PMID- 30417207
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20210826
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 1
DP  - 2019 Feb
TI  - Leptin is a direct transcriptional target of EGR1 in human breast cancer cells.
PG  - 317-324
LID - 10.1007/s11033-018-4474-3 [doi]
AB  - Leptin is a cytokine that regulates energy metabolism. Leptin can promote breast 
      cancer progression in obese women. However, the mechanism of regulation of leptin 
      expression in breast cancer cells is unclear. Tumor necrosis factor-alpha (TNF-alpha) 
      stimulated the transcription of the leptin gene. Using mutant promoter 
      constructs, we demonstrated that the EGR1-binding motif in the proximal region of 
      the leptin gene is required for leptin transcription by TNF-alpha. Forced expression 
      of EGR1 stimulated leptin promoter activity, whereas silencing of EGR1 by RNA 
      interference reduced TNF-alpha-induced leptin protein accumulation. The ERK1/2 
      pathway contributed to the expression of EGR1 and leptin by TNF-alpha. Our results 
      suggest that EGR1 targets the leptin gene in response to TNF-alpha stimulation in 
      breast cancer cells.
FAU - Kim, JuHwan
AU  - Kim J
AD  - Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Jung, Euitaek
AU  - Jung E
AD  - Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Choi, Jihye
AU  - Choi J
AD  - Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Min, Dong Yeong
AU  - Min DY
AD  - Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Lee, Young Han
AU  - Lee YH
AD  - Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk 
      University, Seoul, 05029, Republic of Korea.
AD  - Cancer and Metabolism Institute, Konkuk University, Seoul, 05029, Republic of 
      Korea.
FAU - Shin, Soon Young
AU  - Shin SY
AUID- ORCID: 0000-0002-1075-0011
AD  - Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk 
      University, Seoul, 05029, Republic of Korea. shinsy@konkuk.ac.kr.
AD  - Cancer and Metabolism Institute, Konkuk University, Seoul, 05029, Republic of 
      Korea. shinsy@konkuk.ac.kr.
LA  - eng
GR  - 2016R1A2B4008570/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20181111
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EGR1 protein, human)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Leptin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Binding Sites
MH  - Breast Neoplasms/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/genetics
MH  - Early Growth Response Protein 1/genetics/*physiology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Leptin/genetics/*physiology
MH  - MAP Kinase Signaling System/genetics/physiology
MH  - Promoter Regions, Genetic/drug effects/genetics
MH  - RNA, Messenger/metabolism
MH  - Transcription Factors/genetics
MH  - Transcription, Genetic/drug effects
MH  - Transcriptional Activation/drug effects
MH  - Tumor Necrosis Factor-alpha/genetics/physiology
OTO - NOTNLM
OT  - Breast cancer
OT  - EGR1
OT  - ERK1/2
OT  - Leptin
OT  - Tissue necrosis factor alpha
EDAT- 2018/11/13 06:00
MHDA- 2019/07/02 06:00
CRDT- 2018/11/13 06:00
PHST- 2018/09/04 00:00 [received]
PHST- 2018/11/07 00:00 [accepted]
PHST- 2018/11/13 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
PHST- 2018/11/13 06:00 [entrez]
AID - 10.1007/s11033-018-4474-3 [pii]
AID - 10.1007/s11033-018-4474-3 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Feb;46(1):317-324. doi: 10.1007/s11033-018-4474-3. Epub 2018 
      Nov 11.