
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada.
LA  - eng
GR  - R01 AI072435/AI/NIAID NIH HHS/United States
GR  - U19 AI096187/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20181107
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Receptors, Antigen)
RN  - 29166358BF (tyrosine O-sulfate)
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/blood/*immunology
MH  - B-Lymphocytes/*immunology/metabolism
MH  - Cells, Cultured
MH  - Histocompatibility Antigens Class I/*immunology/metabolism
MH  - Humans
MH  - Immunoglobulin Variable Region/immunology/metabolism
MH  - Immunologic Memory/*immunology
MH  - Lampreys/immunology
MH  - Lupus Erythematosus, Systemic/immunology/metabolism
MH  - Multiple Sclerosis/immunology/metabolism
MH  - Plasma Cells/*immunology/metabolism
MH  - Receptors, Antigen/*immunology/metabolism
MH  - Tyrosine/*analogs & derivatives/chemistry
PMC - PMC6221509
EDAT- 2018/11/13 06:00
MHDA- 2019/09/12 06:00
PMCR- 2018/11/07
CRDT- 2018/11/13 06:00
PHST- 2017/12/14 00:00 [received]
PHST- 2018/10/12 00:00 [accepted]
PHST- 2018/11/13 06:00 [entrez]
PHST- 2018/11/13 06:00 [pubmed]
PHST- 2019/09/12 06:00 [medline]
PHST- 2018/11/07 00:00 [pmc-release]
AID - aar7653 [pii]
AID - 10.1126/sciadv.aar7653 [doi]
PST - epublish
SO  - Sci Adv. 2018 Nov 7;4(11):eaar7653. doi: 10.1126/sciadv.aar7653. eCollection 2018 
      Nov.