PMID- 30419326
OWN - NLM
STAT- MEDLINE
DCOM- 20190410
LR  - 20190410
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 123
DP  - 2019 Feb 15
TI  - Understanding the nano-bio interactions using real-time surface plasmon resonance 
      tool.
PG  - 97-107
LID - S0141-8130(18)33030-7 [pii]
LID - 10.1016/j.ijbiomac.2018.11.039 [doi]
AB  - Our current understanding of the biophysicochemical interactions at nano-bio 
      interfaces is still very limited. Surface plasmon resonance (SPR) is a powerful 
      tool for understanding the real-time kinetics of protein binding on the surface 
      of nanoparticles (NPs) but has been least exploited for this purpose. In this 
      study, we demonstrated the interaction of negatively charged poly 
      lactic-co-glycolic acid (PLGA) NPs and positively charged chitosan 
      oligosaccharide (COS)-coated PLGA NPs with two model proteins, namely bovine 
      serum albumin (BSA) and hen egg white lysozyme (LYZ), at the physiological pH of 
      7.4. Various biophysical characterization techniques were employed to elucidate 
      the influence of surface charge of NPs on protein interaction. SPR investigations 
      revealed the binding affinity and binding kinetics involved in 
      nanoparticle-protein interactions. These results confirmed that the affinity of 
      both types of NPs towards positively charged LYZ was much greater than that for 
      negatively charged BSA, which was also in accordance with the results of the 
      adsorption studies. Our results demonstrate that the surface properties of the 
      interacting species play a dominant role during protein-nanoparticle 
      interactions, apart from the net charge on their individual surfaces. The 
      information obtained from this study adds significant value to the 
      biophysicochemical toolbox for characterization of nano-bio interactions.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Atale, Sonal S
AU  - Atale SS
AD  - Department of Pharmaceutical Sciences and Technology, Institute of Chemical 
      Technology, NP Marg, Matunga, Mumbai 400019, India.
FAU - Dyawanapelly, Sathish
AU  - Dyawanapelly S
AD  - Department of Chemical Engineering, Institute of Chemical Technology, Matunga, 
      Mumbai 400019, India.
FAU - Jagtap, Dhanashree D
AU  - Jagtap DD
AD  - Division of Structural Biology, National Institute for Research in Reproductive 
      Health (Indian Council of Medical Research), Jehangir Merwanji Street, Parel, 
      Mumbai 400012, India.
FAU - Jain, Ratnesh
AU  - Jain R
AD  - Department of Chemical Engineering, Institute of Chemical Technology, Matunga, 
      Mumbai 400019, India. Electronic address: rd.jain@ictmumbai.edu.in.
FAU - Dandekar, Prajakta
AU  - Dandekar P
AD  - Department of Pharmaceutical Sciences and Technology, Institute of Chemical 
      Technology, NP Marg, Matunga, Mumbai 400019, India. Electronic address: 
      pd.jain@ictmumbai.edu.in.
LA  - eng
PT  - Journal Article
DEP - 20181109
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Oligosaccharides)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.2.1.- (hen egg lysozyme)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Adsorption/drug effects
MH  - Chitosan/chemistry
MH  - Kinetics
MH  - Muramidase/chemistry
MH  - Nanoparticles/*chemistry
MH  - Oligosaccharides/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/chemistry
MH  - Protein Interaction Domains and Motifs/drug effects
MH  - Serum Albumin, Bovine/chemistry
MH  - Surface Plasmon Resonance/methods
MH  - Surface Properties/drug effects
OTO - NOTNLM
OT  - Protein-nanoparticles interaction
OT  - Surface charge
OT  - Surface plasmon resonance
EDAT- 2018/11/13 06:00
MHDA- 2019/04/11 06:00
CRDT- 2018/11/13 06:00
PHST- 2018/06/19 00:00 [received]
PHST- 2018/11/08 00:00 [revised]
PHST- 2018/11/08 00:00 [accepted]
PHST- 2018/11/13 06:00 [pubmed]
PHST- 2019/04/11 06:00 [medline]
PHST- 2018/11/13 06:00 [entrez]
AID - S0141-8130(18)33030-7 [pii]
AID - 10.1016/j.ijbiomac.2018.11.039 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Feb 15;123:97-107. doi: 10.1016/j.ijbiomac.2018.11.039. 
      Epub 2018 Nov 9.