PMID- 30427204
OWN - NLM
STAT- MEDLINE
DCOM- 20190503
LR  - 20190503
IS  - 1466-1799 (Electronic)
IS  - 0007-1668 (Linking)
VI  - 60
IP  - 1
DP  - 2019 Feb
TI  - Forkhead box O3 promotes cell proliferation and inhibits myotube differentiation 
      in chicken myoblast cells.
PG  - 23-30
LID - 10.1080/00071668.2018.1547362 [doi]
AB  - 1. In the poultry industry, growth performance is important due to its effects on 
      economic value. Much effort has been put forth to achieve introgression of 
      specific genes and DNA markers related to muscle proliferation and 
      differentiation in selective breeding approaches. 2. This study investigated the 
      biological functions of the gene Forkhead box O3 (FOXO3) during myogenic 
      differentiation in chicken myoblast cells. FOXO3 was downregulated in primary 
      chicken myoblast (pCM) cells by the piggyBac transposon-mediated microRNA (miRNA) 
      knock-down (KD) system. 3. The pCM cells that were stably integrated into the 
      FOXO3 KD expression vector showed significant downregulation of FOXO3 protein and 
      mRNA levels. Expression levels of paired box protein Pax7 (Pax7) and target genes 
      such as CCAAT/enhancer binding protein beta and serum response element decreased 
      in FOXO3 KD pCM cells. In addition, in the undifferentiated myoblast stage, there 
      were no significant differences in cell morphology; however, proliferation rate 
      in FOXO3 KD pCM cells was significantly lower during d 4 and 5 of in vitro 
      culture. By contrast, when myotube differentiation was induced, FOXO3 KD pCM 
      cells exhibited rapid initiation of myotube formation, higher expression of 
      myogenin and desmin as myogenic indicators and a further differentiated phenotype 
      than observed in regular pCM cells. 4. These results demonstrated that FOXO3 
      promotes cell proliferation and inhibits myotube differentiation in chicken 
      myoblast cells. Therefore, the regulation of FOXO3 could be applied to improve 
      muscle differentiation in commercial poultry.
FAU - Lee, J H
AU  - Lee JH
AD  - a Graduate School of International Agricultural Technology , Seoul National 
      University , Pyeongchang-gun , Korea.
AD  - b Institute of Green-Bio Science and Technology , Seoul National University , 
      Pyeongchang-gun , Korea.
FAU - Park, J-W
AU  - Park JW
AD  - b Institute of Green-Bio Science and Technology , Seoul National University , 
      Pyeongchang-gun , Korea.
FAU - Kang, K S
AU  - Kang KS
AD  - c Bio Division , Medikinetics, Inc ., Pyeongtaek-si , Korea.
FAU - Park, T S
AU  - Park TS
AD  - a Graduate School of International Agricultural Technology , Seoul National 
      University , Pyeongchang-gun , Korea.
AD  - b Institute of Green-Bio Science and Technology , Seoul National University , 
      Pyeongchang-gun , Korea.
LA  - eng
PT  - Journal Article
DEP - 20181203
PL  - England
TA  - Br Poult Sci
JT  - British poultry science
JID - 15740290R
RN  - 0 (Avian Proteins)
RN  - 0 (Forkhead Box Protein O3)
SB  - IM
MH  - Animals
MH  - Avian Proteins/*genetics/metabolism
MH  - Cell Differentiation
MH  - Chick Embryo
MH  - Chickens/genetics/*physiology
MH  - Forkhead Box Protein O3/*genetics/metabolism
MH  - Gene Knockdown Techniques
MH  - Male
MH  - Muscle Development/*genetics
MH  - Muscle Fibers, Skeletal/*physiology
MH  - Myoblasts/*physiology
OTO - NOTNLM
OT  - Chicken
OT  - FOXO3
OT  - knock-down
OT  - miRNA
OT  - muscle differentiation
EDAT- 2018/11/15 06:00
MHDA- 2019/05/06 06:00
CRDT- 2018/11/15 06:00
PHST- 2018/11/15 06:00 [pubmed]
PHST- 2019/05/06 06:00 [medline]
PHST- 2018/11/15 06:00 [entrez]
AID - 10.1080/00071668.2018.1547362 [doi]
PST - ppublish
SO  - Br Poult Sci. 2019 Feb;60(1):23-30. doi: 10.1080/00071668.2018.1547362. Epub 2018 
      Dec 3.