PMID- 30419031
OWN - NLM
STAT- MEDLINE
DCOM- 20190409
LR  - 20191210
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 11
DP  - 2018
TI  - Development and validation of a HPLC/FLD method combined with online 
      derivatization for the simple and simultaneous determination of trace amino acids 
      and alkyl amines in continental and marine aerosols.
PG  - e0206488
LID - 10.1371/journal.pone.0206488 [doi]
LID - e0206488
AB  - A method was developed for simultaneous determination of 15 amino acids and 7 
      alkyl amines. The method was based on the employment of high performance liquid 
      chromatography/fluorescence detection and online derivatization with 
      o-phthaldiadehyde. The 22 derivatives were separated within 30 min including the 
      equilibration time and detected by a fluorescence detector at an excitation 
      wavelength of 230 nm and emission wavelength of 450 nm. The analysis procedure 
      was satisfactorily validated by the reproducibility, recovery, linearity and 
      detection limit of the analytes. The relative standard deviations (RSDs) of 
      retention time and peak area for individual amino acids and alkyl amines were 
      consistently less than 0.30% and 2.35%, respectively. Good recovery values 
      ranging from 70% to 109% were obtained. The proposed method showed good linearity 
      (R2>/=0.99) in the range of 0.125-125 muM/L for amino acids and 2.5-5000 ng/L for 
      alkyl amines. The detection limit ranged from 0.13 pM to 0.37 pM for individual 
      amino acids and from 0.9 ng to 7.2 ng for individual alkyl amines. The developed 
      and validated method was successfully applied to the quantitative analysis of 
      amino acids and alkyl amines in continental and marine aerosols in China. Among 
      the identified organic nitrogen compounds, 7 amino acids and 6 alkyl amines were 
      detected in every aerosol sample. Glycine was the dominant amino acid, with the 
      average of 130.93 pmol/m3 (accounting for 83% of the total amino acids) and 
      137.22 pmol/m3 (accounting for 66% of the total amino acids) in continental and 
      marine aerosols in China, respectively. Methylamine and ethanolamine were the 
      most abundant alkyl amines, contributing 87% and 64% to the total alkyl amines in 
      continental and marine aerosols in China, respectively. This work provided an 
      accurate, sensitive and simple method to determine simultaneously amino acids and 
      alkyl amines, and applied the proposed method to the first investigation of amino 
      acids in Shanghai and amino acids and alkyl amines in Huaniao Island in China. 
      The finding of considerable amino acids and alkyl amines in continental and 
      marine aerosols may exert significant implications on nitrogen cycling and 
      atmospheric chemistry.
FAU - Huang, Xiongfeng
AU  - Huang X
AD  - Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), 
      Department of Environmental Science and Engineering, Fudan University, Shanghai, 
      China.
AD  - State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, 
      China.
FAU - Kao, Shuh-Ji
AU  - Kao SJ
AD  - State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, 
      China.
FAU - Lin, Jing
AU  - Lin J
AD  - Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), 
      Department of Environmental Science and Engineering, Fudan University, Shanghai, 
      China.
AD  - Fuzhou Research Academy of Environmental Science, Fuzhou, China.
FAU - Qin, Xiaofei
AU  - Qin X
AD  - Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), 
      Department of Environmental Science and Engineering, Fudan University, Shanghai, 
      China.
FAU - Deng, Congrui
AU  - Deng C
AUID- ORCID: 0000-0002-2165-5431
AD  - Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), 
      Department of Environmental Science and Engineering, Fudan University, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20181112
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Aerosols)
RN  - 0 (Amines)
RN  - 0 (Amino Acids)
SB  - IM
MH  - Aerosols
MH  - Alkylation
MH  - Amines/*analysis/*chemistry
MH  - Amino Acids/*analysis/chemistry
MH  - Analytic Sample Preparation Methods/*methods
MH  - Atmosphere/chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Limit of Detection
MH  - Quality Control
MH  - Spectrometry, Fluorescence/*methods
MH  - Time Factors
PMC - PMC6231614
COIS- The authors have declared that no competing interests exist. There are no any 
      ethical/legal conflicts involved in the article.
EDAT- 2018/11/13 06:00
MHDA- 2019/04/10 06:00
PMCR- 2018/11/12
CRDT- 2018/11/13 06:00
PHST- 2017/08/08 00:00 [received]
PHST- 2018/10/15 00:00 [accepted]
PHST- 2018/11/13 06:00 [entrez]
PHST- 2018/11/13 06:00 [pubmed]
PHST- 2019/04/10 06:00 [medline]
PHST- 2018/11/12 00:00 [pmc-release]
AID - PONE-D-17-29387 [pii]
AID - 10.1371/journal.pone.0206488 [doi]
PST - epublish
SO  - PLoS One. 2018 Nov 12;13(11):e0206488. doi: 10.1371/journal.pone.0206488. 
      eCollection 2018.