PMID- 30419554
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 50
IP  - 6
DP  - 2018
TI  - MALAT1 Activates the P53 Signaling Pathway by Regulating MDM2 to Promote Ischemic 
      Stroke.
PG  - 2216-2228
LID - 10.1159/000495083 [doi]
AB  - BACKGROUND/AIMS: This study focused on evaluating the effect of MALAT1 and MDM2 
      on ischemic stroke through regulation of the p53 signaling pathway. MATERIALS: 
      Bioinformatics analysis was performed to identify abnormally expressed lncRNAs, 
      mRNAs and their associated pathways. Oxygen-glucose deprivation/reoxygenation 
      (OGD/R) in cells and middle cerebral artery occlusion/reperfusion (MCAO/R) in 
      mice were performed to simulate an ischemic stroke environment. Western blot and 
      qRT-PCR were used to examine lncRNA expression and mRNA levels. Fluorescence in 
      situ hybridization (FISH) LncRNA was used to locate mRNA. MTT and flow cytometry 
      were performed to examine cell proliferation and apoptosis. Finally, 
      immunohistochemistry was used to observe the expression of genes in vivo. 
      RESULTS: MALAT1 and MDM2, which exhibit strong expression in stroke tissues, were 
      subjected to bioinformatics analysis, and the p53 pathway was chosen for further 
      study. MALAT1, MDM2 and p53 signaling pathway-related proteins were all up 
      regulated in OGD/R cells. Furthermore, Malat1, Mdm2 and p53 pathway 
      related-proteins were also up regulated in MCAO/R mice. Both MALAT1 and MDM2 were 
      localized in the nuclei. Down regulation of MALAT1 and MDM2 enhanced cell 
      proliferation ability and reduced apoptosis, resulting in decreased infarct size 
      in MCAO/R brains. CONCLUSION: These results indicate that MALAT1/MDM2/p53 
      signaling pathway axis may provide more effective clinical therapeutic strategy 
      for patients with ischemic stroke.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Zhang, Ting
AU  - Zhang T
AD  - Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, Shanghai, China549832649@qq.com.
FAU - Wang, Hongmei
AU  - Wang H
AD  - Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, Shanghai, China.
FAU - Li, Qiang
AU  - Li Q
AD  - Department of Neurology, Shanghai Jiao Tong University Affillilated Ninth 
      People's Hospital, Shanghai, China.
FAU - Fu, Jianliang
AU  - Fu J
AD  - Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, Shanghai, China.
FAU - Huang, Jiankang
AU  - Huang J
AD  - Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, Shanghai, China.
FAU - Zhao, Yuwu
AU  - Zhao Y
AD  - Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20181112
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (MALAT1 long non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Brain/metabolism/pathology
MH  - Cell Hypoxia
MH  - Cell Proliferation
MH  - Endothelial Cells/cytology/metabolism
MH  - Glucose/deficiency
MH  - Humans
MH  - Infarction, Middle Cerebral Artery/complications/pathology
MH  - Mice
MH  - Oxygen/metabolism
MH  - Proto-Oncogene Proteins c-mdm2/antagonists & inhibitors/genetics/*metabolism
MH  - RNA Interference
MH  - RNA, Long Noncoding/antagonists & inhibitors/genetics/*metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Signal Transduction
MH  - Stroke/etiology/metabolism/pathology
MH  - Tumor Suppressor Protein p53/antagonists & inhibitors/genetics/*metabolism
MH  - bcl-2-Associated X Protein/metabolism
OTO - NOTNLM
OT  - Ischemic stroke
OT  - MALAT1
OT  - MDM2
OT  - P53 pathway
EDAT- 2018/11/13 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/11/13 06:00
PHST- 2018/07/10 00:00 [received]
PHST- 2018/11/05 00:00 [accepted]
PHST- 2018/11/13 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/11/13 06:00 [entrez]
AID - 000495083 [pii]
AID - 10.1159/000495083 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;50(6):2216-2228. doi: 10.1159/000495083. Epub 2018 Nov 
      12.