PMID- 30420053
OWN - NLM
STAT- MEDLINE
DCOM- 20190506
LR  - 20190506
IS  - 1557-9832 (Electronic)
IS  - 0272-2712 (Linking)
VI  - 38
IP  - 4
DP  - 2018 Dec
TI  - Human Leukocyte Antigen Typing by Next-Generation Sequencing.
PG  - 565-578
LID - S0272-2712(18)31178-8 [pii]
LID - 10.1016/j.cll.2018.07.006 [doi]
AB  - Human leukocyte antigen (HLA) allele ambiguities are the result of limitations of 
      current HLA typing methodologies. Ambiguities maybe due to polymorphisms in 
      unsequenced regions of HLA genes or cis/trans variants that cannot be 
      distinguished by Sanger sequencing. Next generation sequencing (NGS) can resolve 
      these two sources of ambiguity because the entire gene can be sequenced. 
      Commercially available HLA NGS genotyping kits enable laboratories to deliver 
      high-quality and unambiguous HLA typing results at an affordable cost. Third 
      generation sequencing technologies are poised to further improve sequencing 
      quality, shorten turn-around and library preparation times, as well as provide 
      full-gene phasing.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Profaizer, Tracie
AU  - Profaizer T
AD  - ARUP Institute for Clinical and Experimental Pathology, Department of Pathology, 
      University of Utah School of Medicine, 500 Chipeta Way, Salt Lake City, UT 84108, 
      USA. Electronic address: tracie.profaizer@aruplab.com.
FAU - Kumanovics, Attila
AU  - Kumanovics A
AD  - ARUP Institute for Clinical and Experimental Pathology, Department of Pathology, 
      University of Utah School of Medicine, 500 Chipeta Way, Salt Lake City, UT 84108, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20181005
PL  - United States
TA  - Clin Lab Med
JT  - Clinics in laboratory medicine
JID - 8100174
RN  - 0 (HLA Antigens)
SB  - IM
MH  - HLA Antigens/*genetics
MH  - *High-Throughput Nucleotide Sequencing
MH  - Histocompatibility Testing
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - *Sequence Analysis, DNA
OTO - NOTNLM
OT  - DNA sequencing
OT  - Human leukocyte antigen (HLA)
OT  - Next generation sequencing (NGS)
OT  - Polymerase chain reaction (PCR)
EDAT- 2018/11/14 06:00
MHDA- 2019/05/07 06:00
CRDT- 2018/11/14 06:00
PHST- 2018/11/14 06:00 [entrez]
PHST- 2018/11/14 06:00 [pubmed]
PHST- 2019/05/07 06:00 [medline]
AID - S0272-2712(18)31178-8 [pii]
AID - 10.1016/j.cll.2018.07.006 [doi]
PST - ppublish
SO  - Clin Lab Med. 2018 Dec;38(4):565-578. doi: 10.1016/j.cll.2018.07.006. Epub 2018 
      Oct 5.